Metformin, 3,3-diaminobenzidine tetrahydrochloride (DAB), and anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304) and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Alexa Fluor 594-conjugated goat polyclonal anti-mouse IgG was purchased from Invitrogen (Sollentuna, Sweden). The avidin-biotinylated-peroxidase complex detection system (ABC kit) and DAB-Ni were purchased from Vector Laboratories Inc. (Burlingame, CA). The primary antibodies used for Western blot analysis and immunostaining in the present study, along with their species, molecular weight, dilutions, and sources are listed in Supplemental Table 1 .
Anti rabbit igg hrp conjugated goat a0545
Manufactured by Merck Group
Anti-rabbit IgG HRP-conjugated goat (A0545) is a laboratory reagent used for the detection of rabbit immunoglobulin G (IgG) in various immunoassays. The product consists of goat-derived antibodies that are conjugated to horseradish peroxidase (HRP), an enzyme that can be used to generate a detectable signal. This reagent is intended for research use only and its specific applications should be evaluated by the user.
Automatically generated - may contain errors
Lab products found in correlation
Avidin biotinylated peroxidase complex detection system abc kit, by Vector Laboratories (5 mentions)
Anti mouse igg horseradish peroxidase hrp conjugated goat a2304, by Merck Group (4 mentions)
Alexa fluor 594 conjugated goat polyclonal anti mouse igg, by Thermo Fisher Scientific (4 mentions)
Human chorionic gonadotropin (hcg), by Organon (3 mentions)
3 3 diaminobenzidine tetrahydrochloride dab, by Merck Group (2 mentions)
Alexa fluor 488 conjugated goat polyclonal anti rabbit igg, by Thermo Fisher Scientific (2 mentions)
Dab ni, by Vector Laboratories (1 mentions)
Metformin, by Merck Group (1 mentions)
Humulin nph, by Eli Lilly (1 mentions)
Diaminobenzidine dab, by Merck Group (1 mentions)
Ar sc 816, by Santa Cruz Biotechnology (1 mentions)
Flutamide, by Merck Group (1 mentions)
P21cip1, by Santa Cruz Biotechnology (1 mentions)
5 protocols using anti rabbit igg hrp conjugated goat a0545
1
Immunostaining and Western Blot Protocol
2
Insulin and hCG Signaling Pathway Analysis
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Human recombinant insulin (Humulin NPH) was from Eli Lilly Pharmaceuticals (Giza, Egypt), and hCG was from NV Organon (Oss, Holland). Metformin and 3,3-diaminobenzidine tetrahydrochloride (DAB), anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304), and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were from Sigma-Aldrich (St. Louis, MO). The primary antibodies used for Western blot and immunohistochemical analyses in the present study, their dilution, and sources are listed in Table S1. Alexa Fluor 594-conjugated goat polyclonal anti-mouse IgG was from Invitrogen (Sollentuna, Sweden). The avidin-biotinylated-peroxidase complex detection system (ABC kit) was from Vector Laboratories Inc. (Burlingame, CA). A detailed list of primers is provided in Table S2.
3
Immunohistochemical Analysis of Hormone Receptors
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Human chorionic gonadotropin (hCG) was from NV Organon (Oss, Holland). 17β-estradiol (E2), progesterone (P4), and 3,3-Diaminobenzidine tetrahydrochloride (DAB) were from Sigma-Aldrich (St. Louis, MO). The Avidin-biotinylated-peroxidase complex detection system (ABC kit) was from Vector Laboratories Inc. (Burlingame, CA). All antibodies were from the following sources: GLUT4 (ab33780) antibody was from Abcam (Cambridge, UK), cytokeratin 8/18 (#4546) antibody was from Cell Signaling Technology (Danvers, MA); estrogen receptor alpha (ERα, #6F11) and progesterone receptor A/B (PRA and PRB, #16) antibodies were from Novocastra Laboratories (Newcastle, UK); and estrogen receptor beta (ERβ, PPG5/10) antibody was from AbD Serotec (Oxford, UK). β-actin (P-0130), anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304), and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were from Sigma-Aldrich.
4
Immunohistochemical and Western Blot Analyses
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hCG was from NV Organon (Oss, Holland). Human recombinant insulin (Humulin NPH) was from Eli Lilly (Lilly Pharmaceuticals, Giza, Egypt). 3,3-diaminobenzidine tetrahydrochloride (DAB) was from Sigma-Aldrich (St. Louis, MO). The avidin-biotinylated-peroxidase complex detection system (ABC kit) was from Vector Laboratories Inc. (Burlingame, CA). The primary antibodies used for Western blot and immunohistochemical analyses in the present study, their dilution, and sources are listed in Supplemental Table 1 . Anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304), and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were from Sigma-Aldrich. Alexa Fluor 594-conjugated goat polyclonal anti-mouse IgG and Alexa Fluor 488-conjugated goat polyclonal anti-rabbit IgG were from Invitrogen (UK).
5
Molecular Mechanisms of Androgen Receptor Regulation
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DHT (a specific AR agonist, D-5027), flutamide (a specific AR antagonist, F-0397), and diaminobenzidine (DAB) were from Sigma-Aldrich (St. Louis, MO). Avidin-biotinylated-peroxidase complex detection system (ABC kit) was from Vector Laboratories Inc. (Burlingame, CA). All antibodies were from the following sources: AR (#5153, this antibody reacts with human tissues), AMPKα (#2532), phospho-AMPKα (phosphorylated threonine 172, #2535), and Ki-67 (#11882) antibodies were from Cell Signaling Technology (Danvers, MA); AR (sc-816, this antibody reacts with rodent tissues) and p21Cip1 antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA); and cysteine-rich protein 61 (CYR61, HPA029853) antibody was from Sigma-Aldrich. Anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304) and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were from Sigma-Aldrich. Alexa Fluor 594-conjugated goat polyclonal anti-mouse IgG, and Alexa Fluor 488-conjugated goat polyclonal anti-rabbit IgG were from Invitrogen (Life Technologies Europe BV, Stockholm, Sweden).
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