Amplified viral DNA, cDNA and total microbial DNA were used for shotgun library construction. DNA concentration was measured by Quant-iT PicoGreen dsDNA Assay kit (Thermo Fisher Scientific), and the fluorescence was detected by EnVision Multilabel Plate Reader (Waltham, MA, USA). Libraries were made using an Illumina Nextera XT Samples Prep kit (Illumina, San Diego, CA, USA), quantified using both Quant-iT PicoGreen dsDNA Assay kit and KAPA Library Quantification kit (Kapa Biosystems, Basel, Switzerland). The size distribution of the libraries was checked by 5300 Fragment Analyzer (Agilent, Santa Clara, CA, USA). Libraries were pooled for sequencing. The concentration of the pooled libraries was measured using Qubit (Invitrogen, Carlsbad, CA, USA), and the size distribution of the pooled libraries was checked by Agilent Technology 2100 Bioanalyzer using a High Sensitivity DNA chip (Agilent). Sequence was acquired using the Illumina Miseq (250 bp paired-end reads, Illumina) and HiSeq (150 bp paired-end reads, Illumina).
Envision multilabel plate reader
Manufactured by Thermo Fisher Scientific
Sourced in United States
The EnVision Multilabel Plate Reader is a versatile and high-performance instrument designed for various plate-based assays. It offers multiple detection modes, including absorbance, fluorescence, luminescence, and time-resolved fluorescence, enabling a wide range of applications within the laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Nextera xt samples prep kit, by Illumina (2 mentions)
Quant it picogreen dsdna assay kit, by Roche (2 mentions)
Fragment analyzer 5300, by Agilent Technologies (2 mentions)
Agilent technology 2100 bioanalyzer, by Agilent Technologies (2 mentions)
Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (2 mentions)
Kapa library quantification kit, by Roche (2 mentions)
High sensitivity dna chip, by Agilent Technologies (2 mentions)
2 protocols using envision multilabel plate reader
1
Shotgun Sequencing of Diverse DNA Samples
2
Shotgun Sequencing of Diverse DNA Samples
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Amplified viral DNA, cDNA and total microbial DNA were used for shotgun library construction. DNA concentration was measured by Quant-iT PicoGreen dsDNA Assay kit (Thermo Fisher Scientific), and the fluorescence was detected by EnVision Multilabel Plate Reader (Waltham, MA, USA). Libraries were made using an Illumina Nextera XT Samples Prep kit (Illumina, San Diego, CA, USA), quantified using both Quant-iT PicoGreen dsDNA Assay kit and KAPA Library Quantification kit (Kapa Biosystems, Basel, Switzerland). The size distribution of the libraries was checked by 5300 Fragment Analyzer (Agilent, Santa Clara, CA, USA). Libraries were pooled for sequencing. The concentration of the pooled libraries was measured using Qubit (Invitrogen, Carlsbad, CA, USA), and the size distribution of the pooled libraries was checked by Agilent Technology 2100 Bioanalyzer using a High Sensitivity DNA chip (Agilent). Sequence was acquired using the Illumina Miseq (250 bp paired-end reads, Illumina) and HiSeq (150 bp paired-end reads, Illumina).
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