The following stains/antibodies were used for surface staining: BD Horizon™ Fixable Viability Stain 450 (BD Biosciences, San Jose, CA, USA), CD45-V500 (clone HI30, BD Biosciences), CD14- APC-Cy7 (clone M5E2, BioLegend, San Diego, CA, USA), CD117-APC (clone 104D2, BD Biosciences), FcεRIα-PE and FcεRIα-FITC (clone AER-37 (CRA-1), BioLegend), CD34-Pe-Cy7 (clone 581, BD Biosciences), Integrin-β7-FITC (clone FIB504, eBioscience), MrgX2-PE (clone K125H4, BioLegend), and CD63-Pe-Cy7 (clone H5C6, BD Biosciences). To measure proliferation, the cells were labeled with CellTrace™ Far Red (Thermo Fisher Scientific) prior to treatment. Pure human lung mast cells were obtained by FACS of CD45+CD14−CD117high cells using a FACSAria I instrument, flow cytometric analyses were performed with a FACSCanto II instrument (BD, Franklin Lakes, NJ, USA), and flow cytometry data analysis was performed with FlowJo software version 10 (FlowJo LLC, Ashland, OR, USA).
Cd34 pe cy7 clone 581
Manufactured by BD
Sourced in United States
CD34-Pe-Cy7 (clone 581) is a fluorescently labeled monoclonal antibody targeting the CD34 antigen. It is designed for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Horizon fixable viability stain 450, by BD (1 mentions)
Cd45 v500 clone hi30, by BD (1 mentions)
Cd14 apc cy7 clone m5e2, by BioLegend (1 mentions)
Integrin β7 fitc clone fib504, by Thermo Fisher Scientific (1 mentions)
Celltrace far red, by Thermo Fisher Scientific (1 mentions)
Facs canto 2 instrument, by BD (1 mentions)
Software version 10, by FlowJo (1 mentions)
Cd63 pecy7, by BD (1 mentions)
Cd66b clone g10f5, by BD (1 mentions)
Superfrost plus microscope slide, by Thermo Fisher Scientific (1 mentions)
Apc cd33 clone wm53, by BD (1 mentions)
Facscanto flow cytometer, by BD (1 mentions)
Ba310, by Motic (1 mentions)
2 protocols using cd34 pe cy7 clone 581
1
Characterization of Human Lung Mast Cells
2
Characterization of Neutrophil Functionality
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At the end of granulopoietic differentiation, cells were cytospun onto a Superfrost Plus Microscope slide (Fisherbrand, ThermoFisher Scientific, Waltham, MA). The slides were Wright–Giemsa stained and scored for myeloid cell types (promyelocytes, myelocytes, metamyelocytes, bands, neutrophils, and monocytes) using an upright microscope (Motic BA310). For the immunophenotypic characterization of neutrophils, cells were stained for CD45‐Pacific Blue (clone HI30, catalog #560367), CD34‐PECy7 (clone 581, catalog #561440), CD33‐APC (clone WM53, catalog #551378, CD11b‐5APCCy7 (clone ICRF44, catalog #557754, clone ICRF44), and CD16‐PE (clone W6D3, catalog #562370), and CD66b (clone G10F5, catalog #561927) from BD Biosciences. The neutrophil population (defined as CD45+/CD34−/CD14−/CD11b+/CD16+) was gated and used for analysis of ROS generation, bacterial phagocytosis and phospho‐FACS in a FACS‐Canto flow cytometer (BD Biosciences).
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