Cobas ampliprep taqman 48

Manufactured by Roche

Sourced in United States

The COBAS Ampliprep/TaqMan 48 is an automated sample preparation and real-time PCR system designed for in vitro diagnostic applications. It facilitates the extraction, amplification, and detection of nucleic acid targets from a variety of sample types.

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Lab products found in correlation

Epics xl flow cytometer, by Beckman Coulter (2 mentions) Au5800 chemistry analyzer, by Beckman Coulter (2 mentions) Facscount system, by BD (1 mentions) Cobas e601, by Roche (1 mentions)

Close spelling variants of this product

COBAS Ampliprep/TaqMan 48 real-time RT-PCR Test COBAS®AmpliPrep/COBAS®TaqMan® 48 System Roche COBAS AmpliPrep/Cobas TaqMan 48 test kit version 2.0 COBAS Ampliprep system v2.0/Taqman-48 system COBAS Ampliprep V2.0/Taqman-48 system COBAS Ampliprep/COBAS TaqMan 48 Analyser COBAS Ampliprep/Taqman 48 Version 2.0 COBAS Ampliprep/COBAS TaqMan 48 Analyzer COBAS AmpliPrep/TaqMan COBAS AmpliPrep/COBAS TaqMan

6 protocols using cobas ampliprep taqman 48

Monitoring HIV-1 Patients in Baoding

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Baoding People's Hospital is the designated hospital of ART and takes charge of anti-HIV therapy of all HIV-1-infected individuals in Baoding city. In this study, a total of 131 HIV-1-infected individuals were recruited in Baoding City before starting ART from January 2020 to December 2021. Their blood samples were collected in Baoding People's Hospital, and written informed consent was obtained from all subjects before blood collection. The baseline demographic characteristics were investigated using face-to-face interviews when we collected subjects’ blood samples. CD4⁺ T cell counts were determined using the BD FACSCount system (Becton Dickenson, CA, United States). Plasma HIV RNA levels were quantitatively tested using the Ampliform HIV-1 Monitor Test, version 1.5 (Roche, Cobas AmpliPrep/TaqMan 48, Switzerland). The detection limit threshold was <20 copies/ml.

Fan W., Wang X., Zhang Y., Meng J., Su M., Yang X., Shi H., Shi P, & Lu X. (2022). Prevalence of resistance mutations associated with integrase inhibitors in therapy-naive HIV-positive patients in Baoding, Hebei province, China. Frontiers in Genetics, 13, 975397.

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Comprehensive Bone Turnover and HIV Evaluation

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All laboratory testing was performed in the Clinical Laboratory Department of PUMCH. BTMs tested included the bone resorption marker, C-terminal cross-linking telopeptide of type-1 collagen (CTX), and bone formation marker, procollagen type 1 N-terminal propeptide (P1NP). 25-hydroxy vitamin D (25OHD), intact PTH (iPTH) and phosphorus were assayed as additional indicators of bone homeostasis. CTX, P1NP, 25OHD, and iPTH were assayed by an electrochemiluminescence immunoassay (MODULAR ANALYTICS E170, cobase 601, Roche Diagnostics, Mannheim, Germany). Phosphorus was assessed using a molybdate-based method (Beckman Coulter AU5800 Chemistry Analyzer, Beckman Coulter Inc., Brea CA, USA).
Plasma HIV-1 RNA viral load was measured by the COBAS Ampliprep/TaqMan 48 according to the manufacturer’s instructions (Roche Molecular Systems, Pleasanton, CA, USA; reference range 40–1,000,000 copies/mL). CD4⁺ T cell count was determined by 3-color flow cytometry (Epics XL flow cytometer, Beckman Coulter, USA). Freshly collected EDTA-anticoagulated whole blood was incubated with a panel of fluorescence-labeled monoclonal antibodies and isotype controls (FITC-CD4/PE-CD8/PE-Cy5-CD3, FITC-IgG1/PE-IgG1/PE-Cy5-IgG1). Cell number was acquired by flow cytometer after red blood cell lysis.

Hsieh E., Fraenkel L., Xia W., Hu Y.Y., Han Y., Insogna K., Yin M.T., Xie J., Zhu T, & Li T. (2014). Increased Bone Resorption During Tenofovir Plus Lopinavir/Ritonavir Therapy in Chinese Individuals with HIV. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 26(3), 1035-1044.

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Biomarker Profiling in HIV Patients

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Plasma DBP was measured using the Quantikine Human DBP enzyme-linked immunosorbent assay kit (R&D Systems, Inc., Minneapolis, MN). The bone resorption marker, C-terminal cross-linking telopeptide of type-1 collagen (CTX), and bone formation marker, total procollagen type 1 N-terminal propeptide (P1NP), 25OHD, and iPTH were assayed by an electrochemiluminescence immunoassay (cobas e 601, Roche Diagnostics, Mannheim, Germany). Phosphorus was assessed using a molybdate-based method (Beckman Coulter AU5800 Chemistry Analyzer, Beckman Coulter Inc., Brea, CA, USA). Plasma HIV-1 RNA viral load was measured by the COBAS Ampliprep/TaqMan 48 (Roche Molecular Systems, Pleasanton, CA, USA). CD4⁺ T cell count was determined by 3-color flow cytometry (Epics XL flow cytometer, Beckman Coulter, USA).

HSIEH E., FRAENKEL L., HAN Y., XIA W., INSOGNA K.L., YIN M.T., ZHU T., CHENG X, & LI T. (2016). Longitudinal Increase in Vitamin D Binding Protein Levels after Initiation of Tenofovir/Lamivudine/Efavirenz among Individuals with HIV. AIDS (London, England), 30(12), 1935-1942.

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Quantifying HIV-1 Viral Load in Plasma

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The HIV-1 viral load in plasma samples from infected individuals was determined with a COBAS Ampliprep/TaqMan48 real-time reverse transcriptase polymerase chain reaction (RT-PCR) Test (Roche Diagnostics, Indianapolis, Indiana, USA) according to the manufacturer's instructions. The lower detection limit of the assay was 50 HIV-1 RNA copies/ml.

Liu Y., Li X., Han Y., Qiu Z., Song X., Li B., Zhang H., Wang H., Feng K., Liu L., Wang J., Sun M, & Li T. (2020). High APRIL Levels Are Associated With Slow Disease Progression and Low Immune Activation in Chronic HIV-1-Infected Patients. Frontiers in Medicine, 7, 299.

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Determining HIV, TB Status from Samples

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Blood samples were collected to establish CD4 counts (PIMA platform; Alere, Waltham, MA), and HIV viral load (COBAS Ampliprep/Taqman 48; Roche, Pleasanton, CA). Induced sputum samples were collected using nebulized hypertonic saline. Samples were examined by Ziehl-Neelson stain microscopy and a single mycobacterial culture was performed on Lowenstein-Jensen media.

Bhadriraju S., Fadrosh D.W., Shenoy M.K., Lin D.L., Lynch K.V., McCauley K., Ferrand R.A., Majonga E.D., McHugh G., Huang L., Lynch S.V, & Metcalfe J.Z. (2020). Distinct lung microbiota associate with HIV-associated chronic lung disease in children. Scientific Reports, 10, 16186.

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Quantifying Viral Loads and Liver Fibrosis

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Plasma HIV RNA, HBV DNA and HCV RNA levels were quantified by the COBAS AmpliPrep/TaqMan48 real-time PCR system (Roche Molecular Systems, Pleasanton, CA, USA) in the Central Laboratory at PUMCH. Plasma samples were separated from whole blood by centrifugation within 4 h of collection and stored at −80°C until tested. The linear range of HIV RNA, HBV DNA and HCV RNA were 40–1,000,000 copies/mL (1.60–6.00 log₁₀ copies/mL), 20–170,000,000 IU/mL (1.30–8.23 log₁₀ IU/mL) and 15–100,000,000 IU/mL (1.18–8.0 log₁₀ IU/mL), respectively.
Two non-invasive markers, AST-to-platelet ratio index (APRI) [19 ] and the fibrosis-4 score (FIB4) [20 ], were used to evaluate the liver fibrosis of study participants. APRI was calculated according to Wai et al. [19 ]: (actual AST value divided by its upper normal limit considered as 40 U/L)/platelet counts (10⁹/L)×100. FIB4 score was calculated according to Sterling et al. [20 ]: [age (years)×AST (U/L)]/[platelets (10⁹/L)×(ALT U/L)^1/2].

Xie J., Han Y., Qiu Z., Li Y., Li Y., Song X., Wang H., Thio C.L, & Li T. (2016). Prevalence of hepatitis B and C viruses in HIV-positive patients in China: a cross-sectional study. Journal of the International AIDS Society, 19(1), 20659.

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