M avium jcm15430

Lariatin A, nosiheptide, ohmyungsamycins, steffimycin and quinomycin A were purified from a culture broth of actinomycetes in our laboratory. Clarithromycin (CAM), amikacin (AMK), imipenem (IPM) and ciprofloxacin (CPFX) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Middlebrook 7H9 broth (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) containing 0.05% Tween 80 (Tokyo Chemical Industries: Tokyo, Japan) and 10% albumin dextrose catalase (ADC) enrichment (5% bovine serum albumin, Sigma Aldrich (MO, USA); 2% glucose, Wako Pure Chemical Industries (Osaka, Japan); 0.85% NaCl, Wako Pure Chemical Industries) was used for the cultivation of mycobacteria. Third-molting larvae stage silkworms, Bombyx mori (Hu•yo  ×  Tukuba•Ne), were purchased from Ehime Sanshu (Ehime, Japan). Silk Mate 2S, an artificial diet containing antibiotics, was purchased from Nosan Corporation (Kanagawa, Japan). The following mycobacterial strains were used in this study: M. avium JCM15430, M. intracellulare JCM6384, M. bovis BCG Pasteur and My. abscessus ATCC19977. The M. bovis BCG Pasteur used was a laboratory strain. M. avium JCM15430 and M. intracellulare JCM6384 were purchased from the Riken BioResource Research Center (Ibaraki, Japan). My. abscessus ATCC19977 was purchased from the American Type Culture Collection (VA, USA).