The ATP-vesicles were provided by Avanti Polar Lipids Inc. (Alabaster, AL) and provided to us in a freeze-dried form [5 (link)]. The ATP-vesicles were reconstituted with normal saline or 1640 RPMI medium (MP Biomedicals, Solon, OH) before use. After reconstitution, the composition was: 100 mg/ml of soy phosphatidylcholine (Soy PC)/1, 2-dioleyl-3-trimethylammonium-propane (DOTAP) (50:1), trehalose/Soy PC (2:1), 10 mM KH2PO4, and 10 mM Mg-ATP. The diameters of the lipid vesicles were evaluated with a Shimadzu SALD-7500 Nano Particle Size Analyzer (Shimadzu Scientific Instruments, Columbia, MD) and ranged from 85 to 150 nm.
Recombinant human platelet-derived growth factor-BB (rhPDGF-BB, becaplermin topical gel, Regranex) was made by Healthpoint Biotherapeutics (Fort Worth, TX). Mg-ATP was purchased from Sigma-Aldrich (St. Louis, MO), human CD14-FITC and human HLA-DR-PE antibodies were purchased from BD Bioscience (San Jose, CA), Anti-MAC387 was from AbD Serotec (Raleigh, NC), Anti-Collagen 1 antibodies were from Abcam (Cambridge, MA).
Recombinant human platelet-derived growth factor-BB (rhPDGF-BB, becaplermin topical gel, Regranex) was made by Healthpoint Biotherapeutics (Fort Worth, TX). Mg-ATP was purchased from Sigma-Aldrich (St. Louis, MO), human CD14-FITC and human HLA-DR-PE antibodies were purchased from BD Bioscience (San Jose, CA), Anti-MAC387 was from AbD Serotec (Raleigh, NC), Anti-Collagen 1 antibodies were from Abcam (Cambridge, MA).