Rosiglitazone (RSG # A00183, MW: 357.4) was obtained from the Adipogen. Reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-Rad Laboratories (Hercules, CA, USA). The antibodies used in this study were purchased from the various sources: Rabbit anti-ZO-1 (#402200), mouse anti-occludin (#331500) and anti-claudin-5 (#4C3C2) from Life Technologies; rabbit anti-PPARγ (#331500) from Invitrogen; rabbit anti-Nrf2 (#NBP-1-32822) from Novus Biologicals. Mouse anti-PECAM-1 (#sc-376764), mouse anti-NQO-1 (#sc-376023), mouse anti-HO1 (#sc-390991) and mouse anti-NFκB-p65 (#sc-(F-6)-8008) from Santa Cruz Biotechnology. Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) HRP-linked secondary antibodies were obtained from GE Healthcare (Piscataway, NJ, USA).
Rabbit anti pparγ
Manufactured by Thermo Fisher Scientific
Sourced in United States
Rabbit anti-PPARγ is a primary antibody that specifically binds to the Peroxisome Proliferator-Activated Receptor gamma (PPARγ) protein. PPARγ is a nuclear receptor that regulates gene expression involved in adipocyte differentiation, glucose and lipid metabolism. This antibody can be used for applications such as Western blotting, immunohistochemistry, and immunoprecipitation to detect and study the PPARγ protein.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti zo 1, by Thermo Fisher Scientific (1 mentions)
Mouse anti pecam 1, by Santa Cruz Biotechnology (1 mentions)
Mouse anti nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Mouse anti ho1, by Santa Cruz Biotechnology (1 mentions)
Mouse anti occludin, by Thermo Fisher Scientific (1 mentions)
Anti claudin 5, by Thermo Fisher Scientific (1 mentions)
Donkey anti rabbit na934, by GE Healthcare (1 mentions)
Mouse anti nqo 1, by Santa Cruz Biotechnology (1 mentions)
Rosiglitazone rsg a00183, by Adipogen (1 mentions)
Hrp conjugated anti mouse igg, by Bio-Rad (1 mentions)
Anti β actin, by Novus Biologicals (1 mentions)
Anti ucp1, by Abcam (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
2 protocols using rabbit anti pparγ
1
Rosiglitazone Modulates Endothelial Barrier
2
Western Blot Analysis of Adipogenic Proteins
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Total protein was extracted using RIPA buffer and separated on a 10% polyacrylamide gel, followed by transfer to a PVDF membrane. The primary antibodies used were rabbit anti-PPARγ (Invitrogen, Carlsbad, CA, USA), anti-C/EBPα (Novus Biologicals, Centennial, CO, USA), anti-UCP1 (Abcam, Cambridge, UK), and anti-β-actin (Novus Biologicals, Centennial, CO, USA). HRP-conjugated antimouse IgG or anti-rabbit IgG (Bio-Rad) were used as the secondary antibodies. The blots were treated with ECL substrate solutions and exposed to a ChemiDoc XRS System (Bio-Rad) to detect chemiluminescence.
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