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A375 human metastatic melanoma cell line

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The A375 human metastatic melanoma cell line is a standardized research tool derived from a malignant skin cancer. It is widely used in the study of melanoma biology and the development of related therapeutic approaches.

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A375 cells (54 citations) A375 cell line (32 citations) A375 melanoma (13 citations) A375 melanoma cell line (8 citations) A375 human melanoma (5 citations)

2 protocols using a375 human metastatic melanoma cell line

1

Melanoma Cell Lines for Research

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The human radial growth melanoma cell line WM1552c and the human vertical growth melanoma cell line WM793b were provided by Dr. M. Herlyn (Wistar Institute, Philadelphia, PA) and cultured as previously described [22 ]. The human metastatic melanoma cell line MEL 39 was a gift from Soldano Ferrone (Harvard Medical School, Boston, MA) and cultured as previously described [23 (link)]. The A375 human metastatic melanoma cell line was obtained from American Type Cell Culture Collection (ATCC, Manassas, VA).
Martin del Campo S.E., Latchana N., Levine K.M., Grignol V.P., Fairchild E.T., Jaime-Ramirez A.C., Dao T.V., Karpa V.I., Carson M., Ganju A., Chan A.N, & Carson III W.E. (2015). MiR-21 Enhances Melanoma Invasiveness via Inhibition of Tissue Inhibitor of Metalloproteinases 3 Expression: In Vivo Effects of MiR-21 Inhibitor. PLoS ONE, 10(1), e0115919.
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2

Metastatic Melanoma Cell Line and Tissue Acquisition

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The A375 human metastatic melanoma cell line was obtained from American Type Cell Culture Collection (ATCC, Manassas, VA). Melanoma tumor tissue samples were obtained from the OSU Wexner Medical Center. All patients provided written informed consent to store and use their tissue for discovery studies according to OSU institutional guidelines under protocols approved by the OSU Institutional Review Board.
Duggan M.C., Regan K., Olaverria Salavaggione G.N., Howard J.H., Stiff A.R., Sabella J., Latchana N., Markowitz J., Gru A., Tridandapani S., Eisfeld A.K., de la Chapelle A, & Carson WE I.I.I. (2019). NRAS mRNA is differentially spliced to give five distinct isoforms: implications for melanoma therapy. Melanoma research, 29(5), 491-500.
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