The House Ear Institute-Organ of Corti 1 (HEI-OC1) cells were cultured in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS, Gibco, #10,437,028) without antibiotics at 33°C and 10% CO2.
LncRNA Gm44593 overexpression plasmid was purchased from Gene Pharma (Shanghai, China). Wild type and mutant lncRNA Gm44593 vector were constructed by Gene Pharma (Shanghai, China). MiR-29b mimics and inhibitor were purchased from Gene Pharma (Shanghai, China). All transfection experiments were performed via lipo 3000 reagent (Invitrogen) according to the manufacturer’s protocol. 500um H2O2 were treated for 3 hours.
LncRNA Gm44593 overexpression plasmid was purchased from Gene Pharma (Shanghai, China). Wild type and mutant lncRNA Gm44593 vector were constructed by Gene Pharma (Shanghai, China). MiR-29b mimics and inhibitor were purchased from Gene Pharma (Shanghai, China). All transfection experiments were performed via lipo 3000 reagent (Invitrogen) according to the manufacturer’s protocol. 500um H2O2 were treated for 3 hours.