Cells (4 × 105/mL) were incubated with 150 μM capsaicin or vehicle for 5 h, and the activities of caspase-3/7, -8, and -9 were measured using the Caspase-Glo Assay with a luciferin-conjugated polypeptide substrate (Promega, Madison, WI, USA) (13 (link)). Luminescence was measured on a luminescence microplate reader (Tecan M200). The caspase activities of untreated cells were defined as 1.0 relative light unit.
Luciferin conjugated polypeptide substrate
Manufactured by Promega
Sourced in United States
The Luciferin-conjugated polypeptide substrate is a laboratory reagent that contains a luciferin molecule chemically coupled to a short polypeptide sequence. This substrate is designed to be used in biochemical assays that measure the activity of enzymes that can cleave the polypeptide, thereby releasing the luciferin for detection.
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Lab products found in correlation
2 protocols using luciferin conjugated polypeptide substrate
1
Capsaicin-Induced Caspase Activity
2
Caspase-3/7 Activity in PEL Cells
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The cells (4×105 cells/ml) were incubated with 20 µM of derivative #5 for 8 h, and caspase-3/7 activities were measured using the Caspase-Glo Assay with a luciferin-conjugated polypeptide substrate (Promega Corporation). In accordance with a previous study by the authors, the activation of caspases in PEL cells was detected at 6-12 h following the addition of anticancer drugs (26 (link)), and the drug-treatment time was set at 8 h. The luminescence was measured on a Tecan M200 luminescence microplate reader (Tecan Group, Ltd.). The caspase activities of the untreated cells were defined as 1.0.
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