EG (2.7 kg) was initially soaked in 70% ethanol (Xilong Science) and then decocted to an extract solution, followed by rotary evaporation of the extract solution under reduced pressure. Subsequently, the concentrated extracts were further extracted with petroleum ether (Xilong Science), and then the petroleum ether extract and the residue fraction were obtained, respectively. The residue fraction was concentrated under reduced pressure and then separated by macroporous resin column chromatography (Baoen Chemical) and eluted with a gradient system of water-ethanol (100:0 and 70:30). The resultant 30% ethanol fraction of EG extract (EEEG) was harvested and evaporated with a rotary evaporator under reduced pressure followed by vacuum freeze drying. Before in vitro studies, EEEG was dissolved in DMSO (Solarbio) to get a stock solution followed by further dilution to a final concentration with the cell culture medium. For animal experiments, EEEG was dissolved in 0.1% CMC-Na (Zhanyun Chemical).
Petroleum ether
Manufactured by Xilong
Sourced in China
Petroleum ether is a volatile, colorless liquid used as a solvent and extracting agent in various laboratory applications. It is a mixture of aliphatic hydrocarbons with a boiling point range typically between 30°C and 60°C. Petroleum ether is commonly used for the extraction and purification of organic compounds due to its selective solvent properties.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Xilong (4 mentions)
Ethyl acetate, by Xilong (4 mentions)
Sodium hydroxide (naoh), by Xilong (3 mentions)
Hydrochloric acid (hcl), by Sinopharm (3 mentions)
Methanol, by Xilong (3 mentions)
N butanol, by Xilong (3 mentions)
Acetone, by Xilong (2 mentions)
Hexane, by Xilong (2 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Dichloromethane, by Xilong (1 mentions)
Anhydrous sodium sulfate, by Xilong (1 mentions)
Silver nitrate, by Xilong (1 mentions)
Glycerol, by Xilong (1 mentions)
Methyl orange, by Sinopharm (1 mentions)
Sodium bisulfite, by Sinopharm (1 mentions)
Potassium sodium tartrate, by Sinopharm (1 mentions)
Lb medium, by Sinopharm (1 mentions)
Phenolphthalein, by Sinopharm (1 mentions)
Glycerin, by Xilong (1 mentions)
Absolute ethanol, by Xilong (1 mentions)
15 protocols using petroleum ether
1
Extraction and Purification of EG Compound
2
Extraction and Quantification of Prochloraz and 2,4,6-TCP
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Prochloraz (98.0%) and 2,4,6-TCP (98.5%) were purchased by Dr. Ehrenstorfer GmbH. The commercial formulations of 25% prochloraz emulsifiable concentrate (EC) were provided by Guoguang Agrochemical Ltd. (Jianyang, Sichuan, China). Acetone, hexane, petroleum ether, dichloromethane, and anhydrous sodium sulfate of analytical grade were purchased from Xilong Chemical Co., Ltd. (Shanghai, China).
An EYELA N-1100 rotary evaporator was used from the Shanghai Ailang Instruments Co., Ltd. (Shanghai, China). An SC-3610 low-speed centrifuge was used from Anhui Zhongke Zhongjia Scientific Instruments Inc. (Hefei, Anhui, China). A KQ-5200 ultrasonic cleaner was used from the Kunshan Ultrasonic Instrument Co., Ltd. (Shanghai, China). An SQ-2119B multifunctional food processing instrument was used from the Shanghai Shuaijia Electronic Technology Co., Ltd. (Shanghai, China). A DHG-9070A electrothermal oven thermostat blast was used from the Shanghai Yiheng Science and Technology Co., Ltd. (Shanghai, China). An SY-2 digital display room temperature sand bath was used from the Jintan Chenghui Instrument Factory (Jiangsu, China).
An EYELA N-1100 rotary evaporator was used from the Shanghai Ailang Instruments Co., Ltd. (Shanghai, China). An SC-3610 low-speed centrifuge was used from Anhui Zhongke Zhongjia Scientific Instruments Inc. (Hefei, Anhui, China). A KQ-5200 ultrasonic cleaner was used from the Kunshan Ultrasonic Instrument Co., Ltd. (Shanghai, China). An SQ-2119B multifunctional food processing instrument was used from the Shanghai Shuaijia Electronic Technology Co., Ltd. (Shanghai, China). A DHG-9070A electrothermal oven thermostat blast was used from the Shanghai Yiheng Science and Technology Co., Ltd. (Shanghai, China). An SY-2 digital display room temperature sand bath was used from the Jintan Chenghui Instrument Factory (Jiangsu, China).
3
Antimicrobial Cassava Starch Biopolymer
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Cassava starch and octenyl succinate cassava starch ester are self-made in the laboratory; Chitosan (degree of deacetylation ≥ 90%) comes from Hefei Bomei Biotechnology Co., Ltd; Octenyl succinic anhydride comes from Guangzhou Guangjia Chemical Co., Ltd; Zinc oxide (particle size 30 nm) comes from cool chemistry; Sodium hydroxide, silver nitrate, glycerol, petroleum ether and absolute ethanol all come from Xilong science; 3,5-Dinitrosalicylic acid comes from Tianjin Dalong Chemical Reagent Factory; ε- Polylysine, hydrochloric acid, phenolphthalein, crystalline phenol, sodium bisulfite, potassium sodium tartrate, LB medium, agar and methyl orange were all purchased from Sinopharm Chemical Reagent Co., Ltd. PBS buffer solution (pH = 6.8) was purchased from Lvyuan Biotechnology Co., Ltd. the reagents were all analytical pure. Mouse embryonic fibroblasts) are from Wuhan prosai biotechnology company; Fetal bovine serum was purchased from Hangzhou Sijiqing; DMEM culture medium was purchased from Beijing solebao; Cell counting kit-8 was purchased from Shanghai Yisheng; E.coli and S.aureus come from Guangdong huankai Biotechnology Co., Ltd.
4
Biopolymer-Based Fruit Preservation
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Cassava starch and octenyl succinate cassava starch ester are self-made in the laboratory; Chitosan (degree of deacetylation ≥90 %) was purchased from Hefei Bomei Biotechnology Co., ltd; Octenylsuccinic anhydride comes from Guangzhou Guangjia Chemical Co., ltd; Zinc oxide (particle size 30 nm) comes from cool chemistry; Sodium hydroxide, glycerin, petroleum ether and absolute ethanol are all purchased from Xilong science; ε- Polylysine and hydrochloric acid came from Sinopharm Chemical Reagent Co., ltd. and PBS buffer was purchased from Lvyuan Biotechnology Co., ltd. All reagents are analytically pure. Sweet cherries were purchased from the orchard of Hainan University; The manufacturer of PE fresh-keeping film is yueshitang.
5
Synthesis of Polymer-based Adsorbent
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Styrene (St), divinylbenzene (DVB), azobisisobutyronitrile (AIBN), span 80, and ethyl acetate (EAC) were purchased from Shanghai Macklin Biochemical Co., Ltd. Carbon tetrachloride (CTC), dichloromethane (DCM) and trichloromethane were obtained from Chengdu Kelong Chemical Co., Ltd. Hydrochloric acid and toluene were procured from Sinopharm Chemical Reagent Co., Ltd. Petroleum ether and methanol were sourced from Xilong Chemical Co., Ltd. Diesel and corn oil were bought locally in Zhanjiang, and oyster shell powders (OSPs18 (link)) were obtained by self-production. Distilled water was provided by the chemistry laboratory center of the esteemed institution.
6
Extraction and Characterization of MT Biomass
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The MT was harvest at Guangdong, China. Ethanol, acetone, petroleum ether and paraffin were obtained from Xilong Chemical Co., Ltd. (Guangzhou, China). Methylene blue and Sudan III were provided by Kermel Chemical Reagent Co., Ltd. Tianjin, China. 3-ethyl-1-methyl- imidazolium acetate ([Emim]OAc, 98%) was purchased from Qingdao Ionike New Material Technology Co., Ltd. (Qingdao, China). Methyl silicone oil and engine oil were supplied by Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Soybean oil was purchased from a local market.
7
Synthesis of Azobenzene-Based Monomers
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Methacrylamido-azobenzene (MAAAB) and 2-[(2′-tetrahydropyranyl)oxy]ethylacrylate (THP-HEA) monomer were prepared in our laboratory according to previous reports [26 ,27 (link)]. Absolute diethyl ether (AR, XilongScientific, Shantou, China), ethyl acetate (AR, XilongScientific, Shantou, China), petroleum ether (AR, XilongScientific, Shantou, China), concentrated hydrochloric acid (HCl, AR, Aladdin, Shanghai, China), methanol (AR, XilongScientific, Shantou, China), Nile red (NR, 95.0%, Aladdin, Shanghai, China), N,N-dimethyl formamide (DMF, 95%, XilongScientific, Shantou, China) were used without further purification. Tetrahydrofuran (THF, 99.8%, Sinopharm Chemical Reagent, Shanghai, China) was refluxed with sodium and distilled. 2,2′-Azobis (isobutyronitrile) (AIBN, 98.0%, Aladdin) was recrystallized from absolute ethanol and stored in refrigerator.
8
Phytochemical Extraction and Cytokine Evaluation
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Zheshen No. 2 P. Heterophylla (Miq.) Pax Chinese herbal was purchased from Ningde Nanling Agricultural Co., Ltd. Zherong County of the Ningde region of the Fujian Province in China is the advocate producing area of TZS. The TZS was cut into pieces and then dried below 60°C.
Chromatographic pure methanol was purchased from Merck KGaA (Darmstadt, Germany). Chromatographic pure acetonitrile was purchased from Fisher Scientific UK Ltd. (Leicestershire, England). Analytical grade reagents including petroleum ether, ethyl acetate, ethanol, n-butanol, ammonia, and chloroform were bought from Xilong Scientific Co., Ltd. (Shantou, China). Interleukin-10 (IL-10) and tumor necrosis factor (TNF-α) enzyme linked immunosorbent assay (ELISA) kits were purchased from cloud-clone corp.
Chromatographic pure methanol was purchased from Merck KGaA (Darmstadt, Germany). Chromatographic pure acetonitrile was purchased from Fisher Scientific UK Ltd. (Leicestershire, England). Analytical grade reagents including petroleum ether, ethyl acetate, ethanol, n-butanol, ammonia, and chloroform were bought from Xilong Scientific Co., Ltd. (Shantou, China). Interleukin-10 (IL-10) and tumor necrosis factor (TNF-α) enzyme linked immunosorbent assay (ELISA) kits were purchased from cloud-clone corp.
9
Bamboo Pulp Modification and Characterization
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Bamboo bleached pulp (molecular weight (Mη), 1.9 × 105–2.2 × 105, alpha–fiber content: 86–88%) was supplied by Ganzhou Hwagain Co., Ltd. (Ganzhou, China) (Table S1 ). Sodium hydroxide (96%, AR), urea (99%, AR) and hexadecyltrimethoxysilane (HDTMS, 99%, AR) were obtained from Shanghai Aladdin Bio–Chem Technology Corporation (Shanghai, China). The 1,2–dichloroethane (99%, AR), t–butanol (TBA, 99%, AR), para–xylene (99%, AR), toluene (99.5%, AR), benzene (99.5%, AR), cyclohexane (99.5%, AR), petroleum ether (AR) and hexane (97%, AR) were purchased from Xilong Chemical Co., Ltd. (Beijing, China). Vacuum pump oil was purchased from the Beijing Sifang Special Oil Factory (API: CS 100, Beijing, China). Peanut oil was obtained from the local supermarket (Unsaturated fatty acid content 80%, Yimengshan Peanut Oil Co., Ltd. Linyi, China). Oil Red O was purchased from Bio–lab Technology Co., Ltd. (Dye content ≥ 75%, Beijing, China). Deionized water was made in the laboratory and used for all experiments and tests. All reagents were used as–received without further purification.
10
Turmeric Compound Extraction and Purification
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Turmeric was procured from Jiangxi Zhangshu Tianqitang Traditional Chinese Medicine Pieces (Zhangshu, China), HP-β-CD from Shandong Binzhou Huike Cyclodextrin Technology (Binzhou, China), petroleum ether from Xilong Science (Shantou, China), and anhydrous sodium sulfate and methanol from Sinopharm Chemical Reagent (Shanghai, China). Moreover, sodium chloride, anhydrous calcium chloride, and anhydrous potassium carbonate were all purchased from Shanghai Macleans Biochemical Technology (Shanghai, China).
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