Apcmin

C57BL/6J and Apc^Min/+ mice were originally purchased from Jackson Laboratory and bred inhouse for the experiments. Mice were maintained in IVC cages and kept in a 12-h/12-h dark-light cycle at 20–22 °C and 40–60% humidity with free access to food and water. All the mice were fed with PicoLab®Rodent Diet 20-5053 (LabDiet, USA).

Housing of mice and in vivo experiments were performed in compliance with the European Communities Council Directive of 24, November 1986 (86/609/EEC) and national and institutional guidelines. Animal care and experimental procedures were approved by the Animal Care Committee of the Institute of Molecular Genetics (ref. 58/2017). Apc^cKO/cKO mice [41 (link)] were obtained from the Mouse Repository (National Cancer Institute, Frederick, MD, USA); Villin-CreERT2 mice [42 (link)] were kindly provided by Sylvie Robine (Institut Curie, Centre de Recherche, Paris, France); Apc^+/Min (C57BL/6J-ApcMin/J) [43 (link)], Ki67-RFP (Mki67tm1.1Cle/J) [44 (link)], Lgr5-EGFP-IRES-CreERT2 (B6.129P2-Lgr5tm1(cre/ERT2)Cle/J) [45 (link)], and Rosa26-tdTomato (B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J) [46 (link)] mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). The animals were kept under specific pathogen-free conditions. To induce Cre-mediated gene recombination, mice were gavaged with 1 mg tamoxifen (Merck). tamoxifen was dissolved in ethanol (100 mg/mL) and combined (1:9) with mineral oil (Merck) before administration. Mice were sacrificed by cervical dislocation at various time points after administration of a single dose (100 µL) of the tamoxifen/oil solution.

Animal experiments were approved by the National Animal Experiment Board of Finland. Mice were housed and monitored according to the Federation of European Laboratory Animal Science Associations guidelines and recommendations. The mice were weighed and their health was closely monitored during the experiment. The mouse lines Lef1^fl/fl (25 (link)), Apc^fl/fl (76 (link)), Lgr5-EGFP-IRES-Cre^ERT2 (4 (link)), Rosa26^LSL-TdTomato (Jackson Laboratory, stock no. 021875), Apc^Min/+ (Jackson Laboratory, stock no. 002020), Villin-Cre^ERT2 (Jackson Laboratory, stock no. 020282), and Villin-Cre (Jackson Laboratory, stock no. 021504) have been described previously. Lef1^fl/fl mice with mixed C57BL/6 and 129SV background were used after backcrossing to the C57BL/6 strain for >6 generations. All experiments were performed three times with independent cohorts. Approximately equal numbers of male and female mice of same age were used for all the experiments.

C57BL/6J (WT) and APC^min/+ mice were purchased from The Jackson Laboratory. Nlrp12^–/– mice were generated by Millennium Pharmaceuticals. Nlrp12^fl/fl mice were generated by our laboratory with the help of the UT Southwestern Mouse Transgenic Core facility. The background of all mice used in this study is C57BL/6J. Unless otherwise stated, mice of different genetic backgrounds were housed in separate cages, maintained in the same animal room, and used for in vivo and in vitro experiments. All mice were bred and maintained in a specific pathogen–free (SPF) facility at the UT Southwestern Medical Center. For cohousing experiments, 4-week-old WT mice were cohoused with Nlrp12^–/– mice for 30 days. All animal studies were conducted with age- and sex-matched mice.