B6.129s7 ifngtm1ts j

All in vivo procedures were performed in accordance with the University of Rochester’s University Committee on Animal Resources approved guidelines. Six- to eight-week-old wildtype and age-matched Ifng^−/− (B6.129S7-Ifng^tm1Ts/J) C57BL/6J mice were obtained from The Jackson Laboratory (Bar Harbor, ME, USA) and from a generous gift by Edith Lord, PhD. Animals were given at least one week to acclimate before establishment of subcutaneous tumors.

C57BL/6 (WT), CD45.1 (B6.SJL-Ptprc^aPepc^b/BoyJ), CD90.1 (B6.PL-Thy1^a/CyJ), OT-I (C57BL/6-Tg(TcrαTcrβ)1100Mjb/J), TCRα KO (B6.129S2-Tcrα^tm1Mom/J), IFNγ KO (B6.129S7-Ifng^tm1Ts/J) and TAP KO (B6.129S2-Tap1^tm1Arp/J) mice were purchased from Jackson Laboratories (Bar Harbor, ME). Vα2var mice [24 (link)] were bred at Jackson Laboratories (Bar Harbor, ME). Mice were 6 to 10 weeks old at the start of all experiments. Mice infected with M. tuberculosis were housed in a biosafety level 3 facility under specific pathogen-free conditions at DFCI or at UMMS.

All mice procedures reported in this study were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Illinois at Urbana-Champaign under protocol numbers 17188 and 20188. Breeder pairs of interferon gamma knock out (IFN-γ KO) mice (B6.129S7-Ifng^tm1Ts/J) were purchased from the Jackson laboratory for mating, and an in-house mouse colony was maintained. Four- to 6- week old IFN-γ KO mice (n = 5 mice per cage) were used for generating and passaging stable transgenic C. parvum strain. Both male and female IFN-γ KO mice (age- and sex-matched) were used for infection experiments. Mice infected with C. parvum were monitored for weight loss, fur ruffling, hunched posture and inactivity, and any animal showing a weight loss of ≥= 15% was euthanized.

B6.CD45.1, B6.CD45.2, (B6.CD45.1xCD45.2)F1, B6.EYFP.Rag1^-/- and B6.Rag2^-/-.CD45.1Cg were used in this study, bred and maintained under specific-pathogen free (SPF) conditions at the Biological Services Facility, University of York. BM cells from mice lacking the Ifngr2 gene (IFNγ-R2 KO) on a B6 background were generously provided by Dr. Grainger (University of Manchester, UK) [61 (link)]. BM cells from TNF receptor double KO mice (Tnfrsf1-dKO B6.129S mice) [62 ] backcrossed >10 generations to C57BL/6 mice were provided by Dr. Lindbom (Lund University, Sweden). IFNγ-KO (B6.129S7-Ifngtm1Ts/J, stock no. 002287) mice were obtained from the Jackson Laboratory. All mice were between 5–8 weeks of age at the start of experimental work. Mice were infected via the lateral tail vein with 3x10⁷ amastigotes of the Ethiopian strain of L. donovani (LV9) or tandem Tomato fluorescent protein expressing L. donovani (tdTom-L. donovani). Spleen parasite burden was expressed as Leishman-Donovan units (LDU), where LDU was equal to the number of parasites/1000 host nuclei multiplied by the organ weight in milligrams. BM parasite burden was determined as the number of parasites/1000 host nuclei.