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Newborn bovine serum

Manufactured by Merck Group
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Sourced in United Kingdom, United States

Newborn bovine serum is a cell culture media supplement derived from the blood of newborn calves. It is a rich source of proteins, growth factors, and other nutrients that support the growth and proliferation of a wide range of cell types in vitro.

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Lab products found in correlation

Disodium edta, by Merck Group (1 mentions) Pokeweed mitogen, by Merck Group (1 mentions) Sac pansorbin cells, by Merck Group (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic reagent, by Thermo Fisher Scientific (1 mentions) Auranofin, by MedChemExpress (1 mentions) Palbociclib, by MedChemExpress (1 mentions) 1640 media, by Merck Group (1 mentions)

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Bovine serum (80 citations)

4 protocols using newborn bovine serum

1

Polyclonal B-cell Stimulation and Differentiation

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2×105 cells/well of isolated PBMCs were seeded into 96-well, cell culture treated plates (Greiner-One Bio, UK) and stimulated with 100 µl/well of a mixture of 1∶5000 Staphylococcus aureus Cowan 1 strain (SAC Pansorbin cells, Merck-Millipore, UK), 1.7 µg/ml CpG (BioScience Ltd, UK) and 83.33 ng/ml pokeweed mitogen (Sigma-Aldrich, UK). This combination induces polyclonal stimulation of B-cells and maximal proliferation of memory B-cells allowing differentiation of small antigen-specific populations into IgG-ASCs that may be detected by ELISpot [14] (link), [15] (link). Plates were incubated at 37°C, 5% CO2 and 95% humidity for 5–6 days. Harvested cells were washed in phosphate buffered saline with ethylenediaminetetraacetic acid (EDTA) di-sodium and 0.5% newborn bovine serum (Sigma-Aldrich, UK) and re-suspended in R10 medium to a concentration of 2×106 cells/ml.
Khatami A., Clutterbuck E.A., Thompson A.J., McKenna J.A., Pace D., Birks J., Snape M.D, & Pollard A.J. (2014). Evaluation of the Induction of Immune Memory following Infant Immunisation with Serogroup C Neisseria meningitidis Conjugate Vaccines – Exploratory Analyses within a Randomised Controlled Trial. PLoS ONE, 9(7), e101672.
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2

Mesothelioma Cell Lines and Antibody Analysis

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The mesothelioma cell lines used in this study (H2052, H2373, H2452, and H2052) and the control cell lines, Met5A (we note as Met5A AD) and 3T3, were obtained from either the ATCC (American Type Culture Collection) or in collaboration with Dr. Robert Kratzke (University of Minnesota). All mesothelioma cell lines lack functional p16INK4a. The cells were grown in RPMI-1640 medium (Gibco BRL, Grand Island, NY, USA) supplemented with 10% newborn bovine serum (Sigma, St. Louis, MO, USA), and 1x concentration of antibiotic/antimycotic reagent (Gibco BRL, Grand Island, NY, USA) at 37 °C and 5% CO2. The following antibodies were utilized (Actin, Bax, Bcl-xL, Bid, cdc2 (CDK1), CDK2, CDK4, CDK6, Cyclin A, Cyclin B1, Cyclin D1, Cyclin E1, FOXM1, Mcl-1, pRb (S780), Rb, Survivin, and XIAP (see Supplementary Materials for manufacturer and lot numbers). The CDK4/6 inhibitor, palbociclib, and auranofin were obtained from MedChemExpress (Monmouth Junction, NJ, USA).
Kratzke M., Scaria G., Porter S., Kren B, & Klein M.A. (2023). Inhibition of Mitochondrial Antioxidant Defense and CDK4/6 in Mesothelioma. Molecules, 28(11), 4380.
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3

FGFR-1c Activation Quantification in BaF3 Cells

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BaF3 murine lymphoid cells were transfected to express the single FGFR-1c isoform so that specific FGFR-1c activation can be quantified.38 (link) Cells were maintained in Roswell Park Memorial Institute 1640 media (Sigma Chemical, St. Louis, MO) supplemented with 10% newborn bovine serum (Sigma Chemical), 50-μM β-mercaptoethanol, 0.5 ng/mL murine recombinant interleukin-3 (PeproTech Inc, Rocky Hill, NJ), 2-mM L-glutamine, penicillin-streptomycin (“BaF3 culture medium”), and G418 antibiotic (600 μg/mL). FGFR-1c expressing BaF3 cells were washed twice in BaF3 “assay media” (“culture media” lacking both murine recombinant interleukin-3 and β-mercaptoethanol) and plated at a density of 30,000 cells per well in a 96-well assay plate in assay media containing heparin sulfate (1 μg/mL) and concentrations of recombinant WT FGF-1 and Cys-free mutants ranging from 0.02 to 5 nM (3.18 × 102–7.95 × 105 pg/mL). The cells were incubated for 36 h, and DNA synthetic activity was determined by adding 1 μCi of 3H-thymidine in 50 μL of BaF3 assay medium to each well. Cells were harvested after 4 h by filtration through glass fiber paper. Incorporated 3H-thymidine was counted on a MicroBeta plate scintillation counter (PerkinElmer, Waltham, MA).
Xia X., Kumru O.S., Blaber S.I., Middaugh C.R., Li L., Ornitz D.M., Sutherland M.A., Tenorio C.A, & Blaber M. (2016). Engineering a Cysteine-Free Form of Human Fibroblast Growth Factor-1 for “Second Generation” Therapeutic Application. Journal of pharmaceutical sciences, 105(4), 1444-1453.
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4

Culturing K562 and HELF cells

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K562, human chronic myelogenous leukemia cells, were obtained from the Institute of Hematology at the Chinese Academy of Medical Sciences (Beijing, People’s Republic of China). HELF, human embryonic lung fibroblast cells, were obtained from the Shanghai Institute of Cells at the Chinese Academy of Sciences (Shanghai, People’s Republic of China). They were maintained in RPMI 1640 medium supplemented with 10% heat-inactivated newborn bovine serum (Sigma-Aldrich), 100 U/mL penicillin, and 100 mg/mL streptomycin at 37°C in a humidified atmosphere with 5% CO2 and passaged once every 2 to 3 days.
Xu P., Wang R., Ouyang J, & Chen B. (2015). A new strategy for TiO2 whiskers mediated multi-mode cancer treatment. Nanoscale Research Letters, 10, 94.
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