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Dp2 bsw camera system

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The DP2-BSW camera system is a digital microscope camera designed for scientific and laboratory applications. It features a high-resolution sensor and advanced imaging capabilities to capture detailed micrographs. The camera system is compatible with a range of microscopes and can be used for various imaging tasks in research and analysis settings.

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Lab products found in correlation

Permount, by Thermo Fisher Scientific (2 mentions) Bx51 microscope, by Olympus (2 mentions) Xylene, by Thermo Fisher Scientific (2 mentions) X51 microscope, by Olympus (2 mentions) Dibutylphthalate polystyrene xylene (dpx), by Merck Group (2 mentions) Abc elite kit, by Vector Laboratories (2 mentions) 3 3 diaminobenzidine (dab), by Merck Group (1 mentions)

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DP2-BSW (69 citations) DP2 camera (5 citations)

4 protocols using dp2 bsw camera system

1

Immunohistochemical Localization of M1R, VGluT1, and VGluT2 in Amygdala

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Prior to the electron microscopic studies amygdalas from three brains were used for light microscopic immunohistochemistry, including trials to determine appropriate antibody dilutions. The same immunohistochemical procedures used for the electron microscopic studies were employed, except the sections were not osmicated and embedded for electron microscopy. Some sections were processed for light microscopic single-labeling of M1R, VGluT1, and VGluT2 using the same chromogens used in the electron microscopic study, whereas others were processed for light microscopic M1R/VGluT1 and M1R/VGluT2 double-labeling using the same chromogens used in the electron microscopic study. Following immunohistochemical processing, sections were mounted on gelatinized slides, dried overnight, dehydrated in ascending alcohols, cleared in xylene, and coverslipped with Permount (Fisher). Sections were analyzed using an Olympus BX51 microscope and digital light micrographs were taken with an Olympus DP2-BSW camera system.
McDonald A.J., Jones G.C, & Mott D.D. (2019). Diverse Glutamatergic Inputs Target Spines Expressing M1 Muscarinic Receptors in the Basolateral Amygdala: An Ultrastructural Analysis. Brain research, 1722, 146349.
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2

Immunohistochemical Detection of MOR in Rat Brain

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Light microscopic MOR localization was performed in rats using a rabbit antibody to MOR (catalog #24216; ImmunoStar, Hudson, WI). All antibodies were diluted in PBS containing 0.4% Triton X-100 and 1% normal goat serum. Sections were incubated in the MOR antibody (1:1000) overnight at 4°C and then processed using a rabbit ABC kit with DAB (diaminobenzidine 4HCl, Sigma Chemical Co., St. Louis, MO, USA) as a chromogen to generate a brown reaction product. After the reactions, sections were mounted on gelatinized slides, dried overnight, dehydrated in ethanol, cleared in xylene, and coverslipped in Permount (Fisher Scientific, Pittsburgh, PA, USA). Sections were analyzed using an Olympus BX51 microscope, and digital light micrographs were taken with an Olympus DP2-BSW camera system. Brightness and contrast were adjusted using Photoshop 6.0 software.
Zhang J., Muller J.F, & McDonald A.J. (2015). Mu Opioid Receptor Localization in the Basolateral Amygdala: An Ultrastructural Analysis. Neuroscience, 303, 352-363.
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3

Localization of Muscarinic Acetylcholine Receptor 1

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Localization of m1Rs was performed using the avidin-biotin immunoperoxidase (ABC) technique in brains M58 and M102. Sections through the amygdala were incubated in a rabbit anti-m1R antibody (1:800; catalog # M-9808; MilliporeSigma, Burlington, MA; RRID: AB_260731) for 48 h at 4° C and then processed for the avidin-biotin immunoperoxidase technique using a biotinylated goat anti-rabbit secondary antibody (1:400), and an Elite ABC kit (Vector Laboratories, Burlingame, CA). DAB (3, 3’-diaminobenzidine-4HCl, Sigma) was used as a chromogen to generate a brown reaction product. Antibodies were diluted in 1% normal goat serum, and 1% bovine serum albumin in 0.05 M PBS containing 0.4% Triton X-100. Following the immunohistochemical procedures, sections were mounted on gelatinized slides, dried overnight, dehydrated in descending ethanols, cleared in xylene (Fisher Scientific, Pittsburgh, PA), and coverslipped with DPX (MilliporeSigma). Sections were analyzed using an Olympus X51 microscope, and digital light micrographs were taken with an Olympus DP2-BSW camera system. Brightness and contrast were adjusted using Adobe Photoshop CS2 software.
McDonald A.J, & Mott D.D. (2021). Neuronal Localization of m1 Muscarinic Receptor Immunoreactivity in the Monkey Basolateral Amygdala. The Journal of comparative neurology, 529(10), 2450-2463.
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4

Immunohistochemical Localization of CB1 Receptors in Monkey Brain

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Localization of CB1Rs in the monkey BNC was performed using the avidin-biotin immunoperoxidase (ABC) technique. The primary antibody to CB1R (1:500; catalog # CB1-Rb-Af380; Frontier Institute Co., Shinkonishi, Ishikari City, Hokkaido, Japan; RRID AB_2571591) was raised in rabbit. The diluent for all antibodies in this study was a mixture of 3% normal goat serum, 1% bovine serum albumin, and 0.4% Triton X-100 in 0.05M phosphate buffered saline (PBS, pH 7.4). Coronal sections at regular intervals through the rostrocaudal extent of the amygdala were incubated in primary antibody for 24 h at 4° C and then processed for the avidin-biotin immunoperoxidase technique using a biotinylated goat anti-rabbit secondary antibody (1:400), and an Elite ABC kit (Vector Laboratories, Burlingame, CA). DAB (3, 3'-diaminobenzidine-4HCl, Sigma) was used as a chromogen to generate a brown reaction product. Following the immunohistochemical procedures, sections were mounted on gelatinized slides, dried overnight, dehydrated in descending ethanols, cleared in xylene (Fisher Scientific, Pittsburgh, PA), and coverslipped with DPX (Sigma). Sections were analyzed using an Olympus X51 microscope, and digital light micrographs were taken with an Olympus DP2-BSW camera system. Brightness and contrast were adjusted using Photoshop CS2 software.
, & McDonald A.J. (2020). Expression of the type 1 cannabinoid receptor (CB1R) in CCK-immunoreactive axon terminals in the basolateral amygdala of the rhesus monkey (Macaca mulatta). Neuroscience letters, 745, 135503.
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