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Estradiol cypionate

Manufactured by Merck Group
Sourced in United States

Estradiol cypionate is a synthetic estrogen used in laboratory settings. It is a form of the natural female sex hormone estradiol, which is chemically combined with the fatty acid cypionate to create a long-acting injectable formulation. Estradiol cypionate is primarily used for research and scientific applications.

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2 protocols using estradiol cypionate

1

PLGA-PEG Polymer-based Amphotericin B Delivery

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Poly(lactic-co-glycolic) acid (PLGA) (MW 15,000, ratio of lactide to glycolic acid molar ratio of 50:50), polyethylene glycol (PEG) (MW:3500), PLGA-PEG polymer material was customized from Jinan Daigang Biotechnology Co., LTD. Amphotericin B (AmB) powder (99.8% purity), crystal violet (CV),polyvinyl alcohol (PVA), dimethyl sulfoxide (DMSO), 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide (XTT), estradiol cypionate, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindo carbocyanine perchlorate (DiI) red fluorescence probe, 4,6-diamidino-2-phenylindole (DAPI), dialysis bags (molecular weight cut-off of 12 kDa) were purchased form Sigma-Aldrich (St. Louis, MO, USA). Tissue-Tek OCT Compound (Sakura Finetek U.S.A. Inc.). Sabouraud Dextrose Agar (SDA), Sabouraud Dextrose broth (SD) and Man Rogosa and Sharpe broth/Agar (MRS)were obtained from Huankai Microbial Co.(Guangdong, China). RPMI 1640 medium, Dulbecco’s Modifed Eagle’s Medium (DMEM), fetal bovine serum (FBS) and phosphate-buffered saline (PBS, pH = 7.0/5.5) was supplied by Thermo Fisher Scientific (Massachusetts, USA). ELISA kits for IL-2, IL-4, IL-6, IL-17, TNF-α and IFN-γ were purchased from Jingmei Biotechnology Co. (Jiangsu, China). CCK-8 (Dojindo, Japan).
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2

Estrogen Receptor Agonists in Animal Study

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Propylpyrazoletriol (PPT) and diarylpropionitrile (DPN) were purchased from Tocris Bioscience (Minneapolis, MN, USA). Zymosan, estradiol cypionate (EC) and β-estradiol-water soluble were purchased from Sigma Chemical Company (St. Louis, MO, USA). Veterinary pentabiotic was purchased from Fort Dodge - Zoetis (São Paulo, SP, Brazil). estradiol cypionate, PPT and DPN were dissolved in corn oil while Zymosan and β-estradiol-water soluble were suspended and dissolved in sterile saline (0.9%), respectively.
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