Log In Sign up
The largest database of trusted experimental protocols

S 4700 field emission scanning electron microscope

Manufactured by Hitachi
Visit Supplier
Sourced in Japan, United States, United Kingdom

The S-4700 field emission scanning electron microscope by Hitachi is a high-resolution imaging device designed for advanced microscopy applications. It utilizes a field emission source to generate a focused electron beam, enabling high-resolution observation and analysis of a wide range of samples.

Automatically generated - may contain errors

Lab products found in correlation

Es 2030, by Hitachi (2 mentions) Isoamyl acetate, by Junsei (2 mentions) Osmium tetroxide, by Electron Microscopy Sciences (2 mentions) Paraformaldehyde glutaraldehyde buffer, by Junsei (2 mentions) Ac240ts, by Oxford Instruments (1 mentions) Ht7700 transmission electron microscope, by Hitachi (1 mentions) Glutaraldehyde, by Electron Microscopy Sciences (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Su 70 field emission scanning electron microscope, by Hitachi (1 mentions) Tecnai g2 f20, by Thermo Fisher Scientific (1 mentions) Tga dsc 3, by Mettler Toledo (1 mentions) Jem 2100, by JEOL (1 mentions) Dmlb light microscope, by Leica camera (1 mentions) Tecnai g2 20 x twin, by Thermo Fisher Scientific (1 mentions) Image pro plus 7, by Media Cybernetics (1 mentions) Emitech k550x sputter coater, by Quorum Technologies (1 mentions) 208hr high resolution sputter coater, by Cressington (1 mentions) Gallium 3 meso tetraphenylporphyrine chloride gatp, by Frontier Scientific (1 mentions) Unity inova 500 nb, by Agilent Technologies (1 mentions) D90 dslr camera, by Nikon (1 mentions)

46 protocols using s 4700 field emission scanning electron microscope

1

Comprehensive Characterization of MWCNTs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fourier Transform Infrared Spectroscopy (FTIR, Digilab FTS 7000) equipped with diamond Attenuated Total Reflection (ATR) crystal was used to investigate the chemical properties of pristine and purified MWCNTs. Scans ranging from 1000 to 4000 cm−1 were collected.
Morphology and elemental quantitative analyses of pristine and purified MWCNTs (1 mg/mL sample on silica wafer) were performed on a Hitachi S-4700 Field Emission Scanning Electron Microscope (Hitachi High-Technologies Corporation) containing a S-4700 detector combining secondary (SE) and backscattered (BSE) electron detection and operating at 20 KV. For Energy Dispersive X-Ray Spectroscopy (EDX) results are shown as weight percent of a given element relative to the most dominant element present in the sample.
The average length distribution of the pristine and purified MWCNTs was evaluated using tapping mode Atomic Force Microscopy (AFM) performed in air (Asylum Research, AC240TS, 50 to 90 kHz). At least 3 scans of 10 μm × 10 μm were acquired for each of the sample being analyzed and a minimum of 30 individual MWCNTs were measured to obtain an average length distribution.
Dong C., EIdawud R., Sargent L.M., Kashon M.L., Lowry D., Rojanasakul Y, & Dinu C.Z. (2014). Towards Elucidating the Effects of Purified MWCNTs on Human Lung Epithelial cells. Environmental science. Nano, 1(6), 95-603.
+ Open protocol
+ Expand
2

Structural Characterization of MIL-160 and ZIF-8

Check if the same lab product or an alternative is used in the 5 most similar protocols
Surface morphology and elemental composition of MIL-160 and ZIF-8 were investigated using Hitachi S-4700 Field Emission Scanning Electron Microscope (Hitachi High-Technologies Corporation) equipped with energy dispersive X-ray (EDX) spectroscopy. For these analyses, dry powders of the samples were mounted onto carbon tape and investigated using an accelerating voltage of 5.0 kV.
Crystalline phases of the synthesized MIL-160 and ZIF-8 MOFs were confirmed by powder x-ray diffraction (PXRD) analysis with experiments performed at room temperature under ambient conditions on PANalytical X’Pert Pro X-ray Diffractometer with CuKa radiation at 40 kV and 40 mA, respectively.
Wagner A., Liu Q., Rose O.L., Eden A., Vijay A., Rojanasakul Y, & Dinu C.Z. (2019). Toxicity screening of two prevalent metal organic frameworks for therapeutic use in human lung epithelial cells. International Journal of Nanomedicine, 14, 7583-7591.
+ Open protocol
+ Expand
3

Elemental Analysis of SWCNTs via EDX-S

Check if the same lab product or an alternative is used in the 5 most similar protocols
EDX-S was used to perform elemental analysis of SWCNT samples. Data were collected on a LEO 1530 VP scanning electron microscope equipped with an energy-dispersive X-ray analyzer (Hitachi S-4700 Field Emission Scanning Electron Microscope, Hitachi High Technologies Co., Tokyo, Japan). A few drops of SWCNT dispersion in cell culture medium were placed on a silicon wafer and allowed to air-dry. The silicon wafer was then mounted on an aluminum stub for EDX-S analysis.
Manke A., Luanpitpong S., Dong C., Wang L., He X., Battelli L., Derk R., Stueckle T.A., Porter D.W., Sager T., Gou H., Dinu C.Z., Wu N., Mercer R.R, & Rojanasakul Y. (2014). Effect of Fiber Length on Carbon Nanotube-Induced Fibrogenesis. International Journal of Molecular Sciences, 15(5), 7444-7461.
+ Open protocol
+ Expand
4

Visualization of Baculovirus Occlusion Bodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
SpexNPV-244.1 OBs were subjected to cryofixation in a Quorum PP2000 cryo-prep chamber (Quorum Technologies, East Sussex, UK) and visualized with an S-4700 field emission scanning electron microscope (Hitachi High Technologies America, Inc., Dallas, TX, USA) as previously described [18 (link)]. For transmission electron microscopy, OBs were subjected to chemical fixation and embedded in LX-112 resin as previously described [18 (link)]. Ultrathin sections of embedded OBs were cut and visualized with a Hitachi HT-7700 transmission electron microscope (Hitachi High Technologies America, Inc., Dallas, TX, USA).
Escasa S.R., Harrison R.L., Mowery J.D., Bauchan G.R, & Cory J.S. (2019). The complete genome sequence of an alphabaculovirus from Spodoptera exempta, an agricultural pest of major economic significance in Africa. PLoS ONE, 14(2), e0209937.
+ Open protocol
+ Expand
5

Ultrastructural Analysis of Caprin2 Mutant Lenses

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Scanning electron microscopy (SEM) was performed on 1 month old control (Pax6GFPCre:Caprin2+/cKO) and Caprin2 cKO/cKO mutant lenses as previously described (Scheiblin et al., 2014 (link)). Briefly, whole eyes were dissected without delay from control and mutant mice after euthanization and treated with a fixative containing 0.08M sodium cacodylate pH 7.4, 1.25% glutaraldehyde, 1% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 3 hours. The lens were dissected from the eye and transferred to fresh cold fixative for 48 hours. The lenses were washed and the lens capsule along with a few layers of fiber cells were removed from one hemisphere of the lens in order to view the fiber cell ultrastructure. The peeled lenses were then dehydrated through an alcohol dilution series and hexamethyldisilazane (HMDS, Sigma, St. Louis, MO) dilution series (diluted in ethanol). Lenses were then subjected to sputter coating with gold/palladium for 2.5 min before imaging with Hitachi S-4700 Field Emission Scanning Electron Microscope (Tokyo, Japan). The analysis was performed on eight biological replicates for both control and mutants.
Dash S., Dang C.A., Beebe D.C, & Lachke S.A. (2015). Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly. Developmental dynamics : an official publication of the American Association of Anatomists, 244(10), 1313-1327.
+ Open protocol
+ Expand
6

Freeze-Fracture Scanning Electron Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freeze-fracture scanning electron microscopy (FFSEM) was carried out using a Hitachi S-4700 field emission scanning electron microscope, as described in ref. 13 (link).
French D.J., Brown A.T., Schofield A.B., Fowler J., Taylor P, & Clegg P.S. (2016). The secret life of Pickering emulsions: particle exchange revealed using two colours of particle. Scientific Reports, 6, 31401.
+ Open protocol
+ Expand
7

Borrelia burgdorferi SEM Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

B. burgdorferi cultures were collected by centrifugation at 10,000 × g for 10 min at 4°C, the cell pellet resuspended in fixative (20 mM sodium cacodylate, pH 6 with 2.5% v/v glutaraldehyde) and cells were fixed overnight at 4°C. Cells were then centrifuged again, washed once in ddH2O and fixed in 2% osmium tetroxide for 2 h at 4°C. Cell pellets were washed twice in ddH2O, resuspended in ddH2O and the cells were loaded onto a 0.6 μm filter using a 1-ml syringe. Cells were gently dehydrated for 10 min each in a graded ethanol series; 35%, 50%, 70%, 90%, 95%, and twice in 100% EtOH using a 1-ml syringe. The filter was removed after the final 100% EtOH wash and placed in 100% hexamethyldisilazane for 30 min. The filter was air dried and placed on an adhesive carbon tab on a 13 mm aluminum stub. Filters with bacteria were coated with gold and palladium in a Pelco Model 3 sputter coater for 30 sec. After coating, samples were imaged in a Hitachi S-4700 Field Emission scanning electron microscope.
Drecktrah D., Lybecker M., Popitsch N., Rescheneder P., Hall L.S, & Samuels D.S. (2015). The Borrelia burgdorferi RelA/SpoT Homolog and Stringent Response Regulate Survival in the Tick Vector and Global Gene Expression during Starvation. PLoS Pathogens, 11(9), e1005160.
+ Open protocol
+ Expand
8

Dextran-coated Magnetite Nanoparticles Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
QT conjugated Fe3O4 nanoparticles (NPs) were prepared based on the existing literature with some modifications.[33 (link)] First, dextran-coated Fe3O4 NPs were synthesized through a chemical coprecipitation method. Briefly, a mixture including of FeCl3 anhydrous, FeCl2, and dextran dissolved in deionized (DI) water was put into a three-neck flask equipped with a mechanical stirrer. After the mixture was stirred and mixed completely, ammonia solution was dropped into the mixture with vigorous stirring under argon protection, until the pH of the solution reached 9. This solution was kept at 90°C for 2 h with constant stirring, and then the resultant precipitate was collected with the help of a strong external magnet. The supernatant was washed several times with DI water and ethanol and dried in oven at 70°C overnight.
QT conjugated magnetite NPs (QT-SPION) were prepared by addition of QT to dextran-coated Fe3O4 NPs. The FT-IR spectroscopy and X-ray diffraction (XRD) were recorded. The morphological features were obtained on a Hitachi S–4700 field emission-scanning electron microscope, equipped with an energy dispersive X-ray analysis detector.
Lered for various time intervals.
Najafabadi R.E., Kazemipour N., Esmaeili A., Beheshti S, & Nazifi S. (2018). Quercetin Prevents Body Weight Loss Due to the Using of Superparamagnetic Iron Oxide Nanoparticles in Rat. Advanced Biomedical Research, 7, 8.
+ Open protocol
+ Expand
9

Scanning electron microscopy of adult worms

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adult worms from the mice were isolated from the portal vein and mesenteric veins by portal perfusion method [52 (link)]. After washing with PBS, the worms were fixed in 2.5% glutaraldehyde for 60 min at 4°C. Worms were then rinsed twice with 5% sucrose. After that worms were incubated with 1% osmium tetroxide for 60 min, increasing concentrations of ethanol (50%, 70%, 80%, 90%, and 100%; 10 min each) were used to dehydrate the worms. Worms were critically point-dried and sputter-coated with gold. A HITACHI S-4700 field emission scanning electron microscope (Hitachi Ltd, Tokyo, Japan) was used to visualize the worms.
Lam H.Y., Liang T.R, & Peng S.Y. (2021). Ameliorative effects of Schisandrin B on Schistosoma mansoni-induced hepatic fibrosis in vivo. PLoS Neglected Tropical Diseases, 15(6), e0009554.
+ Open protocol
+ Expand
10

Ultrastructural Analysis of Bladder Biopsy Specimens

Check if the same lab product or an alternative is used in the 5 most similar protocols
The bladder biopsy specimens were prepared and fixed with glutaraldehyde and osmium tetroxide as described above. The specimens were then dehydrated through a graded series of ethanol till 100% ethanol, and the solution was replaced with 100% acetone. The specimens were critical-point dried and sputter coated with gold. They were then examined under a Hitachi S-4700 field emission scanning electron microscope at 15 kV. In both groups, the umbrella cell size and microplicae of the cell membrane were observed, and a 4-point scale was used for grading. All EM findings were graded by a single investigator who was blinded to the clinical results.
Lee Y.K., Jiang Y.H., Jhang J.F., Ho H.C, & Kuo H.C. (2022). Changes in the Ultrastructure of the Bladder Urothelium in Patients with Interstitial Cystitis after Intravesical Injections of Platelet-Rich Plasma. Biomedicines, 10(5), 1182.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!