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Pimozide

Manufactured by Bio-Techne
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Sourced in Germany

Pimozide is a laboratory product manufactured by Bio-Techne. It is a dopamine receptor antagonist that can be used in research applications.

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Lab products found in correlation

Trametinib, by LC Laboratories (3 mentions) Ruxolitinib, by LC Laboratories (2 mentions) Imatinib, by LC Laboratories (2 mentions) Ponatinib, by LC Laboratories (2 mentions) Hbx 41 108, by Bio-Techne (2 mentions) Ubiquitin monomer, by Merck Group (2 mentions) Maldi tof ms materials, by Bruker (2 mentions) Degrasyn wp1130, by Bio-Techne (2 mentions) L434078, by Merck Group (2 mentions) Dithiothreitol (dtt), by Merck Group (2 mentions) Pr 619, by Merck Group (2 mentions) P22077, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Tofacitinib, by Apexbio (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Chlorpromazine, by Merck Group (1 mentions) Rolipram, by Merck Group (1 mentions) Reboxetine, by Bio-Techne (1 mentions) Phenelzine, by Merck Group (1 mentions) Clomipramine, by Merck Group (1 mentions)

7 protocols using pimozide

1

Inhibitor-based Signaling Pathway Analysis

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Imatinib was purchased from LC Laboratories. Stock solutions were prepared in sterile water at 10 mmol/L and stored at −20°C. Small molecule inhibitor of DUSP6 (2-benzylidene-3-(cyclohexylamino)-1-lndanone hydrochloride; BCI) and the ABL1 activator DPH were purchased from Sigma-Aldrich. BCI-215 was a gift from Dr. Andreas Vogt. The MEK inhibitor (trametinib), the JAK inhibitor (ruxolitinib) and ponatinib were purchased from LC Laboratories. Pimozide (STAT5 inhibitor) was purchased from TOCRIS. Stock solutions were prepared in DMSO at 10 mmol/L and stored at −20°C. For in vivo experiments, compounds were dissolved in NMP:PEG300 (1:9). Ph-like ALL cells (Fig 2e) were treated with ruxolitinib for 20 hours and then processed for Western blotting.
Chan L.N., Murakami M.A., Robinson M.E., Caeser R., Sadras T., Lee J., Cosgun K.N., Kume K., Khairnar V., Xiao G., Ahmed M.A., Aghania E., Deb G., Hurtz C., Shojaee S., Hong C., Pölönen P., Nix M.A., Chen Z., Chen C.W., Chen J., Vogt A., Heinäniemi M., Lohi O., Wiita A.P., Izraeli S., Geng H., Weinstock D.M, & Müschen M. (2020). Signaling input from divergent pathways subverts B-cell transformation. Nature, 583(7818), 845-851.
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2

Diubiquitin Topoisomer Characterization

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Ubiquitin monomer, BSA, Tris and DTT were purchased from Sigma-Aldrich. Diubiquitin topoisomers (M1, K6, K11, K27, K29, K33, K48 and K63-linked) were purchased from Boston Biochem (Boston, MA), additional K27 diubiquitin was produced in-house57 (link)58 , whereas all MALDI-TOF MS materials (targets, matrix and protein calibration mixture) were purchased from Bruker Daltonics (Bremen, Germany). PR-619 and P22077 (Calbiochem/Merck, Darmstadt, Germany) as well as HBX 41,108, pimozide and degrasyn/WP1130 (Tocris Bioscience, Bristol, UK) and L434078 (Sigma-Aldrich, St Louis, MO, USA) were purchased commercially. Febuxostat, SJB3-019A, compound 16, NSC 697923 and BAY 11-7082 were synthesized (Supplementary Table 3).
Ritorto M.S., Ewan R., Perez-Oliva A.B., Knebel A., Buhrlage S.J., Wightman M., Kelly S.M., Wood N.T., Virdee S., Gray N.S., Morrice N.A., Alessi D.R, & Trost M. (2014). Screening of DUB activity and specificity by MALDI-TOF mass spectrometry. Nature Communications, 5, 4763.
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3

Cytokine and Drug Treatment of BAL Cells

Cited 1 time
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BAL cells were pelleted, washed and cultured in the medium (RPMI 1640 plus 10% FBS) alone or with cytokines (as described for PBMCs in the previous section) with vehicle (0.1% DMSO) or Dexamethasone (10−7 M, from Sigma-Aldrich, Inc. St. Louis, MO). In select experiments Trametinib (10 μM) from LC Laboratories, Tofacitinib (0.2 μM) from ApexBio, or Pimozide (5 μM) from Tocris Bioscience was added to the culture. BAL cells were cultured for 3 days until otherwise stated. Monensin (2 μM) was added to all cultures 4 hr before conclusion. Following culture cells were pelleted and then stained for flow cytometry as described previously (7 (link), 15 (link)).
Liu S., Verma M., Michalec L., Liu W., Sripada A., Rollins D., Good J., Ito Y., Chu H., Gorska M.M., Martin R.J, & Alam R. (2017). Steroid Resistance of Airway Type 2 Innate Lymphoid Cells (ILC2s) from Severe Asthma: The Role of Thymic Stromal cell Lymphopoietin (TSLP). The Journal of allergy and clinical immunology, 141(1), 257-268.e6.
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4

Evaluation of Antidepressant Compounds

Cited 1 time
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Fluoxetine (#H6995, Bosche Scientific), citalopram (#C505000, Toronto Research Chemicals), paroxetine (#2141, Tocris), fluvoxamine (#1033), venlafaxine (#2917, Tocris), reboxetine (#1982, Tocris), imipramine (#I7379-5G, Sigma–Aldrich), clomipramine (#C7291, Sigma–Aldrich), desipramine (#3067, Tocris), phenelzine (#P6777, Sigma–Aldrich), rolipram (#R6520, Sigma–Aldrich), ketamine (#3131, Tocris), 2R, 6R-Hydroxynorketamine and 2S, 6S-Hydroxynorketamine (#6094 and #6095, respectively, Tocris), carbamazepine (#4098, Tocris), chlorpromazine (#C8138, Sigma–Aldrich), flupenthixol (#4057, Tocris), and pimozide (#0937, Tocris) were investigated in this study. The compounds were dissolved in dimethyl sulfoxide (DMSO) that was also used as a vehicle in all the experiments. Concentrations tested (Table 1) were selected based on earlier in vitro studies from our research group (Fred et al., 2019 (link); Casarotto et al., 2021 (link)) and other laboratories (Johansen et al., 2015 (link)).
Fred S.M., Kuivanen S., Ugurlu H., Casarotto P.C., Levanov L., Saksela K., Vapalahti O, & Castrén E. (2022). Antidepressant and Antipsychotic Drugs Reduce Viral Infection by SARS-CoV-2 and Fluoxetine Shows Antiviral Activity Against the Novel Variants in vitro. Frontiers in Pharmacology, 12, 755600.
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5

Quantitative Analysis of Diubiquitin Linkages

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Ubiquitin monomer, Bovine Serum Albumin (BSA), Tris and DTT were purchased from Sigma Aldrich. Diubiquitin topoisomers (M1, K6, K11, K27, K29, K33, K48 and K63-linked) were purchased from Boston Biochem (Boston, MA), additional K27 diubiquitin was produced in-house57 (link), 58 (link), whereas all MALDI-TOF MS materials (targets, matrix and protein calibration mixture) were purchased from Bruker Daltonics (Bremen, Germany). PR-619 and P22077 (Calbiochem/Merck, Darmstadt, Germany) as well as HBX 41,108, Pimozide and Degrasyn/WP1130 (Tocris Bioscience, Bristol, UK) and L434078 (Sigma-Aldrich, St. Louis, MO, USA) were purchased commercially. Febuxostat, SJB3-019A, Compound 16, NSC 697923 and BAY 11-7082 were synthesized (Supplementary Table 3).
Ritorto M.S., Ewan R., Perez-Oliva A.B., Knebel A., Buhrlage S.J., Wightman M., Kelly S.M., Wood N.T., Virdee S., Gray N.S., Morrice N.A., Alessi D.R, & Trost M. (2014). Screening of DUB activity and specificity by MALDI-TOF mass spectrometry. Nature Communications, 5, 4763.
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6

Small Molecule Screening Compounds

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All-trans retinal, fibronectin, amitriptyline hydrochloride, nicardipine, amlodipine besylate, diltiazem hydrochloride, and verapamil were from Sigma Aldrich (St. Louis, MO). Mibefradil was from Alomone Labs (Jerusalem, Israel). Pimozide was from Tocris Bioscience (Bristol, UK).
Agus V., Flak T.A., Picardi P., Pizzi S., Rutigliano L., Cainarca S., Redaelli L., Rolland J.F, & Scarabottolo L. (2021). Parallel All-Optical Assay to Study Use-Dependent Functioning of Voltage-Gated Ion Channels in a Miniaturized Format. SLAS discovery : advancing life sciences R & D, 26(3).
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7

Inhibitor-based Signaling Pathway Analysis

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Imatinib was purchased from LC Laboratories. Stock solutions were prepared in sterile water at 10 mmol/L and stored at −20°C. Small molecule inhibitor of DUSP6 (2-benzylidene-3-(cyclohexylamino)-1-lndanone hydrochloride; BCI) and the ABL1 activator DPH were purchased from Sigma-Aldrich. BCI-215 was a gift from Dr. Andreas Vogt. The MEK inhibitor (trametinib), the JAK inhibitor (ruxolitinib) and ponatinib were purchased from LC Laboratories. Pimozide (STAT5 inhibitor) was purchased from TOCRIS. Stock solutions were prepared in DMSO at 10 mmol/L and stored at −20°C. For in vivo experiments, compounds were dissolved in NMP:PEG300 (1:9). Ph-like ALL cells (Fig 2e) were treated with ruxolitinib for 20 hours and then processed for Western blotting.
Chan L.N., Murakami M.A., Robinson M.E., Caeser R., Sadras T., Lee J., Cosgun K.N., Kume K., Khairnar V., Xiao G., Ahmed M.A., Aghania E., Deb G., Hurtz C., Shojaee S., Hong C., Pölönen P., Nix M.A., Chen Z., Chen C.W., Chen J., Vogt A., Heinäniemi M., Lohi O., Wiita A.P., Izraeli S., Geng H., Weinstock D.M, & Müschen M. (2020). Signaling input from divergent pathways subverts B-cell transformation. Nature, 583(7818), 845-851.
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