Mab417

Manufactured by R&D Systems

Sourced in United States

MAB417 is a monoclonal antibody produced by R&D Systems. It is intended for use in various laboratory and research applications.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant murine il 33, by BioLegend (1 mentions) Mab17061, by R&D Systems (1 mentions) Facscalibur cytometer, by BD (1 mentions) L2880, by Merck Group (1 mentions) Triamcinolone acetonide, by Thermo Fisher Scientific (1 mentions) Sh 4 54, by Selleck Chemicals (1 mentions) Ruxolitinib, by Selleck Chemicals (1 mentions) Mab1835, by R&D Systems (1 mentions) Anti cd28 37, by BioLegend (1 mentions) Anti cd3 17a2, by BioLegend (1 mentions) Igg1 control, by BioLegend (1 mentions) L4391 1mg, by Merck Group (1 mentions) Alzet model 2002, by Durect (1 mentions)

9 protocols using mab417

Malaria Infection and Cerebral Malaria Model

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C57BL/6 red blood cells infected with Plasmodium berghei ANKA parasites expressing a green fluorescent protein (PbA GFPcon 259cl2, MRA-865, deposited by CJ Janse and AP Waters) were stored in liquid nitrogen and thawed and passed into wild-type mice that served as parasite donor. All mice, unless otherwise stated, were inoculated intravenously (i.v.) into the tail vein with 1×10⁴ parasitized red blood cells (pRBC). Parasitemia was monitored daily (from day 4) by flow cytometry using the FL3 channel (PbA-GFP) and TER-119 APC (erythrocytes) in a BD FACScalibur cytometer (BD Biosciences). Clinical score was assessed using the following clinical scale: 1 = no signs; 2 = ruffled fur and/or abnormal posture; 3 = lethargy; 4 = reduced responsiveness to stimulation and/or ataxia and/or respiratory distress/hyperventilation; and 5 = prostration and/or paralysis and/or convulsions. All animal that reachs stage 4 developped ECM. Recombinant murine IL-33 (Biolegend) was injected intraperitoneally (0.2 μg/mouse/200 μl) daily, routinely from the beginning of infection (day 0). As a control for IL-33 effects, non-infected (NI) mice also received IL-33 for 5 consecutive days. For some experiments, mice were administered with anti-Siglec F (MAB17061, R&D Systems, 50 μg daily) or anti-IL-10 (MAB417, R&D Systems, 40 μg daily) or appropriate isotype control Abs.

Besnard A.G., Guabiraba R., Niedbala W., Palomo J., Reverchon F., Shaw T.N., Couper K.N., Ryffel B, & Liew F.Y. (2015). IL-33-Mediated Protection against Experimental Cerebral Malaria Is Linked to Induction of Type 2 Innate Lymphoid Cells, M2 Macrophages and Regulatory T Cells. PLoS Pathogens, 11(2), e1004607.

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Spleen Cell Stimulation Assay

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Single-cell suspensions from spleen were adjusted to 4.0×10⁶ cells/mL in sterile RF10M and 2.0×10⁵ cells dispensed into wells of a sterile 96-well flat bottom plate (Corning #353072) The cells were mixed with an equal volume of RF10M lacking or containing the following reagents (source and final concentration shown in parentheses): LPS (Sigma, L2880, 10 μg/mL), IL10 blocking antibody (R&D Systems, MAB417, 10 ng/mL), Rat IgG1 control for IL10 blocking antibody (R&D Systems, MAB005, 10 ng/mL in PBS), recombinant mouse IL10 (Biolegend, 575802, 100 ng/mL), ruxolitinib (Selleckchem, S1378, 10 nM), SH-4–54 (Selleckchem, S7337, 10 nM), or triamcinolone acetonide (Alfa Aesar, J6354803, 10 nM). DMSO was used as a vehicle control for the small molecule inhibitors. Cells were cultured for 72h at 37 °C in a humidified incubator with 5% CO₂.

Palmer V.L., Worth A.N., Scott R.L., Perry G.A., Yan M., Li Q.Z, & Swanson P.C. (2018). IL10 restrains autoreactive B cells in transgenic mice expressing inactive RAG1. Cellular immunology, 331, 110-120.

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Targeting IL-10 and TGF-β in Colitis

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Mice in DSS + PC group were further intraperitoneally injected with 50 μg mouse anti-IL-10 and/or anti-TGF-β-1, -2, -3 antibodies (MAB417 and MAB1835, R&D Systems, Minnesota, USA) on day 0. This regimen was followed by intraperitoneal injections of 100 μg antibodies on day 2, and an additional 50 μg antibody on days 4 and 6. Control groups received equivalent amounts of rat-IgG or mouse-IgG (R&D Systems), following the same protocol50 (link).

Liu T., Ren J., Wang W., Wei X.W., Shen G.B., Liu Y.T., Luo M., Xu G.C., Shao B., Deng S.Y., He Z.Y., Liang X., Liu Y., Wen Y.Z., Xiang R., Yang L., Deng H.X, & Wei Y.Q. (2015). Treatment of dextran sodium sulfate-induced experimental colitis by adoptive transfer of peritoneal cells. Scientific Reports, 5, 16760.

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Microglia Modulation of NPC Proliferation

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NPC neurospheres were dissociated into single cells and plated onto PDL-coated multi-chamber glass slides (25,000 cells per chamber) (LabTek II) in SFM. One day following cell plating, the media was changed to 50% NPC SFM and 50% MCM. The following experimental groups were assessed: (1) MCM alone—50% NPC SFM + 50% fresh microglia media; (2) M0—50% NPC SFM + 50% M0 MCM; (3) M1—50% NPC SFM + 50% M1 MCM; (4) M2—50% SFM + 50% M2 MCM. The polarizing factors used to convert microglia to an M1 and M2 phenotype were used as controls at their respective concentrations. IL-10 neutralizing antibody (R&D Systems, MAB417, 0.8 μg/ml) was used to assess the overall effect of IL-10 on NPC proliferation and differentiation. Assessment of NPC proliferation (1 day post MCM treatment) and differentiation (7 days post MCM treatment) was accomplished as described above.

Dyck S., Kataria H., Alizadeh A., Santhosh K.T., Lang B., Silver J, & Karimi-Abdolrezaee S. (2018). Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury. Journal of Neuroinflammation, 15, 90.

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Stroke Core Tissue Isolation and Culture

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Mice were subjected to stroke surgeries as discussed above. 3 days after stroke, the visible infarct core regions from the mice were carefully dissected, pooled, and then dissociated in trypsin + EDTA. The pooled tissue suspension was centrifuged, washed with PBS, and resuspended with DMEM + 10% FBS, and plated on polystyrene tissue culture plates or into CS-A hydrogel. Following culture overnight, non-adherent cells and stroke core debris were removed, and the media was replaced. For the IL-10 neutralization experiments, 1 μg/μL anti-IL-10 antibody (R&D Systems, MAB417) was added to the cell culture media.

McCrary M.R., Jiang M.Q., Jesson K., Gu X., Logun M.T., Wu A., Gonsalves N., Karumbaiah L., Ping Yu S, & Wei L. (2022). Glycosaminoglycan scaffolding and neural progenitor cell transplantation promotes regenerative immunomodulation in the mouse ischemic brain. Experimental neurology, 357, 114177.

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Regulatory B Cell-Mediated T-Cell Suppression

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T-cell suppression assay by regulatory B cells was performed^{70 (link)}. Briefly, WT or slamf5^−/− B cells were first purified by B220⁺ magnetic beads and then transitional 2 (T2) B cells were isolated by sorting from spleens of mice in remission from EAE, and cultured 1:1 or 2:1 with WT or slamf5^−/− CD4⁺CD25⁻ T cells isolated by sorting for 72 h in the presence of 1 μg ml⁻¹ anti-CD3 (17A2; Biolegend) and 3 mg ml⁻¹ anti-CD28 (3751; Biolegend). To neutralize IL-10, cells were incubated with 10 μg ml⁻¹ of mouse IL-10 antibody (MAB417, R&D systems) or with IgG1-control (Biolegend).

Radomir L., Kramer M.P., Perpinial M., Schottlender N., Rabani S., David K., Wiener A., Lewinsky H., Becker-Herman S., Aharoni R., Milo R., Mauri C, & Shachar I. (2021). The survival and function of IL-10-producing regulatory B cells are negatively controlled by SLAMF5. Nature Communications, 12, 1893.

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Enteroid Treatment with IL-10 and Antibody

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Enteroids were treated with 1 μM 4OHT alone or together with 100 ng/mL mouse IL-10 (417-ML; R&D Systems). In some experiments, enteroids were co-cultured with splenocytes with or without rat anti-mouse IL-10 antibody (MAB417; R&D Systems).

Liang Z., He P., Han Y, & Yun C.C. (2022). Survival of Stem Cells and Progenitors in the Intestine Is Regulated by LPA5-Dependent Signaling. Cellular and Molecular Gastroenterology and Hepatology, 14(1), 129-150.

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Regulation of B10 Cell Differentiation by MDSCs

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Mononuclear cells separated from naive mouse spleen were cultured alone or with MDSCs in the presence or absence of 10 μg/ml lipopolysaccharides (LPS) (Sigma, No. L4391-1MG) for 72 h; the ratio of nonadherent PBMCs to MDSCs was 3:1. Mouse mononuclear cells isolated from naive mouse spleens by density gradient centrifugation were cultured alone or with MDSCs in the presence or absence of 10 μg/ml mouse BAFFR/TNFRSF13C antibody or mouse IL-10 antibody (R&D Systems, Minneapolis, MN, USA, AF1357; MAB417). Mononuclear cells separated from naive mouse spleen were cultured alone or with MDSCs in the presence or absence of 5 μM WP1066 (MCE, No. HY-15312) or 0.5 μM colivelin (MCE, No. HY-P1061).
Finally, the differentiation ratio of B10 cells induced by MDSCs was detected by FACS, and the phenotype of B10 cells was CD19⁺ CD1d⁺ CD5⁺.

Jianyi D., Haili G., Bo Y., Meiqin Y., Baoyou H., Haoran H., Fang L., Qingliang Z, & Lingfei H. (2022). Myeloid-derived suppressor cells cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer. Cancer Immunology, Immunotherapy, 72(1), 73-85.

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Continuous Cisterna Magna Administration of Compounds

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Mice were anesthetized with an IP injection of butorphanol (5.0 mg/kg), midazolam (4.0 mg/kg), and medetomidine (0.75 mg/kg). A small hole was made in the occipital bone after a small incision was made in the head skin, and a polyethylene tube (0.8 mm in diameter; Natsume, Tokyo, Japan) connected to an osmotic mini pump (0.5 µL/h, Alzet model 2002; Durect Corporation, Cupertino, CA, USA) was inserted into the cisterna magna through the hole [13 (link)]. Following this, 10602-R10N1 (500 μg/mL dissolved in 0.01 M PBS, 0.11 μL/h, Sino Biological, Beijing, China), MAB417 (500 μg/mL dissolved in 0.01 M PBS, 0.11 μL/h, R&D Systems, Minneapolis, MN, USA), or ab269199 (0.1 mg/mL, dissolved in 0.01 M PBS, 0.11 μL/h, Abcam, Cambridge, UK) was continually provided via ICM administration for 21 days after the left palatal mucosa incision via the osmotic mini pumps. MHWT was measured daily in each group as described above.

Ikutame D., Urata K., Oto T., Fujiwara S., Iinuma T., Shibuta I., Hayashi Y., Hitomi S., Iwata K, & Shinoda M. (2020). Aging-Related Phenotypic Conversion of Medullary Microglia Enhances Intraoral Incisional Pain Sensitivity. International Journal of Molecular Sciences, 21(21), 7871.

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