Eriodictyol 7 o glucoside

All solvents used (methanol, acetonitrile, acetic acid, water and ethyl acetate) were HPLC-grade and were purchased from Merck (Darmstadt, Germany). Protocatechuic acid, 4-hydroxybenzoic acid, vanillic acid, chlorogenic acid, trans-p-coumaric acid, eriodictyol-7-O-glucoside, eriodictyol, naringenin, naringenin-7-O-glucoside, kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside, isorhamnetin-3-O-glucoside, quercetin, kaempferol, isorhamnetin, quercetin-3-O-rutinoside, quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-rutinoside, catechin and epicatechin were purchased from Extrasynthese (Genay, France). Other chemicals were of analytical grade.

Rosmarinic acid, carvacrol, luteolin-7-O-glucoside, kaempferol, apigenin-7-O-glucoside, and eriodictyol-7-O-glucoside were purchased from Extrasynthese (Genay, France). Methanol, n-hexane, and acetonitrile with HPLC purity were obtained from Lab-Scan (Lisbon, Portugal). Water was treated in a Direct-Q^® water purification system (Merck Life Science, Darmstadt, Germany). Folin-Ciocalteu reagent was obtained from Panreac (Barcelona, Spain), sodium carbonate (Na₂CO₃), aluminum chloride (AlCl₃), gallic acid, rutin, 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), butylated hydroxytoluene (BHT), lyophilized acetylcholinesterase (AChE, from electric eel, type VI-S), Iodure acetylthiocholine, DTNB 5,5′-dithio-bis-[2-nitrobenzoic acid] (DTNB), and Donepezil were purchased from Sigma-Aldrich (St. Louis, MO, USA). The 2,4,6-tripyridyls-triazine (TPTZ) and β-carotene were obtained from Fluka (Munich, Germany). Tryptic soy broth was purchased from Scharlau Microbiology (Barcelona, Spain).

Authentic phenolic standards of chlorogenic acid, catechin, epicatechin, quercetin-3-O-galactoside, rutin, quercetin-3-O-glucoside, quercetin-3-O-xyloside, quercetin-3-O-arabinoside, quercetin-3-O-rhamnoside, isorhamnetin-3-O-glucoside, quercetin, kaempferol, eriodictyol-7-O-glucoside, cyanidin-3-O-galactoside, cyanidin-3-O-arabinoside, cyanidin-3-O-rhamnoside, and phloridzin were purchased from Extrasynthese (Genay, France) or Sigma-Aldrich (St. Louis, MO, USA). Sugars (glucose, fructose, sucrose, and sorbitol) and organic acids (citric acid, malic acid, and shikimic acid) were also purchased from Sigma-Aldrich. Acetonitrile (HPLC-grade) was obtained from J.T. Baker (Phillipsburg, NJ, USA). Nanopure water used in the study was obtained from a water purification system (Milli-Q Direct 8, Merck Millipore, Billerica, MA, USA).