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2 protocols using ascorbic acid

1

Silane-based Functionalization for Biosensors

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Tetraethyl orthosilicate (TEOS, 99.999%), 3-(trimethoxysilyl)-1-propanamine (APTMS, 97%), (3-mercaptopropyl)trimethoxysilane (MPTMS, 95%), TSPP (≥95.0%), TPEN (≥98.0%), ZnCl2 (99.99%), and ammonia solution (99.99%) were supplied by Sigma-Aldrich. Ascorbic acid (99%) was purchased from J&K. SOCl2 (99.5%), imidazole (99.5%), NaNO2 (99%), FeCl3 (99.9%), and chitosan (≥95% deacetylated) were obtained from Aladdin. Phosphoric acid (analytical reagent), nitric acid (analytical reagent), and cellulose paper were purchased from Wanqing Chemical (Nanjing, China). Pure water (18.2 MΩ cm−1) was obtained from a Pall Cascade AN system.
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2

Synthesis and Characterization of Gold Nanorods

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Gold(III) chloride trihydrate (HAuCl 4 ·3H 2 O ≥99.9%) was purchased from J&K Scientific Ltd. Ascorbic acid (AA ≥99.0%), silver nitrate (>99%), sodium borohydride (NaBH 4 , 98%), cetyltrimethylammonium bromide (CTAB ≥99%) were obtained from Sigma-Aldrich. Amplex Red was obtained from Thermo Fisher Scientific. H 2 O 2 was obtained from Sinopharm Chemical Reagent Co., Ltd. Au NRs were synthesized according to a seed-mediated growth approach. 1
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