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1260 infinity quaternary pump vl

Manufactured by Agilent Technologies
Sourced in Italy

The 1260 Infinity Quaternary Pump VL is a versatile liquid chromatography pump capable of delivering four different solvents simultaneously. It is designed to provide precise and reliable solvent delivery for a variety of analytical applications.

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2 protocols using 1260 infinity quaternary pump vl

1

Decolorization of Dyes via HPLC

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The decolorization of malachite green, methylene blue and congo red dyes at different intervals were extracted with equal volume of dichloromethane. The extracts were evaporated at 40 °C in a rotary evaporator (Billy scientific with stuart thermostat water bath). The extracted residues were dissolved in methanol (HPLC grade) and used for HPLC analysis.
HPLC analysis was performed in an isocratic system (Agilent technologies 1260 Infinity Quaternary Pump VL) equipped with a UV detector. The separation was performed using C18 column (4.6 × 100 mm) with methanol:acetonitrile (1:1) as mobile phase at a flow rate of 1 mL/min.
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2

HPLC Analysis of EVE Encapsulated in NPs

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An HPLC method was in house-modified to quantify the amount of EVE encapsulated in NPs, and to study the release profile [82 (link),83 (link)]. The analyses were carried out on a Zorbax Eclipse® Plus C18 Chromatography Column, 4.6 mm × 150 mm, 5 μm heated at 55 °C. The mobile phase was prepared by mixing purified water, methanol, and acetonitrile (ratio 22:60:18); the flow rate was fixed at 1.5 mL/min; and the detection wavelength was fixed at 278 nm. All of the chromatographic experiments were carried out on an Agilent 1260 HPLC (Agilent Technologies, Milan, Italy) consisting of a pump (1260 Infinity Quaternary Pump VL), UV detector (Agilent 1260 Series UV-visible detectors, multi-wavelength detector), and manual injector (Agilent 1260 Infinity Manual Injector). The calibration curve, in a concentration range from 1 to 10 μg/mL, had a linearity of R2 = 0.9969, all of the measurements were ranged in the level of detection (LOD 0.30 μg/mL) and level of quantification (LOQ 1 μg/mL). The HPLC method showed a recovery of 98.4 ± 1.7%.
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