Standard chemicals, 2-thenoyltrifluoroacetone (TTFA; #T27006 ), rotenone (#R8875), sodium azide BioXtra (#S8032), antimycin A (#A8674), dimethyl malonate (#04011), d -α tocopherol succinate (#T3126), 2,6-dichloroindophenol sodium salt hydrate (DCPIP; #D1878), phenazine methosulphate (PMS; #P9625), MTT (#M5655), acetyl CoA (#A2181), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB; #D218200), bovine serum albumin (#A6003), doxycycline hyclate (#D9891), 3-Nitropropionic acid (#N5636) and oxaloacetic acid (#O4126) were purchased from Sigma-Aldrich. Carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone (FCCP; #BML-CM120-0010) was purchased from Enzo Life Sciences. Venetoclax (#2253-1) and IACS-010759 (#B2231-1) were purchased from Biovision Inc. Potassium phosphate monobasic (KH2PO4; #BP362-500) and ethylenediamine tetra acetic acid (EDTA; #BP118-500) were purchased from Fisher Scientific. Decylubiquinone was purchased from Abcam (#ab145233). Oligomycin was purchased from Merck Millipore (#495455). Triton X-100 (0694) was purchased from Amresco. Polybrene (#TR-1003-G) was purchased from Millipore. Puromycin (#631306) was purchased from Clontech.
2 thenoyltrifluoroacetone
Manufactured by Merck Group
Sourced in United States
2-thenoyltrifluoroacetone is a chemical compound used as a laboratory reagent. It is a colorless liquid with a characteristic odor. The compound has a molecular formula of C₇H₅F₃OS and a molar mass of 210.15 g/mol. 2-thenoyltrifluoroacetone is commonly used in various analytical and synthetic applications within the scientific community.
Automatically generated - may contain errors
Lab products found in correlation
Antimycin a, by Merck Group (4 mentions)
Rotenone, by Merck Group (3 mentions)
Potassium phosphate monobasic kh2po4, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Triton x 100, by Avantor (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Polybrene, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid edta, by Thermo Fisher Scientific (1 mentions)
Oligomycin, by Merck Group (1 mentions)
Puromycin, by Takara Bio (1 mentions)
Doxycycline hyclate, by Merck Group (1 mentions)
Acetyl coa, by Merck Group (1 mentions)
Oxaloacetic acid, by Merck Group (1 mentions)
Venetoclax, by Abcam (1 mentions)
Dimethyl malonate, by Merck Group (1 mentions)
D α tocopherol succinate, by Merck Group (1 mentions)
3 nitropropionic acid, by Merck Group (1 mentions)
Sodium azide bioxtra, by Merck Group (1 mentions)
2 6 dichloroindophenol sodium salt hydrate, by Merck Group (1 mentions)
Phenazine methosulphate pms, by Merck Group (1 mentions)
7 protocols using 2 thenoyltrifluoroacetone
1
Measurement of Mitochondrial Function
2
Mitochondrial Electron Transport Chain Inhibitors
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T-2307 was synthesized by FUJIFILM Toyama Chemical Co., Ltd. (Tokyo, Japan). Antimycin A, rotenone, 2-thenoyltrifluoroacetone (TTFA), and oligomycin A were purchased from Sigma-Aldrich (St. Louis, MO, USA). KCN and CCCP were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Malonic acid was purchased from Tokyo Chemical Industry (Tokyo, Japan).
3
Synthesis and Characterization of Europium-based Luminescent Polymer Particles
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Europium (III) chloride hexahydrate (EuCl3·6H2O), 2-thenoyltrifluoroacetone (TTA), 1,10-phenanthroline (Phen), N,N-dimethylformamide (DMF), succinic anhydride, styrene (St), methyl methacrylate (MMA), potassium persulfate (KPS), p-toluene sulfonyl chloride (99%), polyvinylpyrrolidone (K30, Wt. 40,000), Sodium bicarbonate (98%), Sodium dodecyl sulfate (99%), Dichloromethane, Terahydrofuran, and D-(+)-glucose were purchased from Sigma-Aldrich (Shanghai, China). Sodium phosphate dibasic, sodium phosphate monobasic monohydrate, bovine serum albumin (BSA), dimethyl sulphoxide (DMSO), and Tween20 were purchased from Shanghai Sangon Ltd. (Shanghai, China). Gibco® newborn bovine serum was purchased from Thermo Fisher Scientiflc, Inc. (Waltham, MA, USA). Goat anti-mouse IgG antibody was purchased from Arista Biologicals, Inc. (Allentown, PA, USA). styrene and methyl methacrylate were washed using 10% sodium hydroxide solution and deionized water three times before use to remove the inhibitor.
4
CRISPR/CAS9 Silencing Assay in Cancer Cells
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143B (ATCC® CRL-8303™) and A549 (ATCC® CCL-185™) cells were cultured in DMEM supplemented with 10% FBS, 1% penicillin and streptomycin at 37 °C, 5% CO2. siRNA expression vector pSilencer™ Puro Expression Vectors kit (Applied Biosystems,Life technologies), the CRISPR/CAS 9 plasmid pSpCas9(BB)-2A-GFP was from Addgene and TurboFect transfection reagent from Thermo Scientific. The kits: The quick ligation kit (NEB); RNeasy mini kit (Qiagen); High Capacity cDNA Reverse Transcription Kit (Applied Biosystems); KAPA SYBR® FAST qPCR Kit (Kapa Biosystems); Cell Proliferation Kit II (XTT) (Sigma-Aldrich); Reagents: L-glutamine solution, dialyzed FBS, glucose, galactose, glycine, serine, L-aspartic acid sodium salt monohydrate, OAA, phosphoenolpyruvate (PEP), β-nicotinamide adenine dinucleotide sodium salt and aminooxyacetate (AOA), Antimycin A and 2-thenoyltrifluoroacetone were from Sigma. The glucose and pyruvate-free and glutamine free-DMEM were from Thermo Fisher Scientific.
5
Mitochondrial Electron Transport Inhibitors
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Rotenone, 2-thenoyltrifluoroacetone, antimycin A, myxothiazol, potassium cyanide, 3,4-dehydro-l -proline, carboxin, methyl-succinate, l -proline, coenzyme Q1, doxycycline, N-acetyl cysteine, and o-aminobenzaldehyde were purchased from Sigma Aldrich, St. Louis, MO. Atpenin A5 was purchased from Santa Cruz Biotechnology, Dallas, Texas.
6
Ferroptosis-Related Biochemical Assays
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Erastin, GSH, Ferrostatin-1 were purchased from Selleck Chemicals (Houston, TX). DFO, 2-Thenoyltrifluoroacetone (TTFA), Lonidamine (LND), diethyl butylmalonate (DBM), Antimycin A, 2’,7’-dichlorofluore scindiacetate (DCF-DA) were purchased from Sigma-Aldrich (St. Louis, USA). Cell Counting Kit-8 (CCK-8) kit was obtained from MultiSciences (Hangzhou, China). Lipid Peroxidation Fluorescent Probe (C11 BODIPY 581/591), mitoSOX fluorescence probes were purchased from Thermo Fisher Scientific (Waltham, MA).
7
Synthesis of Nanocrystal Materials
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Phenyl ether (99%), oleylamine (470%), oleic acid (90%), iron(III) acetylacetonate (99%), 1,2-fexadecanediol (90%), n-hexane (97%), cyclohexane (99.5%), Igepal s CO-520, tetraethylorthosilicate (TEOS, 499%), 2-thenoyltrifluoroacetone (99%), dibenzoylmethane (98%) ammonia solution (25-28 wt%), ethanol absolute (ACS grade), N,Ndimethylformamide -DMF (ACS grade), tetrahydrofuran -THF anhydrous, were purchased from Sigma Aldrich. The water used in all experiments was ultrapure. All other chemicals were used as received without further purification.
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