4t1 breast cancer cell line

Manufactured by American Type Culture Collection

Sourced in United States

The 4T1 breast cancer cell line is a murine mammary carcinoma cell line derived from a spontaneously arising tumor in a BALB/c mouse. It is a commonly used model for studying breast cancer metastasis.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

4T1 cells (122 citations) 4T1 cell line (46 citations) 4T1 breast cancer cells (12 citations) 4T1 breast cancer (4 citations)

6 protocols using 4t1 breast cancer cell line

4T1 Breast Cancer Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 4T1 breast cancer cell line was obtained from the American Type Culture Collection (ATCC), Manassas, VA. Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS) (R&D Systems, S11150H, Minneapolis, MN, USA) and 1% (v/v) combination of 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, 15140-122, Carlsbad, CA, USA). Cells were cultured at 37 °C under humidified 5% CO2 conditions.

Caillaud M., Patel N.H., Toma W., White A., Thompson D., Mann J., Tran T.H., Roberts J.L., Poklis J.L., Bigbee J.W., Fang X., Gewirtz D.A, & Damaj M.I. (2020). A Fenofibrate Diet Prevents Paclitaxel-Induced Peripheral Neuropathy in Mice. Cancers, 13(1), 69.

+ Open protocol

+ Expand

Establishment of Murine 4T1 Breast Cancer Cell Line Expressing ROR1

Check if the same lab product or an alternative is used in the 5 most similar protocols

The murine 4T1 breast cancer cell line (American Type Culture Collection, Cat# CRL-2539) was cultured in complete RPMI 1640 medium containing 10% heat-inactivated fetal bovine serum (FBS), 2 mM L-glutamine, 1.5 g/l sodium bicarbonate, 4.5 g/l glucose, 1.0 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol, and 10 mM HEPES buffer (pH 7.2–7.5). To generate a 4T1 tumor cell line that expresses murine ROR1 (4T1-ROR1), we transfected cells with the ROR1 lentiviral vector (pLenti-GIII-CMV-GFP-2A-Puro) marketed by abmgood.com (Cat# LV536399). Tumor cells were cultured in complete medium containing 10 µg/ml puromycin (Invivogen) for three weeks. The Platinum-E (Plat-E) retroviral packaging cell line (Cell Biolabs, Cat# RV-101) was cultured in DMEM containing 10% FBS, 2 mM glutamate, 100 U/ml penicillin, and 100 µg/ml streptomycin. To ensure high retroviral titer, only Plat-E cells passaged no more than 5 times were used for gene transfer. Cell line authentication was not performed. All cell lines tested negative for mycoplasma using a DNA-based PCR test (DDC Medical).

Zhang F., Stephan S.B., Ene C.I., Smith T.T., Holland E.C, & Stephan M.T. (2018). Nanoparticles that reshape the tumor milieu create a therapeutic window for effective T cell therapy in solid malignancies. Cancer research, 78(13), 3718-3730.

+ Open protocol

+ Expand

4T1 Breast Cancer Mouse Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 4T1 breast cancer cell line purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) was grown in Dulbecco's Modified Eagle's Medium (Sigma-Aldrich Co., St. Louis, MO, USA) with 10% fetal bovine serum (Biowest, Nuaille, France) containing penicillin (100 U/ml) and streptom ycin (100 μ g/ml (Life Technologies, Inc. BLR, Grand Island, NY, USA). The cells were grown in 75-cm 2 tissue culture flasks in a humidified 5% CO 2 , 95% air atmosphere in a CO 2 incubator at 37 °C. Normoglycemic and hyperglycemic mice were injected subcutaneously under the fourth nipple of the mammary gland, with 1 × 10 3 4T1cells in 50 μl of isotonic solution. The area of injection was previously shaved and disinfected [19] . Mice were sacrificed 7 weeks (49 days) later of the induction of tumors. Tumors were weighed and groups of 2 or 3 tumors were pooled and processed separately. Survival was recorded for 8 weeks following tumor induction.

Viedma-Rodríguez R., Martínez-Hernández M.G., Martínez-Torres D.I, & Baiza-Gutman L.A. (2020). Epithelial Mesenchymal Transition and Progression of Breast Cancer Promoted by Diabetes Mellitus in Mice Are Associated with Increased Expression of Glycolytic and Proteolytic Enzymes. Hormones & cancer, 11(3-4).

+ Open protocol

+ Expand

Breast Cancer Cell Line Cytotoxicity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gemcitabine hydrochloride, MW 299.66, β-glucan, (hydroxypropyl)methyl cellulose (HPMC), ethylcellulose (with 48% ethoxy content) (EC), acetic acid, 3-(4, 5-dimethyl-thiazol-2-yl)-2, and 5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louise, MO, USA). Methylcellulose LR was purchased from Chem-Supply (Gillman, South Australia). Chitosan (MW 150,000, DD 70%), was purchased from Comwin Fine Chemicals Co. (Changzhou, China). Methylcellulose (MC) was purchased from ICN Biomedical, Inc. (Santa Ana, CA, USA). Polypropylene glycol was purchased from Midwest Pharmaceutics (Hawke’s Bay, New Zealand). Dimethyl sulfoxide (DMSO) was purchased from Labpartner (Shanghai, China). The 4T1breast cancer cell line was purchased from the American Type Culture Collection (ATCC; Rockville, MD, USA). The Roswell Park Memorial Institute (RPMI) medium 1640 basic was purchased from Gibco (Grand Island, NE, USA), and fetal calf serum was purchased from Hyclone (Logan, UT, USA). Penicillin–streptomycin–glutamine and nonessential amino acids were purchased from Life Technology (Grand Island, NY, USA). All other chemicals used were of reagent grade. Milli-Q water was obtained through reverse osmosis using a Millipak^® system (Millipore, Burlington, MA, USA, 0.22 µm).

Chen G., Liu Y., Svirskis D., Li H., Ying M., Lu W, & Wen J. (2024). Cryo-Milled β-Glucan Nanoparticles for Oral Drug Delivery. Pharmaceutics, 16(4), 546.

+ Open protocol

+ Expand

Culturing 4T1 Breast Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 4T1 breast cancer cell line was obtained from the American Type Culture Collection. Cells were cultured in RPMI-1640 medium (Sigma-Aldrich, cat # R5886) containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 2 mM L-glutamine and 1% pyruvate at 37 • C in a humidified incubator with 5% CO 2 . Cells were regularly checked for Mycoplasma contamination.

Schwarcz S., Nyerges P., Bíró T.I., Janka E., Bai P, & Mikó E. (2024). Cytostatic Bacterial Metabolites Interfere with 5-Fluorouracil, Doxorubicin and Paclitaxel Efficiency in 4T1 Breast Cancer Cells. Molecules (Basel, Switzerland), 29(13).

+ Open protocol

+ Expand

Characterization of Murine Breast Cancer Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The AT-3 tumor cell line was derived from PyMT-MMTV transgenic mice (33 (link)). The 4T1 breast cancer cell line was purchased from the American Type Culture Collection (ATCC), authenticated at ATCC and maintained. AT-3 cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Sigma), 0.5% penicillin/streptomycin (Gibco), 2 mM L-glutamine (Gibco), 1% NEAA (Gibco), and 55 μM 2-mercaptoethanol (Gibco). 4T1 cells were cultured in RPMI (Gibco) supplemented with 10% FBS, 0.5% penicillin/streptomycin, 2 mM L-glutamine, 1% NEAA, and 55 μM 2-mercaptoethanol. These cell lines were authenticated by morphology, phenotype and growth, and routinely screened for Mycoplasma, and were maintained at 37°C in a humidified 5% (4T1) or 7% (AT-3) CO₂ atmosphere.

Patel A., Oba T., Kajihara R., Yokoi T., Abrams S.I, & Ito F. (2021). Multimodal intralesional therapy for reshaping the myeloid compartment of tumors resistant to anti-PD-L1 therapy via IRF8. Journal of immunology (Baltimore, Md. : 1950), 207(5), 1298-1309.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!