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Ab172731

Manufactured by Abcam
Sourced in United Kingdom

Ab172731 is a primary antibody product from Abcam. It is a rabbit monoclonal antibody designed to detect the target protein.

Automatically generated - may contain errors

2 protocols using ab172731

1

Immunofluorescence and Immunohistochemistry Protocol

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IF and IHC was performed overnight keeping the slides in humified chambers11 (link) and incubating them with the following primary antibodies: ΔNp63 (ab172731, Abcam, 1:500), GFP (ab13970, Abcam, 1:200), Ki67 (ab15580, Abcam, 1:1,000), cytokeratin 5 (ab53121, Abcam, 1:250), acetylated tubulin (T7451, Sigma-Aldrich, 1:200), mucin 5ac (MA1-21907, Thermo Fisher Scientific, 1:100), cleaved caspase 3 (9661, Cell Signaling, 1:200), CC10 (sc-25555, Santa Cruz, 1:200), SPC (sc-7705, Santa Cruz, 1:100) and NGFR (ab8874, Abcam, 1:100).
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2

Immunohistochemical Analysis of p63 and p40

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Two antibodies were used in this study, mouse monoclonal p63 antibody (Clone DAK-p63, Ready-To-Use, Code IR662, Dako, Glostrup, Denmark) and mouse monoclonal p40 antibody (1:100, clone BC28, Code Ab172731, Abcam, Cambridge, UK). Normal skin and laryngeal squamous cell carcinoma tissues were used as the positive controls for p63 and p40, respectively.
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