Penicillin streptoycin

Manufactured by Thermo Fisher Scientific

Sourced in United States, Japan

Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It provides protection against bacterial contamination by inhibiting the growth of both gram-positive and gram-negative bacteria.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hsc t6, by iCell Bioscience (1 mentions) Aml12 cells, by iCell Bioscience (1 mentions) Dibutyryl cyclic amp dbcamp, by Merck Group (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) 3 isobutyl 1 methylxanthine ibmx, by Merck Group (1 mentions) Human recombinant basic fibroblast growth factor bfgf, by R&D Systems (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Neurobasal medium, by Thermo Fisher Scientific (1 mentions) Human epidermal growth factor (hegf), by Merck Group (1 mentions)

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Penicillin (24825 citations)

2 protocols using penicillin streptoycin

Culturing Hepatic Stellate and Hepatocyte Cell Lines

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The hepatic stellate cell line, HSC-T6 (iCell Bioscience Inc, Shanghai, China) were cultured in DMEM medium (Cat.C11995500BT, Gibco, USA) supplemented with 1% penicillin/streptoycin (Cat. 15410, Gibco, BRL, USA) and 10% fetal bovine serum (FBS, Cat. P30-3302, PAN-Biotech, Germany). HSC-T6 cells were cultured at 37 ℃ in an incubator containing 5% CO₂.
The hepatocyte line, AML12 cells (iCell Bioscience Inc, Shanghai, China) were culured in DME/F-12 medium (Cat.SH30023.01, HyClone, USA) supplemented with 1% penicillin/streptoycin and 10% fetal bovine serum. AML12 cells were cultured at 37 ℃ in an incubator containing 5% CO₂.

Song X., Shi J., Liu J., Liu Y., Yu Y., Qiu Y., Cao Z., Pan Y., Yuan X., Chu Y, & Wu D. (2022). Recombinant truncated latency-associated peptide alleviates liver fibrosis in vitro and in vivo via inhibition of TGF-β/Smad pathway. Molecular Medicine, 28, 80.

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Neuronal Differentiation of hMSC

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Human bone marrow derived mesenchymal stem cell (hMSC) line (UE7T-13 cells, no. RBRC-RCB2161; RIKEN, Japan) [28 (link)–32 (link)], Growing and expansion media of hMSCs: Dulbecco’s Medified Eagle’s Medium (DMEM; Gibco) with 2mM _L-Glutamine, Fetal bovine serum (FBS; Gibco), Penicillin/Streptoycin (Gibco). Neuronal induction: dibutyryl cyclic AMP (dbcAMP; Sigma), 3-isobutyl-1-methylxanthine (IBMX; Sigma), human epidermal growth factor (hEGF; Sigma), recombinant human basic fibroblast growth factor (bFGF; R&D systems), fibroblast growth factor-8 (FGF-8; Pepro Tech), recombinant human brain-derived neurotrophic factor (BDNF; R&D systems), and nerve growth factor (NGF), Neurobasal medium (Gibco) supplemented with 2% B27 supplement (Gibco), 2 mM l-glutamine (Gibco).

Karakaş N., Bay S., Türkel N., Öztunç N., Öncül M., Bilgen H., Shah K., Şahin F, & Öztürk G. (2020). Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS ONE, 15(5), e0228510.

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