Ary009
Manufactured by R&D Systems
Sourced in United States
The ARY009 is an analytical instrument designed for the detection and quantification of specific proteins or analytes in biological samples. It utilizes a technology that allows for the simultaneous measurement of multiple targets within a single sample. The core function of the ARY009 is to provide researchers with a robust and reliable tool for various applications, such as biomarker discovery, drug development, and diagnostic testing.
Automatically generated - may contain errors
Lab products found in correlation
Imagequant, by GE Healthcare (2 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (2 mentions)
Trex1, by Cell Signaling Technology (1 mentions)
Mouse xl cytokine array, by R&D Systems (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Nupage, by Thermo Fisher Scientific (1 mentions)
Human apoptosis array, by R&D Systems (1 mentions)
Ary003, by R&D Systems (1 mentions)
Ab692, by Abcam (1 mentions)
Ab25901, by Abcam (1 mentions)
Ab152130, by Abcam (1 mentions)
Ab106393, by Abcam (1 mentions)
Vybrant dio cell labeling solution, by Thermo Fisher Scientific (1 mentions)
Ab24533, by Abcam (1 mentions)
R cm 561, by Lonza (1 mentions)
Wst 8 assay, by Dojindo Laboratories (1 mentions)
Ary003b, by R&D Systems (1 mentions)
Proteome profiler, by R&D Systems (1 mentions)
11 protocols using ary009
1
Protein Immunoblotting and Apoptosis Assay
2
Western Blot Analysis of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell and tissue lysates were prepared in RIPA buffer (Pierce 89900) and quantified using a BCA assay (Pierce, #23227) kit. Equivalent amounts of protein were loaded on a 4–12% gradient SDS-polyacrylamide gel (NuPAGE, Life-Technologies) or a 8% SDS–polyacrylamide gel and transferred overnight onto polyvinylidene difluoride (PVDF) membranes. Membranes were blocked in 5% milk or 3% BSA and incubated with antibodies as indicated- Trex1 (Cell Signaling 12215, s 1:1,000 o/n), Fancf (Abnova, h00002188-bo1p 1:1,000 o/n), anti-β-actin antibody (Sigma, A5316, 1:10,000 1 h RT). Membranes were washed in TBST and incubated with secondary antibodies from Licor Biosciences were used goat anti mouse 925-68020 (1:15,000) and goat anti rabbit 925-32211 (1:15,000). Blots were scanned on the Licor Odyssey scanner according to manufacturer's instructions. For specific protein array experiments with membrane arrays -mouse XL cytokine Array (R&D Biosystems-ARY028) and human apoptosis array (R&D Biosystems ARY009), protocols were according to manufacturer's instructions.
3
Apoptosis Protein Expression Profiling
4
Apoptosis Protein Array Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were plated and treated as for Annexin V assays. Cell lysates from three experiments (100 μg protein each) were combined, incubated with human apoptosis protein or phosphoprotein arrays (ARY009 and ARY003; R&D Systems) and processed as per the manufacturer’s protocol. The films were scanned with transmission-mode scanner and pixel densities analyzed with ImageQuant (GE Healthcare, Chicago, IL, USA).
5
Apoptotic Protein Profiling in PC3 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptotic Protein arrays (R&D Systems, ARY009) were used to probe for alterations in protein amounts. PC3 cell lysates were prepared 24 h after transfection with PN1 expression vectors and 300 μg of lysate was diluted in blocking buffer. This solution was incubated with an apoptotic protein array containing 35 pro- or anti-apoptotic proteins overnight at 2–8°C. After 3× washing with PBS, reconstituted Detection Antibody Cocktail was added to the membrane and incubated for 1 hour. The membrane was then subjected to Streptavidin-HRP followed by a chemiluminescent reagent, per manufacturer's instructions.
6
Investigating IGF-1's Cytoprotective Role
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The capacity to IGF-1–engineered EDCs to withstand cell death was assessed after culture in hypoxic (1% oxygen), low-serum (1% serum) conditions for 48 hours by examining (1) proliferation using the WST-8 assay (Dojindo); (2) expression of early apoptotic markers using flow cytometry (559763; BD Biosciences); (3) expression of Bcl-2, Fos, and Jun prosurvival transcripts using qPCR (Integrated DNA Technologies); (4) expression of 35 apoptosis-related and stress-activated proteins using a membrane-based antibody array (ARY009; R&D Systems); and (5) expression of necroptosis markers (RIP1, ab106393; RIP3, ab152130; caspase-8, ab25901; FADD, ab24533; Abcam) using Western blot densitometry.
The prosurvival effect of IGF-1 overexpression on neighboring myocardium was explored during direct and indirect (Transwell; Corning) coculture with neonatal rat ventricular myocytes (NRVMs; R-CM-561; Lonza). EDCs were distinguished from NRVMs using Vybrant DiO Cell-Labeling Solution (Molecular Probes). NRVMs and EDC cocultures underwent analysis of (1) cell viability using the colorimetric WST-8 assay, (2) apoptosis using flow cytometry for annexin V, and (3) expression of the antiapoptotic protein Bcl-2 (ab692; Abcam) using immmunohistochemistry.
The prosurvival effect of IGF-1 overexpression on neighboring myocardium was explored during direct and indirect (Transwell; Corning) coculture with neonatal rat ventricular myocytes (NRVMs; R-CM-561; Lonza). EDCs were distinguished from NRVMs using Vybrant DiO Cell-Labeling Solution (Molecular Probes). NRVMs and EDC cocultures underwent analysis of (1) cell viability using the colorimetric WST-8 assay, (2) apoptosis using flow cytometry for annexin V, and (3) expression of the antiapoptotic protein Bcl-2 (ab692; Abcam) using immmunohistochemistry.
7
Evaluating p53 Targets in DPSC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In all, 200–400 µg protein lysates of shRNA-Control, shRNA-p53, MI-773-treated or vehicle-treated DPSC were used to evaluate p53 targets with human apoptosis array kit (ARY009, R&D systems; Minneapolis MN USA). Membranes were exposed to x-ray film for 2–10 min, the intensity of proteins was quantified by Image J software. The fold change of band density compared with controls was used to show the impact of treatment on protein expression.
8
Apoptosis Protein Expression Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The expression profile of apoptosis-related proteins was detected and analyzed using a human apoptosis array kit (ARY009), according to the manufacturer's instruction (R&D Systems, Minneapolis, MN). The membranes containing apoptosis-related antibodies were blocked with Array buffer for 1 h on a rocking platform and then incubated with lysates of untreated (DMSO) or Torin2 treated ATC cell lines (8505c, C643 and SW1736) overnight at 4°C. Chemiluminescent detection reagent was used after incubation with streptavidin-HRP conjugate. The membranes were scanned and pixel density was quantified by the mean of two replicate spot densities normalized to internal background using Image J software. Targets showing a two-fold difference in density to background ratio (increase or decrease) when compared to vehicle were selected.
9
Annexin V-Alexa Fluor 488 Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For early apoptosis analysis, Annexin V conjugated with Alexa Fluor 488 (A13201; Thermo Fisher) was used. 1 × 105 cells were seeded in six-well plates 1 day before treatment with IRRE or PDR3 at 500 nM. After staining with Annexin V-Alexa Fluor 488, confocal microscopy or flow cytometry was used to assess apoptosis. To discriminate dead cells, we used propidium iodide (PI) to stain cells. For determining apoptotic pathways, a human apoptosis array kit (ARY009; R&D Systems) was used following the manufacturer’s instruction. Image J was used to quantify the pixel intensity.
10
Apoptosis Protein Profiling after Irradiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell lysates obtained at 48 hours after irradiation (100 μg protein), were incubated with an array of antibodies against human apoptosis related proteins (ARY009; R&D Systems) and then were processed as per the manufacturer's protocol. The films were scanned with transmission-mode scanner and pixel densities analyzed with ImageQuant (GE Healthcare, Chicago, IL, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!