Polycaprolactone (Mw = 160000) was purchased from Jinan Daigang Biomaterial Co., Ltd, Jinan, China. Gelatin powder (type A) (Mw = 80000) was obtained from Sigma-Aldrich Co., Ltd, China. MgO nanoparticles were purchased from Shanghai Bike New Material Technology Co., Ltd, China. 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) was purchased from Aladdin Co., Ltd, China. Cell-LightTM EdU DNA Cell Proliferation Kit was obtained from RiboBio Co., Ltd, China. Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Co., Ltd, Japan.
Gelatin powder type a
Manufactured by Merck Group
Sourced in United States
Gelatin powder (type A) is a fine, light-colored powder derived from the partial hydrolysis of collagen. It is a natural, edible, and versatile ingredient used in various applications. The core function of gelatin powder is to act as a gelling agent, thickening agent, and stabilizer in food, pharmaceutical, and other industries.
Automatically generated - may contain errors
Lab products found in correlation
Cd146, by BioLegend (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Cell light edu dna cell proliferation kit, by RiboBio (1 mentions)
Sodium alginate powder, by Merck Group (1 mentions)
α minimum essential medium α mem, by Thermo Fisher Scientific (1 mentions)
Tripolyphosphate tpp, by Merck Group (1 mentions)
Hsa mir 21 mimics, by GenePharma (1 mentions)
Stro 1, by BD (1 mentions)
Stro 1, by BioLegend (1 mentions)
4 protocols using gelatin powder type a
1
Polycaprolactone-Gelatin Nanocomposite Scaffold
2
Gelatin-Alginate-Collagen Bioink for HCEC
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Gelatin powder (type A; Sigma) and sodium alginate powder (Sigma) were dissolved in phosphate-buffered saline as described in previous studies36 (link)37 (link). A gelatin/alginate solution was added to a neutralised rat-tail type I collagen (First link) solution with final concentrations of 1% (w/v) alginate, 10% (w/v) gelatin and 0.513, 0.615, 0.82, and 1.025 mg/ml collagen prior to the printing process. HCECs were collected by centrifugation at 1500 rpm for 3 min. The cells were dissociated into single cells and then gently mixed with the gelatin-alginate-collagen solution to reach a final concentration of 106 cells/ml.
3
Chitosan-Hyaluronic Acid Nanoparticles for miRNA Delivery
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CS was obtained from Jinke Co. Ltd (Zhejiang, People’s Republic of China), with a molecular weight of 100 kDa and a deacetylation degree of 90%. HA, with a molecular weight of 10 kDa, was purchased from CP Freda Pharmaceutical Co., Ltd (Shangdong, People’s Republic of China). Tripolyphosphate (TPP) was supplied by Sigma-Aldrich Co. (St Louis, MO, USA). The hsa-miR-21 mimics (5′-uagcuuaucagacugauguugadTdT-3′; 5′-ucaacaucagucugauaagcuadTdT-3′) were purchased from GenePharma (Shanghai, People’s Republic of China). Gelatin powder (type A) and vitamin C were purchased from Sigma-Aldrich Co. α-minimum essential medium (α-MEM) and fetal bovine serum were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Fluorescently labeled monoclonal antibodies, including STRO-1, were acquired from BD Biosciences (San Jose, CA, USA); CD146, CD29, CD34, and CD45 were purchased from Biolegend (San Diego, CA, USA). Primers for real-time polymerase chain reaction (PCR) were purchased from Sangon Biotechnology Co. (Shanghai, People’s Republic of China). Antibodies against collagen type Iα1 (COL-I), runt-related transcription factor 2 (RUNX2), osteopontin (OPN), and osteocalcin (OCN) were all provided by Santa Cruz Biotechnology Inc. (Dallas, TX, USA).
4
Fabrication of Chitosan-Hyaluronic Acid Scaffolds
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CS with a molecular weight of 100 kDa and a deacetylation degree of 90% was purchased from JINKE Company Limited (Zhejiang, People’s Republic of China). HA, with a molecular weight of 10 kDa, was obtained from Shandong C. P. Freda Pharmaceutical Co., Ltd. (Shandong, People’s Republic of China). The miR-21 and negative control oligonucleotides were purchased from Shanghai GenePharma Co, Ltd. (Shanghai, People’s Republic of China). Gelatin powder (type A) was purchased from Sigma-Aldrich (St Louis, MO, USA). Pure Ti (1.5 mm in thickness and 20 mm in diameter) was purchased from the Northwest Institute for Nonferrous Metal Research (Xi’an, People’s Republic of China). Alpha Minimum Essential Medium (α-MEM) and fetal bovine serum were obtained from HyClone (Thermo Fisher Scientific, Waltham, MA, USA). Fluorescently labeled monoclonal antibodies, including STRO-1, CD146, CD34, and CD45, were purchased from BioLegend (San Diego, CA, USA).
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