4 iodophenylboronic acid 4ipba

HT-29 human colon carcinoma was obtained from ATCC and maintained in DMEM containing 10% FBS (Omega Scientific), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids, and 100 units/ml penicillin and 100 μg/ml streptomycin. Diphenyliodonium (DPI), N-acetyl-L-cysteine (NAC), phorbol 12-myristate 13-acetate (PMA), horseradish peroxidase (HRP), luminol, H₂O₂, and 4-iodophenylboronic acid (4IPBA) were purchased from Sigma-Aldrich. Superoxide dismutase (SOD) was purchased from Abnova, and catalase (CAT) was purchased from MP Biomedicals.
FAK (Millipore), pY397 FAK (Invitrogen), pY402 Pyk2 (Cell Signaling), pY416 Src (Invitrogen), GAPDH (Millipore) antibody, and Alexa 488 phalloidin (Invitrogen) were obtained.

The Dionex UltiMate 3000 series UHPLC, C18 Acclaim PepMap NanoLC column (75 μm ID, 25 cm length C18), Fusion Lumos Q-Orbitrap-LTQ Tribrid MS (OIT) tandem mass spectrometer [36 (link)], LTQ XL linear ion trap (LIT) mass spectrometer [37 (link)] and Trizol reagent was from Thermo Fisher Scientific (Waltham, MA, USA). The 1100 HPLC was from Agilent (Santa Clara, CA, USA). Trypsin, salts, buffers, and luminol, H₂O₂, 4-iodophenylboronic acid (4IPBA) were obtained from Sigma Aldrich (St. Louis, MO, USA). The quaternary amine (QA) resin on a ceramic support was from BIORAD (Hercules, CA, USA). The HPLC grade water, ethanol, acetone and acetonitrile were obtained from Caledon Laboratories (Georgetown, Ontario, Canada). The 0.45 micron PVDF was from Millipore (Burlington, MA, USA). The imaging workstation was from BIORAD (Hercules, CA, USA).