Ocm 1

HEK293T cells (ACS-4500) were purchased from the American Type Culture Collection (ATCC). Thyroid cancer cell lines K1, BCPAP, TPC-1, KTC-1, 8505 C, Cal-62, and normal human thyroid cell line Nthy-ori 3-1 (Nthy) were obtained from Tianjin Medical University Cancer Institute and Hospital with STR profiles. Human melanoma cell lines A375, SK-MEL-28, A2058, and mouse melanoma cells B16-F10 were generously provided by Dr Lizhi Hu from Tianjin Medical University. Thyroid cancer cell line KHM-5M (3101HUMSCSP549), human uveal melanoma cell lines OCM-1, MUB-2B, human breast cancer cell line MCF-7 (1101HUM-PUMC000013) and human lung adenocarcinoma cell line A549 (1101HUM-PUMC000002) were obtained from Cell Resource Center, Peking Union Medical College (PCRC). HEK293T, A375, TPC-1, K1, OCM-1, B16-F10 cell lines were maintained in DMEM medium supplemented with 10% fetal bovine serum (FBS). Nthy, 8505 C, BCPAP, MUB-2B, Cal-62, KTC, KHM-5M were maintained in RPMI 1640 medium supplemented with 10% FBS. Usp15^+/+ and Usp15^-/- MEFs cells were isolated from E13.5 embryos of mice and cultured in DMEM supplemented with 10% FBS and 100 μg/mL streptomycin. All the cells were cultured in a humidified incubator equilibrated with 5% CO₂ at 37 °C.