N hydroxysuccinimide nhs

Polyvinyl alcohol (PVA, CAS: 9002-89-5, alcoholysis: 97.5–99 mol%, viscosity: 3.5–4.5 mPa⋅s) and N,N-diethylethylenediamine (DEEDA, CAS: 111-74-0) were purchased from Shanghai Aladdin Biochemistry Technology Co., Ltd. (Shanghai, China). Hyaluronic acid (HA, CAS: 9067-32-7, molecular weight: 150,000-250,000) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Antimicrobial peptide (AMP, KRWWKWWRRC, purity>95%) was obtained from Shanghai Apeptide Co., Ltd. (Shanghai, China). Tetrakis (4-bromomethylphenyl) ethylene (TPE, CAS: 67448-47-9, purity: 90%) was purchased from Zhengzhou Alpha Chemical Co., Ltd. (Zhengzhou, China). Sodium alginate (ALG, CAS: 9005-38-3, viscosity: 350–550 mPa⋅s^-1), N,N′-Carbonyldiimidazole (CDI, CAS:530-62-1, purity: 99%), N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, CAS: 25952-53-8, purity: 98.5%) and N-hydroxysuccinimide (NHS, CAS: 6066-82-6, purity: 98%) were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Silkworm cocoon was obtained from Chinese Academy of Agricultural Sciences. All other chemical reagents were analytically pure and purchased from Shanghai Aladdin Biochemistry Technology Co., Ltd. (Shanghai, China).

Chloroauric acid (HAuCl₄) and sodium citrate were purchased from Shanghai Aladdin Biochemical Technology Co. Ltd. Carboxyl-modified quantum dots (-COOH QDs) were obtained from Suzhou Xingshuo Nanotechnology Co. Ltd. 1-ethyl-(3-dimethylaminopropyl) carboimide (EDC) and N-hydroxysuccinimide (NHS) were purchased from Shanghai Macklin Biochemical Co. Ltd. The amino-modified CD133 aptamer (5′-NH₂-CCCUCCUACAUAGGG-3′) and CD133 aptamer partial complementary pairing part (ssRNA, 5′-SH-CCCUAUG-3′) were synthesized by Shanghai Generay Biotech Co. Ltd. All buffers were prepared with ultrapure water, which was purified to a resistivity of 18.2 MΩ*cm by a milli-Q system (Merck Millipore, United States). A dynamic light scattering particle size analyzer (SZ-100, Horiba, Japan) was used for measuring the zeta potential and particle size. The morphology of nanoparticles was characterized by transmission electron microscopy (JEOL JEM-2100 F, JEOL Ltd., Japan). The UV–Vis absorption spectra were recorded on a microspectrophotometer (NanoDrop ND-1000, Thermo Scientific, United States). Fluorescence spectra were obtained using a fluorescence spectrophotometer (F-7000, Hitachi, Japan). Fluorescence images were recorded using a confocal laser scanning microscope (TCS SP8 Leica, United States).

CS (deacetylated degree: 90%; average molecular weight: 1.5 × 10⁵ Da), ferulic acid, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) and 1,1,3,3-tetramethoxypropane were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). All other reagents were of analytical grade.

NheI and SpeI were obtained from New England Biolabs. Nickel nitrilotiacetic acid resin was obtained from Qiagen. 1-dodecanethiol (DT, 98%), diethyldithiocarbamic acid silver salt (98%), trichlormethane, acetone, dimethyl sulfoxide (DMSO), ampicillin, and kanamycin were purchased from Sinopharm Group Chemical Reagent Co. Ltd. Chlorin e6 (Ce6) was obtained from Frontier Scientific Inc., and 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[amine methoxy (polyethylene glycol)₂₀₀₀] (DSPE-mPEG₂₀₀₀-NH₂) was purchased from Avanti. Ultrapure water (Millipore Mill-Q grade, 18.2 MΩ) was used in all the experiments. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) were obtained from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). DMEM/F12 and RPMI 1640 were purchased from Thermo Fisher Scientific. Staphylococcus aureus’ species-identifiable aptamer was obtained from Tsingke Biotechnology Co., Ltd. All reagents and solvents are of analytical grade and used without further purification.

OA was distilled from Camellia seed oil in our lab with its purity of 98% [31 (link),32 (link)]. PEI with molecular weight ≈10,000, mPEG with molecular weight ≈2000, DOX, succinic anhydride, N-hydroxysuccinimide (NHS), 4-Nitrophenyl Chloroformate, o-nitrobenzyl alcohol, 4-dimethylaminopyridine (DMAP), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were purchased from Macklin Reagent Co. (Shanghai, China). Yttrium oxide (Y₂O₃, 99.99%), ytterbium oxide (Yb₂O₃, 99.99%), erbium oxide (Er₂O₃, 99.99%), and sodium fluoride (NaF, 98%) were purchased from Shanghai Chemical Industrial Company (Shanghai, China). Pyrene, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenytetrazolium bromide (MTT), and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Aladdin Reagent Co. (Shanghai, China). Phosphotungstic acid dye, glutaraldehyde, and copper mesh were purchased from Beijing Zhongjingkeyi Technology Co. (Beijing, China). Human lung adenocarcinoma cells A549 cells and DMEM medium were bought from Shanghai Cell Bank (Shanghai, China). Chloroform, acetonitrile, ethyl ether, and dimethyl sulfoxide (DMSO) were supplied by Sinopharm Chemical Reagent Co. (Shanghai, China). Transparent polystyrene 6-well and 96-well plates were obtained from Corning Co. (Corning, NY, USA).