The human pancreatic tumor cell line PANC-1 was obtained from the European Collection of Cell Cultures (ECACC, Sigma Aldrich). Authentication of PANC-1 cell line was performed by ECACC. Their testing regime includes Short Tandem Repeat (STR) profiling and standardized PCR-based authentication methods. Moreover, cells were not passaged for more than 6 months. PANC-1 cells were maintained as monolayer cultures in RPMI-1640 (Lonza, #12–702) supplemented with 10% FCS (Sigma-Aldrich, #F7524), 100 units/ml of streptomycin, 100 μg/ml of penicillin (Invitrogen, #15140–122) and 2 mM Glutamine (Lonza, #17-605E). Cultures were grown at 37°C in a humidified atmosphere of 5% CO2. Regular testing of the presence of mycoplasma was performed with the use of a commercial kit (Lonza, #LT-518).
Panc 1
Manufactured by Lonza
Sourced in Switzerland, Belgium
PANC-1 is a cell line derived from a human pancreatic carcinoma. It is a commonly used in vitro model for the study of pancreatic cancer biology and drug discovery research.
Automatically generated - may contain errors
Lab products found in correlation
Fetal calf serum (fcs), by Merck Group (3 mentions)
Panc 1, by European Collection of Cell Cultures (3 mentions)
Rpmi 1640, by Lonza (3 mentions)
Glutamine, by Lonza (3 mentions)
Panc 1, by American Type Culture Collection (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Mia paca 2, by American Type Culture Collection (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Mia paca 2, by Lonza (2 mentions)
Dulbecco modified eagle medium (dmem), by Lonza (2 mentions)
Ecacc cell lines, by Merck Group (2 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Mycoalert detection kit, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
Rpmi 1640, by Fujifilm (1 mentions)
Panc 1, by Fujifilm (1 mentions)
Amphotericin b, by Nacalai Tesque (1 mentions)
9 protocols using panc 1
1
Authentication and Maintenance of PANC-1 Cells
2
Culturing Human Pancreatic Cancer Cells
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Human pancreatic cancer cells, PANC-1, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). PANC-1 cells were confirmed to be mycoplasma-free using a Mycoalert detection kit (Lonza, Basel, Switzerland). PANC-1 cells were cultured in RPMI 1640 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), supplemented with 10% (v/v) fetal bovine serum (FBS, Biowest, Nuaillé, France) and antibiotics (100 U/mL penicillin, 100 mg/mL streptomycin, and 0.25 mg/mL amphotericin B, Nacalai Tesque, Kyoto, Japan), at 37 °C in a humidified atmosphere with 5% CO2 and 20% O2.
3
Comprehensive Cell Line Validation Protocol
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All the cell lines used in this study were obtained from Holding Company for Biological Products and Vaccines VACSERA, Egypt; MCF-7 cells (Human breast adenocarcinoma), HepG2 (human hepatocellular carcinoma), Caco-2 (colon carcinoma), A549 (human lung adenocarcinoma), PANC-1 (human pancreatic cancer), Vero cells (derived from normal kidney cells).
The RPMI 1640 medium (Lonza, Switzerland) was used to maintain all cell lines. The media also contained 10% fetal bovine serum (Gibco, USA), 1% penicillin, and 1% streptomycin (Sigma Aldrich, USA). In a humidified cell incubator with a 5% Carbon dioxide environment, the cells were kept at 37 °C. The study was examined and approved by the Ethics committee in the Faculty of Pharmacy-October University for Modern Sciences and Arts (MSA) with ethics approval number (BP2/Ec2/2021PD).
The RPMI 1640 medium (Lonza, Switzerland) was used to maintain all cell lines. The media also contained 10% fetal bovine serum (Gibco, USA), 1% penicillin, and 1% streptomycin (Sigma Aldrich, USA). In a humidified cell incubator with a 5% Carbon dioxide environment, the cells were kept at 37 °C. The study was examined and approved by the Ethics committee in the Faculty of Pharmacy-October University for Modern Sciences and Arts (MSA) with ethics approval number (BP2/Ec2/2021PD).
4
Culturing Pancreatic Cancer Cell Lines
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The certified human caucasian PDAC cell lines MIA-PaCa-2, PANC-1, BxPC-3, SU.86.86, and AsPC-1 were purchased from the American Type Culture Collection (ATCC; Rockville, MD). The cell lines carry TP53 missense mutations, all sequenced and validated by ATCC.
PANC-1 and MIA-PaCa-2 cells were cultured in phenol red free Dulbecco's Modified Eagle Medium (DMEM; Lonza, Walkersville, USA) supplemented with 10% heat inactivated fetal calf serum (FCS; Gibco, Carlsbad, USA), 2mM L-glutamine (Gibco), and 1mM sodium pyruvate (Sigma, St. Louis, USA) in a humidified incubator containing 95% air and 5% CO2 at 37°C. The medium for MIA-PaCa-2 cells was also supplemented by 2% heat inactivated horse serum (Gibco). BxPC-3, SU.86.86, and AsPC-1 cells were cultured under the same conditions in phenol red free RPMI-1640 medium (Gibco) containing 10% heat inactivated FCS, 2mM L-glutamine, 1mM sodium pyruvate, and 4.5g/l glucose (Sigma). All cell culture experiments were carried out without antibiotics.
PANC-1 and MIA-PaCa-2 cells were cultured in phenol red free Dulbecco's Modified Eagle Medium (DMEM; Lonza, Walkersville, USA) supplemented with 10% heat inactivated fetal calf serum (FCS; Gibco, Carlsbad, USA), 2mM L-glutamine (Gibco), and 1mM sodium pyruvate (Sigma, St. Louis, USA) in a humidified incubator containing 95% air and 5% CO2 at 37°C. The medium for MIA-PaCa-2 cells was also supplemented by 2% heat inactivated horse serum (Gibco). BxPC-3, SU.86.86, and AsPC-1 cells were cultured under the same conditions in phenol red free RPMI-1640 medium (Gibco) containing 10% heat inactivated FCS, 2mM L-glutamine, 1mM sodium pyruvate, and 4.5g/l glucose (Sigma). All cell culture experiments were carried out without antibiotics.
5
Detailed Characterization of Pancreatic Cell Lines
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CFPAC-1 (ECACC, UK) was derived from a liver metastasis of a well-differentiated PDAC in a 26-year-old male [15 (link)]. PANC-1 (RIKEN BRC, Japan) was derived from a poorly-differentiated PDAC in a 56-year-old female [15 (link)]. H6c7 (Kerafast, USA) is an immortalised pancreatic ductal cell line. Derived from a 51-year-old female who underwent pancreaticoduodenectomy but whose histology showed no evidence of malignancy, it has a near-normal phenotype and genotype and is considered to represent normal pancreatic ductal cells [16 (link),17 (link)]. CFPAC-1 was cultured in Iscove's modified Dulbecco's medium with l -glutamine, HEPES buffer (Gibco™) and 10% foetal bovine serum (FBS) (Gibco™). PANC-1 was cultured in Dulbecco's modified Eagle's medium with glucose, l -glutamine (Lonza®) and FBS. H6c7 was cultured in Keratinocyte FBS-free media with l -glutamine, epidermal growth factor and bovine pituitary extract (Gibco™) with 1% antibiotic/antimycotic (Gibco™). Cells were cultured in 75 cm2 tissue culture flasks in a humidified incubator at 37.0 °C with 5% CO2 and passaged when ~80% confluent.
6
Culturing Human and Murine Cancer Cells
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Human pancreatic carcinoma cell line PANC1 (ATCC), murine fibrosarcoma cell line MN/MCA1, and murine lung adenocarcinoma cell line CMT167 (ECACC) were cultured in DMEM (Lonza) with 10% FBS and 0.5% penicillin/streptomycin (Gibco) at 37°C and 5% CO2.
7
Characterization of Cancer Cell Lines
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U2OS, PANC-1, NUGC-3, SW620, and MIA PaCa-2 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). The BJ-hTER and BJ-RasV12 cell were provided by W. Hahn (Dana-Farber Cancer Institute) and the DR-GFP U2OS cells were provided by M. Jasin (Memorial Sloan Kettering Cancer Center). U2OS, DR-GFP U2OS, BJ-hTERT, and BJ-RasV12 cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% fetal bovine serum (FBS, Life Technologies/ThermoFisher Scientific), and penicillin–streptomycin antibiotics (50 U/mL). MIA PaCa-2, PANC1, SW620, and SW480 were grown in DMEM (Lonza Biosciences), with 10% FBS (Life Technologies/ThermoFisher Scientific). Cells were grown at 37 °C in 5% CO2 humidified incubators.
8
Culturing Pancreatic Tumor Cell Line PANC-1
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The human pancreatic tumour cell line PANC-1 was obtained from the European Collection of Cell Cultures (ECACC Cell Lines, Sigma Aldrich). PANC-1 cells were maintained as monolayer culture in RPMI-1640 (Lonza, Verviers, Belgium) with 10% FCS (Sigma-Aldrich, St. Louis USA), 1% streptomycin/penicillin (Invitrogen, USA) and 2 mM glutamine (Lonza,, Verviers, Belgium).
Cultures were grown at 37 °C in a humidified atmosphere of 5% CO2/95% air. Regular testing of the presence of Mycoplasma was performed with the use of a commercial kit (Lonza, Rockland, ME, USA).
Cultures were grown at 37 °C in a humidified atmosphere of 5% CO2/95% air. Regular testing of the presence of Mycoplasma was performed with the use of a commercial kit (Lonza, Rockland, ME, USA).
9
Culturing Human Pancreatic Tumor Cells
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