Dried samples of Rosa L. fruit were ground with a Retsch GM 200 electric grinder (Retsch GmbH, Hahn, Germany). The ground raw material was weighed on a Sartorius CP64-0CE analytical balance (Sartorius AG, Gottingen, Germany). Extracts of Rosa L. fruit samples were prepared in an ultrasonic bath Bandelin Sonorex Digital 10 P (Sigma-Aldrich, Darmstadt, Germany). Spectrophotometric studies were accomplished on a UV-visible light (UV-Vis) spectrophotometer M550 (Spectronic CamSpec, Garforth, UK). Qualitative and quantitative analysis of phenolics in extracts of rosehip fruit samples was accomplished using a Waters 2998 PDA detector (Waters, Milford, CT, USA).
Cp64 0ce
Manufactured by Sartorius
Sourced in Germany
The Sartorius CP64–0CE is a precision balance designed for laboratory use. It has a maximum weighing capacity of 64 g and a readability of 0.1 mg. The balance features a stainless steel weighing platform and a draft shield to ensure accurate measurements in a controlled environment.
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5 protocols using cp64 0ce
1
Phenolic Analysis of Rosa L. Fruit
2
Quantitative Analysis of Phenolic Compounds in Cherry Leaves
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For a quantitative analysis of the phenolic compounds, the cherry leaf samples were crushed to particles that could pass through a 355 μm sieve using an electrical mill “Retsch GM 200” (Retsch GmbH, Haan, Germany) and were stored in tightly closed vessels in a dark and dry place. The ground raw plant materials were weighed using electronic analytical scales “Sartorius CP64–0CE” (“Sartorius AG”, Göttingen, Germany). About a 2 g weight (accurate sample) of raw plant material was placed into a conical flask, into which was poured 15 mL of 40% ethanol. Extraction was performed in a dark place for 72 h. The obtained extract was filtered through a paper filter into a 25 mL volumetric flask. The paper filter was washed 2 times with 5 mL of 40% ethanol. The volume of the obtained extract was adjusted to 25 mL with 40% ethanol. Extracts were filtered through a membrane filter with a pore size of 0.22 μm (Carl Roth GmbH, Karlsruhe, Germany).
3
Extraction of Plum Fruit Phytochemicals
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The ground raw material and reagents were weighed by using electronic analytical scales “Sartorius CP64–0CE” (“Sartorius AG”, Göttingen, Germany). For the analysis, 2.5 g of lyophilized plum fruit powder was poured into a dark glass vial, subsequently adding 50 mL of 83.64% (v/v) ethanol with 0.1% hydrochloric acid. The extraction of plum fruit samples was carried out in a “Bandelin Sonorex Digital 10 P” (“Bandelin Electronic GmbH & Co. KG”, Darmstadt, Germany) ultrasonic bath. The extraction was carried out for 40 min at 80 Hz frequency and 678 W power. After the extraction, the samples were centrifuged using a “Heraeus Biofuge Stratos” (“Heraeus Holding GmbH”, Haan, Germany) centrifuge for 5 min at 9000 rpm at room temperature. The supernatant was then poured off the precipitations, filtered through cotton wool, and was poured into dark glass vials, which were stored in a fridge at 4 °C until the analysis. Prior to chromatography, the extracts were additionally filtered through membrane filters with 0.22 μm pore size.
4
Histopathological Evaluation of Cardiac Tissue
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For histopathological evaluation of cardiac tissue, mouse hearts were rinsed with PBS and fixed in PFA 4% (CP10; Roth) for at least 24 h. For analysis of the heart/body weight ratio, the pericardium, connective tissue, and vascular remains were excised carefully before hearts were weighed using a microbalance (CP64-0CE; Sartorius). Subsequently, hearts were dehydrated in a graded series of ethanol concentrations. Thereafter, tissues were embedded in paraffin (1.07158; Sigma-Aldrich). Murine sections were stained with hematoxylin (T865.1; Roth) and eosin (X883.1, day 21; Roth) to evaluate infiltration of hematopoietic cells or with Masson’s trichrome (010802, day 63; Bio-Optica) to assess cardiac fibrosis. Acute infiltration was evaluated semiquantitatively using an EAM score (0, no inflammatory infiltrates; 1, small foci of <100 inflammatory cells between myocytes; 2, larger foci of >100 inflammatory cells; 3, >10% of a cross section shows infiltration of inflammatory cells; 4, >30% of a cross section shows infiltration of inflammatory cells) as previously described (Valaperti et al., 2008 (link)). To assess fibrosis by morphometric evaluation, ∼30 fields per section were chosen randomly. Fibrosis was evaluated using a semiquantitative score (0, 0–1%; 1, 1–2%; 2, 2–3%; 3, 3–4%; 4, 4–5%; 5, >5% fibrosis). All analyses were performed in a blinded manner.
5
Histological Evaluation of Cardiac Tissue
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To evaluate the cardiac tissue histologically, mouse hearts were rinsed with PBS and fixated with PFA 4% (Roth, Karlsruhe, Germany). Analysis of the heart weight/body weight ratio was conducted using a microbalance (CP64-0CE, Sartorius, Göttingen, Germany) after carefully removing the pericardium, connective tissue, and vascular remains. Thereafter, hearts were dehydrated in a graded series of ethanol concentrations and subsequently embedded in paraffin (Sigma-Aldrich, St. Louis, MO, USA). To evaluate infiltration of leukocytes, sections were stained with hematoxylin (Roth) and eosin (Roth, H&E, day 21). The established EAM score (0: no inflammatory infiltrates; 1: small foci of <100 inflammatory cells between myocytes; 2: larger foci of >100 inflammatory cells; 3: >10% of a cross section shows infiltration of inflammatory cells; 4: >30% of a cross section shows infiltration of inflammatory cells) was used to evaluate leukocyte infiltration semi-quantitatively as previously described [2 (link)]. Analysis was performed in a blinded manner.
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