Jsm 6700f sem

The morphology and structures of sea urchin-like Au NPs were studied using a field emission scanning electron microscope (SEM, 3.0 kV, SU70, Hitachi, Japan), SEM studies were performed on a JEOL JSM-6700F SEM operating at 3.0 kV. Transmission electron microscopy (TEM) was performed by a Hitachi HT7700 operated under 100 kV. High-resolution TEM and (HRTEM) were taken by a JEOL JEM-2100F operated under 200 kV. The surface-enhanced Raman spectroscopy was performed using a Raman spectrometer (Horiba HR Evolution 800) with laser excitation at 633 nm.

Inverted phase contrast microscope, fluorescent inverted microscope and SEM were used to detect the morphological changes of A549 cells with or without drugs treatment. For the inverted phase contrast microscope analysis, the cells were treated with the method mentioned above. For fluorescent inverted microscope analysis, acridine orange that can bind to double DNA was added into the treated and untreated A549 cells (10⁴ cells/mL) inoculated in a 6-well plate, and then was set in darkness at room temperature for 0.5 h. Subsequently, the cells were washed 3 times with PBS buffer, and observed under fluorescent inverted microscope (Cairns, Germany). For SEM analysis, the drug treated and untreated cells were harvested and washed 3 times with 0.1M PBS buffer, then, fixed in glutaraldehyde solution at 4 °C for 3 h. After fixation, the samples were washed with PBS, and dehydrated using with different concentrations of ethanol, followed by displacing with isoamyl acetate for 30 min and drying in a dryness oven for 3–4 h. Finally, the cells were visualized under a JSM-6700F SEM (JEOL, Japan).

X-ray diffraction patterns (XRD) and Raman spectra of samples were recorded on X-ray diffractometer (D2 PHASER) and Labram HR evolution Raman spectrometer, respectively. The morphological characteristics of samples were surveyed by scanning electron microscope JEOL JSM-6700F SEM. The specific surface area of the samples was determined by measuring N₂ adsorption isotherm on the Tri Star 3000 instrument at 77 K. Magnetic saturation was determined using the MicroSense EZ9 vibrating sample magnetometer. The chemical bonds of samples were investigated by XPS (Escalab 250Xi, Thermo Scientific) and FTIR (Nicolet iS50 FTIR spectrometer), respectively. Electrochemical impedance spectra (EIS) were recorded using a three-electrode electrochemical workstation and the flat band of samples were determined using Autolab workstation PGS 302N. The working electrode was fabricated by dripping 100 μL of a mixed solution of UFBP, carbon black, and polyvinylidene difluoride (PVDF) with the ratio 80 : 10 : 10 in N-methyl pyrrolidinone solvent at a concentration of 10 mg mL⁻¹ on FTO substrate (with the surface area of 1 cm²) then dried at 40 °C for 48 hours. The electrolyte Na₂SO₄ 1 M was used for EIS measurements. The intermediates of CIP photodegradation were detected by LC-MSD-Trap-SL Agilent 1100.