Beas 2b cell line

Human small cell lung cancer H446 cell and bronchial epithelial BEAS-2B cell lines were obtained from the American Type Culture Collection. The Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Hyclone. The CCK8 kit was from Med ChemExpress. Hoechst 33,258 dyeing liquid, cell cycle, and apoptosis detection kit were obtained from Beyotime. Hematoporphyrin derivative was purchased from Huading Modern Biologic& Chemical Co. Ltd. The reverse transcription kit and SYBR Green fluorescent quantitative PCR kit were from TaKaRa. LED-IB photodynamic therapy instrument was made by Wuhan Yage Photoelectric Technology Co. LTD (China). Mode of operation: continuous. The output wavelength was: Red light 630 nm; Output power: 80 mW/cm².

BEAS-2B cell lines were acquired from the American Type Culture Collection (ATCC, Rockville, MA, USA). C6-ceramide, ammonium pyrrolidinedithiocarbamate (PDTC), tyrphostin B42 (AG490), and 6-Nitrobenzo[b]thiophene-1, 1-dioxide (Stattic) were acquired from Sigma-Aldrich (St Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) and Fetal bovine serum (FBS) were obtained from HyClone (Logan, UT, USA). Anti-JAK2, anti-phosphorylated JAK2 (p-JAK2), anti-STAT3, anti-phosphorylated STAT3 (p-STAT3), anti-CD68, and anti-β-actin antibodies were acquired from Cell Signaling Technology (Beverly, MA, USA). IRDye 680RD Goat anti-Rabbit and Goat anti-Mouse IgG (H + L) was acquired from LI-COR Biosciences (Lincoln, NE, USA). TRIzol was acquired from Invitrogen Corporation (Carlsbad, California, USA). TB Green Mixture was acquired from TaKaRa (Otsu, Shiga, Japan).

Two human NSCLC A549 and H1299 cell lines, human embryonic fibroblast MRC-5 cell line and the human bronchial epithelial BEAS.2B cell line were purchased from the ATCC (Manassas, VA, United States). Cells were cultured in RPMI-1640 (Gibco, Grand Island, NY, United States) supplemented with 10% FBS (GIBCO, United States) and 1% penicillin/streptomycin in a humidified 5% CO₂ atmosphere at 37°C.

The Beas2B cell line was bought from ATCC (CRL-9609) and was grown in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin at 37 °C. The plasmid constructs used in this study included EGFR-mCherry, EGFR-EGFP (the cDNA encoding human EGFR were ordered from BGI (Beijing, China). The plasmids Str-KDEL_SBP-mCherry-EGFR and Str-KDEL_SBP-EGFP-EGFR were generated by standard molecular cloning procedures. The N-terminus of SBP-EGFP tag, SBP-mCherry tag are followed by a signal sequence derived from IL-2^{67 (link)}), Tomm20-EGFP (artificially constructed based on EGFP-N1 backbone), 3XmEmerald-ensconsin (a gift from Prof. Dong Li (University of Chinese Academy of Sciences), Tomm20-mCherry (artificially constructed based on mCherry-N1 backbone), EGFP-Sec61β and Halo-clathrin (gifts from Prof. Yuhui Zhang (Huazhong University of Science and Technology)). The day before transfection, cells were seeded into the wells of a 24-well plate with 500 μL culture medium. The indicated plasmid was transfected into cells by the Lipofectamine LTX (Invitrogen) according to the standard protocol. The cells were digested with 0.25% trypsin (Thermo Fisher Scientific) 6–8 h after transfection, seeded onto confocal dishes, and cultured at 37 °C with 5% CO₂ for another 24 h.

Lung cancer cell lines including A549, H1299, and H1650 cells was purchased from ATCC. The normal human bronchial epithelial (BEAS-2B) cell line was also purchased from ATCC. All the cells were cultured in the 1640 medium (Gibco, USA) with 10% FBS (HyClone Sera, USA) and 1% penicillin‐streptomycin (Sangon Biotech, China) in an atmosphere of 5% CO2 at 37 °C.The BIRC5 shRNA expression vector and scrambled shRNA nontarget control were obtained from Genewiz. Plasmids (Genewiz, China) were transfected through Lipofectamine 3000 (Thermo Scientific, USA) following the instructions from the manufacturer’s protocols.

Human non-small-cell lung
cancer cell lines A549
and H1975 and the human bronchial epithelial BEAS-2B cell line were
purchased from ATCC (Manassas, VA). Cells were cultured in Roswell
Park Memorial Institute (RPMI)-1640 media supplemented with 10% v/v
fetal bovine serum, 100 U·mL^–1 penicillin,
and 100 μg·mL^–1 streptomycin. Cells were
grown in a humidified atmosphere at 37 °C and 5% CO₂.