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PiaA 36 is a laboratory equipment product manufactured by Cell Signaling Technology. It is a device used for the analysis and detection of protein interactions and signaling pathways. The core function of PiaA 36 is to facilitate the study of cellular communication and regulatory mechanisms.

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Lab products found in correlation

Actin, by Santa Cruz Biotechnology (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Rictor, by Cell Signaling Technology (2 mentions) Raptor, by Cell Signaling Technology (2 mentions) Gapdh, by Thermo Fisher Scientific (2 mentions) Gsk 3, by Merck Group (2 mentions) Phospho rps6kb1, by Cell Signaling Technology (2 mentions) Phospho pkc, by Cell Signaling Technology (2 mentions) Phospho akt, by Cell Signaling Technology (2 mentions) Pharosfx plus, by Bio-Rad (2 mentions)

2 protocols using piaa 36

1

Western Blot Analysis of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were separated using SDS-PAGE and transferred onto PVDF membranes. The antibodies were GFP 31 (link), 60 (link), FLAG (Sigma, F7425 and F3165), PiaA 36 (link), Tor (Cell Signaling, 2983), Rictor (Cell Signaling, 2114), Raptor (Cell Signaling, 2280), GAPDH (Thermo Fisher, MA5–15738), actin (Santa Cruz, Sc-1615), GSK-3 (Millipore, 05–412), phospho-RPS6KB1 (Cell Signaling, 9205), phospho-PKC (Cell Signaling, 2060), AKT (Cell Signaling, 9272) and phospho-AKT (serine 473) (Cell Signaling, 9271). Polyclonal rabbit antibodies to PKBA and PKBR1 were raised against the peptides, KNSDRKKRVNG and KKGNKNDETTP, respectively. Polyclonal rabbit antibodies to RacE and phospho-RacE (serine 192) were raised against the peptide REQQHPDPNSGKF and the phosphopeptide GMDKK(pS)QDGSS. Immunocomplexes were visualized using fluorescent-labeled secondary antibodies and detected using a Bio-Rad PharosFX Plus molecular imager. Images were analyzed using NIH ImageJ. The detailed information about the antibodies used in this study is provided in Supplementary Table 4.
Senoo H., Kamimura Y., Kimura R., Nakajima A., Sawai S., Sesaki H, & Iijima M. (2019). Phosphorylated Rho-GDP Directly Activates mTORC2 Kinase Toward AKT Through Dimerization with Ras-GTP to Regulate Cell Migration. Nature cell biology, 21(7), 867-878.
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2

Western Blot Analysis of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were separated using SDS-PAGE and transferred onto PVDF membranes. The antibodies were GFP 31 (link), 60 (link), FLAG (Sigma, F7425 and F3165), PiaA 36 (link), Tor (Cell Signaling, 2983), Rictor (Cell Signaling, 2114), Raptor (Cell Signaling, 2280), GAPDH (Thermo Fisher, MA5–15738), actin (Santa Cruz, Sc-1615), GSK-3 (Millipore, 05–412), phospho-RPS6KB1 (Cell Signaling, 9205), phospho-PKC (Cell Signaling, 2060), AKT (Cell Signaling, 9272) and phospho-AKT (serine 473) (Cell Signaling, 9271). Polyclonal rabbit antibodies to PKBA and PKBR1 were raised against the peptides, KNSDRKKRVNG and KKGNKNDETTP, respectively. Polyclonal rabbit antibodies to RacE and phospho-RacE (serine 192) were raised against the peptide REQQHPDPNSGKF and the phosphopeptide GMDKK(pS)QDGSS. Immunocomplexes were visualized using fluorescent-labeled secondary antibodies and detected using a Bio-Rad PharosFX Plus molecular imager. Images were analyzed using NIH ImageJ. The detailed information about the antibodies used in this study is provided in Supplementary Table 4.
Senoo H., Kamimura Y., Kimura R., Nakajima A., Sawai S., Sesaki H, & Iijima M. (2019). Phosphorylated Rho-GDP Directly Activates mTORC2 Kinase Toward AKT Through Dimerization with Ras-GTP to Regulate Cell Migration. Nature cell biology, 21(7), 867-878.
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