Ammelide

Manufactured by Merck Group

Ammelide is a laboratory equipment product manufactured by Merck Group. It is a chemical compound used in various scientific and research applications. The core function of Ammelide is to serve as a reagent or intermediate in chemical reactions and analyses.

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Lab products found in correlation

Cyanuric acid, by Merck Group (2 mentions) Simazine, by Merck Group (1 mentions) Ammonium hydroxide, by Merck Group (1 mentions) Ethyl acetate, by Avantor (1 mentions) Ammonium formate, by Merck Group (1 mentions) Melamine, by Merck Group (1 mentions) Hplc grade acetonitrile, by Avantor (1 mentions)

2 protocols using ammelide

Isolation and Characterization of Simazine-Degrading Bacteria

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Chemicals: simazine (98.6%), ammelide (99.0%) and cyanuric acid (99.5%) were purchased from Sigma-Aldrich Company and Dr. Ehrenstorfer GmbH. Other chemicals used were of analytical grade.
Media: (I). Mineral salt medium (Lanyi Biotechnology Co., Ltd., China): Ammonium sulfate 0.1 g, Dipotassium hydrogenphosphate 0.1g, Calcium sulfate 0.05 g, Ferrous sulfate heptahydrate 0.01 g, Magnesium sulfate heptahydrate 0.2 g, H 2 O 1.0 L, pH 7.0. (II). Isolation medium: mineral salt medium containing 5.0 mg/L simazine. (III). LB (Luria-Bertani) medium (Lanyi Biotechnology Co., Ltd.): yeast extract 5 Bras Cienc (2021) 93(Suppl. 3) e20210373 4 | 17 g, peptone 10 g, NaCl 10 g, H 2 O 1.0 L, pH7.0. (IV). Enrichment medium (Lanyi Biotechnology Co., Ltd.): beef extract 5 g, yeast extract 5 g, peptone 7 g, NaCl 5 g, H 2 O 1.0 L, pH7.0.

Zhu J., Zhao Y., Li X., Wu L., Fu L.I., Yang N., Yin J, & Huang R. (2021). Isolation of 2 simazine-degrading bacteria and development of a microbial agent for bioremediation of simazine pollution. Anais da Academia Brasileira de Ciencias, 93(suppl 3).

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Determination of Melamine and Derivatives

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Analytical standards (melamine, ammeline, ammelide, and cyanuric acid) and corresponding isotopically labeled internal standards (ISs; ¹⁵N₃, ¹³C₃-melamine, ¹³C₃-ammeline, ¹³C₃-ammelide, and ¹⁵N₃,¹³C₃-cyanuric acid) were purchased from Sigma-Aldrich (St. Louis, MO) and Cambridge Isotope Laboratories (Andover, MA), respectively. Ammonium formate (>99.995% purity) and ammonium hydroxide (28.0–30.0% NH₃ basis; NH₄OH) were purchased from Sigma-Aldrich (St. Louis, MO). HPLC-grade acetonitrile, water, ethyl acetate, and reagent-grade formic acid (FA) were purchased from J. T. Baker (Phillipsburg, NJ). HPLC-grade isopropanol was purchased from Honeywell B&J (Morristown, NJ).

Zhu H, & Kannan K. (2018). Inter-day and inter-individual variability in urinary concentrations of melamine and cyanuric acid. Environment international, 123, 375-381.

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