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Fk 506 rapamycin

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FK-506 and rapamycin are chemical compounds commonly used in laboratory research. FK-506 is a macrolide lactone compound, while rapamycin is a triene macrolide compound. Both substances have immunosuppressant properties and are often used in studies related to cellular signaling pathways, immune system regulation, and potential therapeutic applications.

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Lab products found in correlation

Motorized injector, by Stoelting (1 mentions) Stereotactic frame, by Harvard Apparatus (1 mentions) Manual micromanipulator, by World Precision Instruments (1 mentions) Microsyringe pump controller, by World Precision Instruments (1 mentions)

2 protocols using fk 506 rapamycin

1

Intracerebral Transplantation of SPIO-labeled mESCs

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All animal procedures were performed under an approved protocol from our Institutional Animal Care and Use Committee (IACUC). Three 3-week-old male BALB/c mice (Harlan Laboratories, IN, USA) were anesthetized using 2% isoflurane, were shaved, and then immobilized in a stereotactic frame (Harvard Apparatus, MA, USA). SPIO-labeled Luc-mESCs (5 × 104 cells in 2 μL of volume) were injected into the brain using a 31-gauge needle and a motorized injector (Stoelting Co., IL, USA) at a rate of 0.5 μL/min and the following coordinates: anterior–posterior (AP) = 0 mm, medial–lateral (ML) = 2.0 mm, and dorsal–ventral (DV) = 1.5 mm. The needle was carefully withdrawn 2 minutes after the end of the injection to minimize backflow. Animals were immunosuppressed by intraperitoneal daily administration of a cocktail of (FK-506) + (rapamycin) (1 mg/kg each; LC Laboratories, Woburn, Massachusetts), beginning 3 days before cell transplantation, and then daily until sacrifice.
Chehade M., Srivastava A.K, & Bulte J.W. (2016). Co-Registration of Bioluminescence Tomography, Computed Tomography, and Magnetic Resonance Imaging for Multimodal In Vivo Stem Cell Tracking. Tomography, 2(2), 158-165.
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2

Intraspinal Transplantation of Human Cells

Cited 1 time
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Human GRPs were suspended (in basal medium) at a concentration of 7.5 × 104 cells/uL. After the completion of the transplantation session, cell viability was assessed using the trypan blue assay and was always found to be greater than 75%. Immune suppressed animals received transplants at 50-60 days of age. Animals were immune suppressed by intraperitoneal administration of FK-506/Rapamycin (1mg/kg/each; LC Laboratories; Woburn, MA) daily beginning five days before grafting and continuously until sacrifice. Each mouse received 2 grafts (bilaterally at C5) of 1.5×105 cells/site (in 2μL basal media) into the ventral horn. Cells were delivered using a 10 μL Hamilton Gastight syringe with an attached 30-gauge 45° beveled needle (Hamilton; Reno, NV). The injection pipette was secured to a manual micromanipulator (World Precision Instruments; Sarasota, FL) attached to an 80° tilting base (Lepore et al., 2008b (link)). The tip was lowered to a depth of 0.75 mm below the surface of the cord and was held in place for 2 minutes before and after cell injection. Cells were delivered under the control of a microsyringe pump controller (World Precision Instruments) at a rate of 1μL/minute.
Haidet-Phillips A.M., Doreswamy A., Gross S.K., Tang X., Campanelli J.T, & Maragakis N.J. (2014). Human glial progenitor engraftment and gene expression is independent of the ALS environment. Experimental neurology, 264, 188-199.
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