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Ferritin from horse spleen

Manufactured by Merck Group
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Sourced in China

Ferritin from horse spleen is a laboratory product offered by Merck Group. It is a protein complex that stores and releases iron in cells. The core function of this product is to provide a source of ferritin, a ubiquitous intracellular protein that plays a crucial role in iron homeostasis.

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Lab products found in correlation

Molecular weight cutoff spin filters, by Merck Group (2 mentions) Human 20s proteasome, by Enzo Life Sciences (2 mentions) Rabbit pyruvate kinase, by Roche (2 mentions) 2 2 bipyridine, by Merck Group (1 mentions) Bovine serum protein bsa, by Merck Group (1 mentions) Mops buffer, by Merck Group (1 mentions) Acetonitrile, by Sinopharm (1 mentions)

Spelling variants of this product

Ferritin (41 citations) Horse spleen ferritin (11 citations)

3 protocols using ferritin from horse spleen

1

Extraction and Characterization of Blueberry Anthocyanins

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The blueberry anthocyanins were extracted from Rabbiteye blueberries (Lishui Town, Nanjing, China) [53 (link)] and stored at −18 °C at the Institute of Agricultural Products Processing, Chinese Academy of Agricultural Sciences. High-performance liquid chromatography (HPLC) solvents potassium chloride and acetonitrile were acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) and TEDIA (Fairfield, OH, USA), respectively. Ferritin (from horse spleen), MOPS buffer, 2,2-bipyridine, and bovine serum protein (BSA) were procured from Sigma-Aldrich (Shanghai, China). The bicinchoninic acid (BCA) and protein-assay kits were purchased from Beijing Solarbio Company (Beijing, China). The reagents used in the experiment are all of analytical grade.
Huang W., Zhao X., Chai Z., Herrera-Balandrano D.D., Li B., Yang Y., Lu S, & Tu Z. (2023). Improving Blueberry Anthocyanins’ Stability Using a Ferritin Nanocarrier. Molecules, 28(15), 5844.
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2

Purification and Characterization of Enzymes

Cited 1 time
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Recombinant toyocamycin nitrile hydratase was expressed and purified as described previously24 (link). Rabbit pyruvate kinase was purchased from Roche; the phosphoglycerate mutase 2 was discovered as a contaminant in that sample. Glyceraldehyde 3-phosphate dehydrogenase was isolated from HeLa S3 cells as described previously25 . Ferritin from horse spleen was purchased from Sigma Aldrich. Finally, human 20S proteasome was purchased from Enzo Life Sciences. All samples were desalted prior to analysis by buffer exchanging with molecular weight cutoff spin filters (Millipore) into 150 mM ammonium acetate at pH 7.
Skinner O.S., Havugimana P.C., Haverland N.A., Fornelli L., Early B.P., Greer J.B., Fellers R.T., Durbin K.R., Do Vale L.H., Melani R.D., Seckler H.S., Nelp M.T., Belov M.E., Horning S.R., Makarov A.A., LeDuc R.D., Bandarian V., Compton P.D, & Kelleher N.L. (2016). An informatic framework for decoding protein complexes by top-down mass spectrometry. Nature methods, 13(3), 237-240.
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3

Purification and Characterization of Enzymes

Cited 1 time
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Recombinant toyocamycin nitrile hydratase was expressed and purified as described previously24 (link). Rabbit pyruvate kinase was purchased from Roche; the phosphoglycerate mutase 2 was discovered as a contaminant in that sample. Glyceraldehyde 3-phosphate dehydrogenase was isolated from HeLa S3 cells as described previously25 . Ferritin from horse spleen was purchased from Sigma Aldrich. Finally, human 20S proteasome was purchased from Enzo Life Sciences. All samples were desalted prior to analysis by buffer exchanging with molecular weight cutoff spin filters (Millipore) into 150 mM ammonium acetate at pH 7.
Skinner O.S., Havugimana P.C., Haverland N.A., Fornelli L., Early B.P., Greer J.B., Fellers R.T., Durbin K.R., Do Vale L.H., Melani R.D., Seckler H.S., Nelp M.T., Belov M.E., Horning S.R., Makarov A.A., LeDuc R.D., Bandarian V., Compton P.D, & Kelleher N.L. (2016). An informatic framework for decoding protein complexes by top-down mass spectrometry. Nature methods, 13(3), 237-240.
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