In this study, melamine and cyanuric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA), with 99% purity. Pet food of one variety was obtained from a nearby animal hospital.
Cyanuric acid
Manufactured by Merck Group
Sourced in United States, Germany
Cyanuric acid is a chemical compound used in various industrial applications. It is a colorless, crystalline solid with the chemical formula C3H3N3O3. Cyanuric acid serves as a stabilizer and pH regulator in water treatment processes.
Automatically generated - may contain errors
Lab products found in correlation
Melamine, by Merck Group (11 mentions)
Rhodamine b rhb, by Merck Group (2 mentions)
4 methyl 5 vinylthiazole vta, by Merck Group (2 mentions)
2 4 diamino 6 phenyl 1 3 5 triazine, by Merck Group (2 mentions)
Isopropanol, by Merck Group (2 mentions)
Ammelide, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Ofloxacin, by Merck Group (1 mentions)
Ampicillin, by Merck Group (1 mentions)
Uracil, by Merck Group (1 mentions)
3 aminopropyltriethoxysilane, by Merck Group (1 mentions)
V 770 uv vis spectrophotometer, by Jasco (1 mentions)
Trisodium citrate dihydrate, by Kanto Chemical (1 mentions)
P nitroaniline pna, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
M phenylenediamine, by Merck Group (1 mentions)
Methyl alcohol, by Samchun (1 mentions)
Test tube, by Iwaki (1 mentions)
18 protocols using cyanuric acid
1
Melamine and Cyanuric Acid Analysis in Pet Food
2
Analytical-grade Chemicals for Research
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All chemicals used were of analytical-reagent grade. Melamine, cyanuric acid, ofloxacin, and ampicillin were purchased from Sigma-Aldrich (Darmstadt, Germany). Deionized water with the conductivity of 0.1 μS cm−1 was used for the preparation of all solutions and experiments.
3
Colorimetric Biosensor for Infant Formula Analysis
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Gold(Ⅲ) chloride trihydrate (≥99.9%), (3-Aminopropyl)-triethoxysilane (≥98.0%) (APTES), p-nitroaniline (p-NA), bovine serum albumin (BSA), cyanuric acid, urea, uracil, and m-phenylenediamine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trisodium citrate dihydrate was obtained from Kanto Chemical Co., Inc. (Tokyo, Japan). Methyl alcohol (99.5%) was provided by Samchun Pure Chemical Co., LTD. (Seoul, Korea). Glass substrate from Korea Testing & Research Institute (Gwacheon, Korea) were used. Infant formulas produced by Maeil Dairies Co., Ltd. (Seoul, Korea) were purchased at a local grocery store. Absorbance spectra were recorded on a V-770 UV-VIS spectrophotometer (JASCO International Co., Ltd., Tokyo, Japan), and field emission scanning electron microscopy (FE–SEM) measurements were conducted on a JITACHI S-4300 system.
4
Colorimetric Assay for Heavy Metal Detection
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UV–Vis spectrophotometry (Thermo Scientific, Waltham, MA, USA), pH meter (Eutech, Breda, The Netherlands), oven (Memmert, Schwabach, Germany) micropipette (DLAB, Beijing, China), beaker (Iwaki, Japan), Erlenmeyer (Iwaki, Japan), test tube (Iwaki, Japan), volumetric flask (Iwaki, Japan), stir bar, vacuum filter, volume pipette (Pyrex, Shizuoka, Japan), analytical balance (Sartorius, Göttingen, Germany), ultrasonic stirrer (Branson, MO, USA), Whatman No. 1, Whatman No. 41, Whatman No. 91, Whatman grade 1 chromatography paper, 0.45 µm membrane, and ImageJ software (National Institutes of Health, Bethesda, MD, USA).
AuNPs (10 nm diameter, OD 1) (Sigma Aldrich, St. Louis, MO, USA), cyanuric acid (Sigma Aldrich), ethanol (Merck, Darmstadt, Germany), double distilled H2O (Ikapharmindo Putramas, East Jakarta, Indonesia), HgCl2 (Smartlab, Semarang, Indonesia), MgSO4, ZnCl2, AgNO3, FeSO4, CdSO4, BaCl2, and MnCl2 (Merck, Germany), NaCl (Merck, Germany), Tris-HCl (Himedia, Kennett Square, PA, USA), as well as water samples from Kenanga Lake, FMIPA Lake, and Mahoni Lake.
AuNPs (10 nm diameter, OD 1) (Sigma Aldrich, St. Louis, MO, USA), cyanuric acid (Sigma Aldrich), ethanol (Merck, Darmstadt, Germany), double distilled H2O (Ikapharmindo Putramas, East Jakarta, Indonesia), HgCl2 (Smartlab, Semarang, Indonesia), MgSO4, ZnCl2, AgNO3, FeSO4, CdSO4, BaCl2, and MnCl2 (Merck, Germany), NaCl (Merck, Germany), Tris-HCl (Himedia, Kennett Square, PA, USA), as well as water samples from Kenanga Lake, FMIPA Lake, and Mahoni Lake.
5
Synthesis and Characterization of g-CN
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Al2O3 basic (Sigma-Aldrich),
citric acid (ACS grade 99.5%, Sigma-Aldrich), cyanuric acid (98%,
Sigma-Aldrich), D2O (99.9%, Sigma-Aldrich), dichloromethane
(DCM, anhydrous HPLC grade, Merck), ethylene glycol (EG, 99%, Fluka),
hydrochloric acid (1 M solution, Merck), hydroquinone (ReagentPlus,
Sigma-Aldrich), melamine (99%, Sigma-Aldrich), potassium hydroxide
(Sigma-Aldrich), rhodamine B (RhB, >95% Sigma-Aldrich), and tetrahydrofuran
(THF, anhydrous, Sigma-Aldrich) were used as purchased. 2-HEMA (99%,
Sigma-Aldrich), N,N-dimethylacrylamide
(DMA, 99%, Sigma-Aldrich), and styrene (ReagentPlus, Sigma-Aldrich)
were passed through basic alumina column prior to use to remove the
inhibitor. For visible light irradiation, 50 W LED chips (Foxpic High
Power 50 W LED Chip Bulb Light DIY White 3800LM 6500 K) were connected
to a self-made circuit and cooling system. g-CN from the cyanuric
acid-melamine complex (noted as CM) was synthesized according to the
literature.62 (link)
citric acid (ACS grade 99.5%, Sigma-Aldrich), cyanuric acid (98%,
Sigma-Aldrich), D2O (99.9%, Sigma-Aldrich), dichloromethane
(DCM, anhydrous HPLC grade, Merck), ethylene glycol (EG, 99%, Fluka),
hydrochloric acid (1 M solution, Merck), hydroquinone (ReagentPlus,
Sigma-Aldrich), melamine (99%, Sigma-Aldrich), potassium hydroxide
(Sigma-Aldrich), rhodamine B (RhB, >95% Sigma-Aldrich), and tetrahydrofuran
(THF, anhydrous, Sigma-Aldrich) were used as purchased. 2-HEMA (99%,
Sigma-Aldrich), N,N-dimethylacrylamide
(DMA, 99%, Sigma-Aldrich), and styrene (ReagentPlus, Sigma-Aldrich)
were passed through basic alumina column prior to use to remove the
inhibitor. For visible light irradiation, 50 W LED chips (Foxpic High
Power 50 W LED Chip Bulb Light DIY White 3800LM 6500 K) were connected
to a self-made circuit and cooling system. g-CN from the cyanuric
acid-melamine complex (noted as CM) was synthesized according to the
literature.62 (link)
6
Synthesis and Characterization of Triazine Compounds
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2,4-diamino-6-phenyl-1,3,5-triazine (97%, Sigma Aldrich, Darmstadt, Germany), 4-methyl-5-vinylthiazole (vTA, 97%, Sigma Aldrich), anthracene (>96%, Sigma Aldrich, Germany), chloroform (99%, Merck, Germany), cyanuric acid (98%, Sigma Aldrich), ferrocene (98%, Sigma Aldrich, Germany), poly(ethylene imine) (PEI, Mn 60,000 g/mol, 50 wt% in water, Sigma Aldrich), pyrene (98%, Sigma Aldrich, Germany).
7
Synthesis and Characterization of PA6
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PA6
(1034B) was supplied
by Ube Industries Ltd., Tokyo, Japan. HCCP, BPS, TEA, acetonitrile
98%, DMSO, melamine, and cyanuric acid were purchased from Sigma-Aldrich,
Johannesburg, South Africa, and used as received without further purification.
(1034B) was supplied
by Ube Industries Ltd., Tokyo, Japan. HCCP, BPS, TEA, acetonitrile
98%, DMSO, melamine, and cyanuric acid were purchased from Sigma-Aldrich,
Johannesburg, South Africa, and used as received without further purification.
8
Cyanuric Acid and Atrazine Exposure Protocols
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Stock solutions of cyanuric acid and atrazine (both from Sigma-Aldrich, Fr, purity ≥98%) were made in isopropanol/ultrapure water (20/80 v/v) at concentrations of 2.5 mM and 150 μM, respectively. These solutions were stocked at −20°C for 3 months and diluted in ultrapure water to required concentrations prior to use. Because solubility thresholds differ from cyanuric acid and atrazine (21 mM and 161 μM, respectively), the concentration ranges used for exposure were between 4 nM and 4 mM and between 8 nM and 15 μM, respectively.
9
Hydrogel Synthesis from Agar, Cyanuric Acid, and Melamine
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Agar (A7921; powder; CAS
9002-18-0), cyanuric
acid 98% (1,3,5-triazine-2,4,6-triol; (CNOH)3; 129.07 g
mol–1; Sigma-Aldrich, CAS 108-80-5), melamine (1,3,5-triazine-2,4,6-triamine;
C3(HN)6; 126.12 g mol–1; Sigma-Aldrich,
CAS 108-78-1), sodium hydroxide (NaOH; 39.997 g mol–1; Lenreaktiv, 130109 LR), hydrochloric acid (HCl; 36.46 g mol–1; Lenreaktiv), sodium fluorescein (C20H10Na2O5; 376.27 g mol–1), and peptone-yeast MRS medium were used. Distilled water from Millipore
Elix (18 MΩ·cm2/cm) was used to prepare the
solutions for all experiments.
9002-18-0), cyanuric
acid 98% (1,3,5-triazine-2,4,6-triol; (CNOH)3; 129.07 g
mol–1; Sigma-Aldrich, CAS 108-80-5), melamine (1,3,5-triazine-2,4,6-triamine;
C3(HN)6; 126.12 g mol–1; Sigma-Aldrich,
CAS 108-78-1), sodium hydroxide (NaOH; 39.997 g mol–1; Lenreaktiv, 130109 LR), hydrochloric acid (HCl; 36.46 g mol–1; Lenreaktiv), sodium fluorescein (C20H10Na2O5; 376.27 g mol–1), and peptone-yeast MRS medium were used. Distilled water from Millipore
Elix (18 MΩ·cm2/cm) was used to prepare the
solutions for all experiments.
10
Protein Quantification and Purification
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Sucrose, Tris-HCl, EDTA, NaOH, HCl, NaCl, isopropanol, glycine, cyanuric acid, 2,4,6-trinitrobenzenesulphonic acid (TNBS), 2-methylbutylamine, 4-aminobenzoic acid, sodium citrate, bicinchoninic acid (BCA), bovine serum albumin (BSA), p-nitrophenylbutyrate (p-NPB), acetonitrile, Sodium dodecyl sulfate, acrylamide and bia-acrylamide, ethanol, acetic acid, sodium thiosulfate, silver nitrate, formaldehyde, and sodium carbonate were purchased from Sigma-Aldrich (Darmstadt, Germany). BCATM (bicinchoninic acid) and Micro BCATM Protein Assays Reagents were from ThermoScientific (Rockford, IL, USA). Sepharose CL-6B was purchased from Sigma-Aldrich. The anion-exchange chromatography (DEAE-cellulose and Q-Sepharose) columns used were from GE Healthcare (Amersham, UK).
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