Anti rock2

One losartan potassium tablet (50 mg, 461.01 g/mol, Merck Sharp & Dohme (Australia) Pty., Ltd.) was dissolved in 9375 μl of saline for intragastric administration, and one tablet was dissolved in 1084.6 μl of PBS for cell experiments.
The following primary antibodies were used: anti-CXCR4 (WB: ABclonal, A1303; IHC: A12534); anti-FAK (CST, 3285); anti p-FAK (CST, 3282); anti-AGTR1 (LifeSpan, LS-B4614-50); anti-RhoA (Santa Cruz, sc-418); anti-ROCK1 (Santa Cruz, sc-374388); anti-ROCK2 (ABclonal, A5698); MLC (Abcam, 3672); and p-MLC (Abcam, 3675).

5-Bromo-20-deoxyuridine (BrdU), glial fibrillary acidic protein (GFAP), ionized calcium binding adapter molecule 1 (Iba1), and neurofilament-200(NF200) antibodies were purchased from Abcam (MC, UK). Ras homolog gene family, member A (RhoA), anti-C3d, anti-NGR, interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor α (TNFα), growth associated protein-43 (GAP43), and anti-GAPDH antibodies were purchased from Affinity (OH, USA). S100 calcium-binding protein A10 (S100A10) and anti-ROCK2 were purchased from ABclonal Technology Co., Ltd. (Wuhan, China). CSPGs was purchased from Merck Chemicals (Shanghai, China) Co., Ltd. Neuron-specific nuclear protein (NeuN) was purchased from Novus (Co, USA). Antitubulin was purchased from Proteintech (IL, USA). Y-27632 dihydrochloride was purchased from APExBIO (Tx, USA).