300 mhz nmr spectrometer

All absorbance measurements and spectral scans were carried out with the aid of a SPECORD 250 PLUS Analytikjena Spectrophotometer (Germany) with a 1 cm quartz cell. Field-emission scanning electron microscopy (FE-SEM; VEGA3 TESCAN, Czech Republic) and high-resolution transmission electron microscopy (HRTEM; JEOL JEM-2100, Japan) were used to study particle size, shape, and surface morphology. Fourier transform infrared spectra (FTIR) were recorded on a Nicolet 6700 ATR-FTIR spectrometer (Thermo Scientific, Germany). Particle size and zeta potential (ZP) of plain/uncoated SPIONs and the ADFS were determined by a Malvern Panalytical instrument (UK). A vibrating sample magnetometer (VSM, Lakeshore, Model 7410) was used to measure the magnetic properties of the synthesized nanomaterials. X-ray diffraction (XRD) spectra (Discover-D8, Bruker, USA) were used to unravel the crystal structure of the synthesized nanoparticles. ¹H NMR spectra were recorded with a Varian 300 MHz NMR spectrometer (Germany) with deuterated DMSO as a solvent. The pH measurements were carried out using a JENCO 6173 pH meter. The mass spectrum of the AD was recorded using a Q 1000 EX LC-MS Shimadzu (Japan).

Synthesis of CNI-Gly followed procedures previously developed for caging glutamate and GABA (Ellis-Davies, 2011 (link)). All chemicals were purchased from commercial sources and used as received unless otherwise noted. Reactions were monitored by thin-layer chromatography (TLC) on Merck KGaA glass silica gel plates (60 F254) and were visualized with UV light. NMR spectra were recorded on a Varian 300 MHz NMR spectrometer. The chemical shifts are reported in ppm using the solvent peak as the internal standard. Peaks are reported as: s = singlet, d = doublet, t = triplet, q = quartet, dd = doublet of doublets, m = multiplet. High resolution mass spectral data were obtained using an Agilent G1969A ToF LC-MS (Agilent, Santa Clara, CA, USA).
Reverse-phase chromatography used two systems. Analytical UPLC was carried out with a Waters Acuity Arc (Milford, MA, USA) using a BEH300 C-18 column (2 × 50 mm, 1.7 μm particle size) monitored with a 2998 PDA detector. Elution used a linear gradient elution (0-100% acetonitrile, in water with 0.1 % TFA for 6 min). Preparative HPLC was carried out using a Waters PrepLC using an Alltech Altima C-18 column (22 x 250 mm, 5.0 μm particle size) monitored with a 2489 detector at 254 nm. Isocratic elution was used (10 mL min^-1) with 25% MeCN in water with 0.1% TFA.

The concentration of BCG was measured by a SPECORD250PLUSAnalytikjena Spectrophotometer (Germany) by measuring the absorbance at 444 nm using a 1 cm quartz cell. A VEGA3 TESCAN field-emission scanning electron microscope (FESEM, Czech Republic) was used for studying particle size and surface morphology. The particle size and zeta potential (ZP) of naked SPIONs and ADFS materials were determined by DLS analysis using a Malvern Panalytical instrument (UK). Fourier transform infrared spectra (FTIR) were recorded using a Nicolet 6700 ATR-FTIR spectrometer (Thermo Scientific, Germany). The magnetic properties were investigated with a vibrating-sample magnetometer (VSM) (Lakeshore, model 7410). An X-ray diffraction (XRD) spectrometer (Discover-D8, Bruker, USA) was used to confirm the formation of the magnetite crystallographic structure. ¹H-NMR spectra were recorded using a Varian 300 MHz NMR Spectrometer (Germany) using DMSO as a solvent. TGA and DTA analyses have been carried out using a Shimadzu 50 instrument. The mass spectrum of SMS was recorded using an LC-MS instrument (Thermo Scientific).