For surface staining, T cells were washed in FACS buffer containing PBS and 5% foetal bovine serum followed by incubation for 20 minutes at 4°C with fluorophore-conjugated antibodies against the several surface markers selected. The following antibodies were used: anti-CD4 APC-Cy7, anti-CD8 Pacific Blue, anti-TMEM123 APC and PE, anti-LFA1 APC, anti-ICAM1 APC, anti-CD44 FITC, anti-CD62L PE-Cy-5, anti-CCR7 PE-Cy-7, anti-PD1 BV711, anti-CD39 BUV563, anti-TIM-3 BV650, anti-CD69 PE-Cy-7 (all from BD Biosciences). Cytokine production was assessed by intracellular staining. The cells were fixed and permeabilized with Cytofix/Cytoperm (BD Bioscience) and then stained with anti-IFN-γ PE/Cy-5, anti-TNFα APC, anti-IL-2 FITC and anti-IL22 PE-Cy-7 (from BD Biosciences). Samples were acquired using a FACS Canto-II flow cytometer (BD Biosciences) and data were analysed using FlowJo software version 10 (LLC).
Anti pd1 bv711
Manufactured by BD
Anti-PD1 BV711 is a fluorochrome-conjugated antibody used in flow cytometry applications for the detection and analysis of PD-1 (Programmed Cell Death Protein 1) expressing cells. It provides a specific and sensitive tool for immunophenotyping and characterization of PD-1 positive cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Anti il 2 fitc, by BD (1 mentions)
Anti cd44 fitc, by BD (1 mentions)
Anti ccr7 pe cy7, by BD (1 mentions)
Anti tnf α apc, by BD (1 mentions)
Anti tim 3 bv650, by BD (1 mentions)
Anti icam 1 apc, by BD (1 mentions)
Anti cd8 pacific blue, by BD (1 mentions)
Anti cd69 pe cy7, by BD (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Cytofix cytoperm, by BD (1 mentions)
Anti cd4 apc cy7, by BD (1 mentions)
Anti cd62l pe cy 5, by BD (1 mentions)
Anti ctla4 pe cy7, by BioLegend (1 mentions)
Lsrfortessa flow cytometer, by BD (1 mentions)
2 protocols using anti pd1 bv711
1
Comprehensive Immune Cell Profiling by Flow Cytometry
2
Immunophenotyping of T cell subsets
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Evaluation of T cell surface markers was performed via immunofluorescence staining and analyzed via flow cytometry, as previously described.24 Additional monoclonal antibodies used to characterize T cells include: anti–TIM-3-BV650, anti–CTLA-4-PE Cy7, and anti–LAG-3-FITC (BioLegend, San Diego, CA) as well as anti–PD-1-BV711, anti–CD69-AF700, and anti–CD39-PE (BD Biosciences). Flow cytometry was performed using the LSR Fortessa flow cytometer (BD Biosciences). Data were analyzed with FlowJo software.
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