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Pyromark pcr mix

Manufactured by Qiagen
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PyroMark PCR Mix is a ready-to-use solution for performing polymerase chain reaction (PCR) amplification. It contains all the necessary components, including a thermostable DNA polymerase, dNTPs, and optimized buffer, for efficient DNA amplification.

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Lab products found in correlation

Pyromark q48 autoprep, by Qiagen (2 mentions) Dneasy blood tissue kit, by Qiagen (2 mentions)

Spelling variants of this product

PyroMark PCR Master Mix (22 citations) PyroMark® PCR Master Mix 2x (3 citations) PyroMark PCR Master Mix kit (3 citations)

2 protocols using pyromark pcr mix

1

DNA Extraction and PyroMark PCR Amplification

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DNA was extracted from cell pellets using the DNeasy Blood & Tissue Kit (Qiagen) as per the manufacturer’s instructions. PCR was then performed using the PyroMark PCR Mix (Qiagen) for 50 cycles with an annealing temperature of 50 °C and an extension time of 30 s. PCR products were run on the PyroMark Q48 Autoprep (Qiagen) as per the manufacturer’s instructions.
Mahmood M., Liu E.M., Shergold A.L., Tolla E., Tait-Mulder J., Huerta-Uribe A., Shokry E., Young A.L., Lilla S., Kim M., Park T., Boscenco S., Manchon J.L., Rodríguez-Antona C., Walters R.C., Springett R.J., Blaza J.N., Mitchell L., Blyth K., Zanivan S., Sumpton D., Roberts E.W., Reznik E, & Gammage P.A. (2024). Mitochondrial DNA mutations drive aerobic glycolysis to enhance checkpoint blockade response in melanoma. Nature Cancer, 5(4), 659-672.
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2

Mitochondrial DNA Variant Profiling

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DNA was extracted from cell pellets using the DNeasy Blood & Tissue Kit (Qiagen) as per the manufacturer’s instructions. PCR was then performed using the PyroMark PCR Mix (Qiagen) for 50 cycles with an annealing temperature of 50°C and an extension time of 30sec. PCR products were run on the PyroMark Q48 Autoprep (Qiagen) as per the manufacturer’s instructions.
PCR primers for mt.12,436
Forward: 5’-ATATTCTCCAACAACAACG-3’
Reverse: 5’-biotin-GTTATTATTAGTCGTGAGG-3’
PCR primers for mt.11,944
Forward: 5’-CTTCATTATTAGCCTCTTAC-3’
Reverse: 5’-biotin-GTCTGAGTGTATATATCATG-3’
Sequencing primer for mt.12,436
5’-TTGGCCTCCACCCAT-3’
Sequencing primer for mt.11,944
5’-TAATTACAACCTGGCACT-3’
Mahmood M., Liu E.M., Shergold A.L., Tolla E., Tait-Mulder J., Huerta Uribe A., Shokry E., Young A.L., Lilla S., Kim M., Park T., Manchon J.L., Rodríguez-Antona C., Walters R.C., Springett R.J., Blaza J.N., Zanivan S., Sumpton D., Roberts E.W., Reznik E, & Gammage P.A. (2023). Tumour mitochondrial DNA mutations drive aerobic glycolysis to enhance checkpoint blockade. bioRxiv.
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