PCa cell lines (PC3, DU145, 22RV1 and LNCaP) were purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China). All the cell lines were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Jinuo Biomedical Technology, Co., Ltd., Hangzhou, China), in a 5% CO2 atmosphere at 37°C. Treatment with foretinib (Shanghai Selleck Chemicals, Co., Ltd., Shanghai, China), recombinant human HGF (Thermal Tech) and recombinant human MSP (R&D Systems, Minneapolis, MN, USA) was performed according to the manufacturers instructions.
Recombinant human msp
Manufactured by R&D Systems
Sourced in United States
Recombinant human MSP is a recombinant protein produced in E. coli cells. It represents the full-length sequence of the human Macrophage Stimulating Protein (MSP) and can be used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Lncap, by National Collection of Authenticated Cell Cultures (1 mentions)
Du145, by National Collection of Authenticated Cell Cultures (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
P tyr 100, by Cell Signaling Technology (1 mentions)
Ox ldl, by R&D Systems (1 mentions)
Palmitic acid, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by R&D Systems (1 mentions)
Bms 777607, by Selleck Chemicals (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Foetal bovine serum (fbs), by Merck Group (1 mentions)
5 protocols using recombinant human msp
1
Prostate Cancer Cell Line Maintenance
2
Investigating Gastric Cancer Cell Line MGC-803
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Human gastric cancer line MGC-803 was obtained from the State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine. PrimeScript™ RT reagent kit and SYBR Premix Ex Taq™ reagent kits were purchased from Takara Biotechnology (Dalian Co., Ltd, China). Lipofectamine™ 2000 and the cell cycle reagent kit were purchased from Invitrogen (Life Technologies, USA). Hygromycin B and c-Met/RON small molecular inhibitor (Compound I) were obtained from Calbiochem. Recombinant human MSP was purchased from R&D systems. Cell counting kit 8 (CCK-8) was purchased from Dojindo Molecular Technologies (Rockville, USA). Antibodies against RON (β-chain) were purchased from Santa Cruz Biotechnology (USA). The recombinant plasmid pcDNA3.1-RONΔ160 and blank plasmid pcDNA3.1 were obtained from Sangon Biotech Co., Ltd (China). Female Balb/c athymic nude mice (Nu/Nu) aged 6-weeks were obtained from the B&K Universal Group and were kept in temperature-controlled rooms with a 12-h alternating light-dark cycle.
3
Protein Signaling Pathway Analysis
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Recombinant human MSP was from R&D Systems (Minneapolis, MN, USA). The rabbit IgG antibodies specific to phospho-PTEN (Ser380/Thr382/383), phpspho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204), phospho-(Ser/Thr) AKT, pan-AKT (C67E7), the rabbit mAb to p44/42 MAPK (Erk1/2)(137F5), the rabbit mAb to phospho-mTOR (Ser2448) (D9C2) and the mouse anti-phospho tyrosine mAb (p-tyr-100) were from the company Cell Signaling, Inc. (Beverly, MA, USA). The rabbit IgG antibodies specific to the extracellular domain of human RON (H160), the C-terminal of RON(C-20), and the mouse mAb specific to PTEN were from Santa Cruz Biotechnology(Santa Cruz, CA, USA). The mouse mAb against the RON α-chain was from BD Biosciences (San Jose, CA, USA). Tyrosine kinase assay kit (non-radioactive) was from Upstate (Lake Placid, NY). The normal rabbit or mouse IgG were from Beyotime biotechnology(Beyotime, Shanghai, China).
4
Recombinant Biomolecule Procurement Protocol
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Recombinant human MSP, LPS and oxLDL were purchased from R&D systems. Palmitic acid (PA) was purchased from Sigma-Aldrich (St Louis, MO, USA).
5
Isolation and Culture of Human Endometrial Epithelial Cells
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Isolation of primary human endometrial epithelial cells was undertaken as described in our previous paper.37 Then, cells were cultured in Dulbecco's Modified Eagle's medium: Nutrient Mixture F‐12 (DMEM/F 12; Keyi Biotechnology) containing penicillin (50 U/mL), streptomycin (50 μg/mL) and foetal bovine serum (FBS; 12% (v/v)), all of which were from Sigma‐Aldrich. The primary human endometrial epithelial cells we used were isolated from the control endometria in the proliferative phase and named ‘control endometrial epithelial cells’ (CEECs).
Cells were serum‐starved (0.5% FBS) for 24 hours before addition of BMS 777607 (Selleck Chemicals) or recombinant human MSP (R & D Systems).
Cells were serum‐starved (0.5% FBS) for 24 hours before addition of BMS 777607 (Selleck Chemicals) or recombinant human MSP (R & D Systems).
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