SLAMF1 mutant and wild-type Farage cells were seeded at 2×105 cells/mL and cultured for 5 days. Cells were washed with 1× PBS and stained with fluorescein isothiocyanate (FITC)-conjugated anti- Annexin V antibody (BD Biosciences, Heidelberg, Germany) and propidium iodide (PI) (Sigma Aldrich, St. Louis, MO, USA). Detection of early apoptotic (Annexin V-FITC+ and PI-) and late apoptotic cells (Annexin V-FITC+ and PI+) was performed using a FACS Calibur (Becton and Dickinson). Data were analyzed with CellQuest software (Becton Dickinson).
Fitc conjugated anti annexin 5 antibody
Manufactured by BD
Sourced in Germany, United States
The FITC-conjugated anti-annexin V antibody is a laboratory reagent used in flow cytometry and other immunoassays. It binds to annexin V, a protein that interacts with phosphatidylserine and is commonly used as a marker for apoptosis. The FITC (fluorescein isothiocyanate) label allows for fluorescent detection of the antibody-annexin V complex.
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Lab products found in correlation
Facscalibur, by BD (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Cellquest software, by BD (1 mentions)
Cytomics fc500, by Beckman Coulter (1 mentions)
Igg1 fitc, by BD (1 mentions)
Cd45 percp, by BD (1 mentions)
Flow count fluorosphere, by Beckman Coulter (1 mentions)
Igg1 percp, by BD (1 mentions)
Cd41a fitc, by BD (1 mentions)
Facsverse flow cytometer, by BD (1 mentions)
3 protocols using fitc conjugated anti annexin 5 antibody
1
Apoptosis analysis of SLAMF1 mutant cells
2
Multiplex Flow Cytometry for Plasma Microparticle Analysis
3
Apoptosis Analysis by Flow Cytometry
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To analyze apoptosis, flow cytometry was performed after annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) staining, as previously reported [31] [32] [33] .
Transfected cells (5 × 10 4 ) were resuspended in 20 μL of annexin V-binding buffer (10 mM HEPES, pH 7.4; 140 mM NaCl; and 2.5 mM CaCl2) and mixed with 10 μL of FITC-conjugated anti-annexin V antibody (BD Biosciences, Franklin Lakes, NJ, USA).
The mixture was then incubated in the dark at room temperature for 20 min and PI (5 μL) was added to the cell suspension immediately prior to analysis. Finally, the relative number of annexin V-and PI-positive cells was determined using a BD FACSVerse™ flow cytometer (BD Biosciences).
Transfected cells (5 × 10 4 ) were resuspended in 20 μL of annexin V-binding buffer (10 mM HEPES, pH 7.4; 140 mM NaCl; and 2.5 mM CaCl2) and mixed with 10 μL of FITC-conjugated anti-annexin V antibody (BD Biosciences, Franklin Lakes, NJ, USA).
The mixture was then incubated in the dark at room temperature for 20 min and PI (5 μL) was added to the cell suspension immediately prior to analysis. Finally, the relative number of annexin V-and PI-positive cells was determined using a BD FACSVerse™ flow cytometer (BD Biosciences).
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