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Pi rnase staining buffer

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The PI/RNase staining buffer is a laboratory reagent used in cell biology and molecular biology applications. It serves the core function of staining DNA with propidium iodide (PI) while simultaneously degrading RNA, allowing for the quantification of DNA content in cell samples.

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Lab products found in correlation

Cellquest software, by BD (2 mentions) Facscalibur flow cytometer, by BD (1 mentions) Facscan cell analyzer, by BD (1 mentions)

Close spelling variants of this product

PI staining buffer PI/RNase RNases

2 protocols using pi rnase staining buffer

1

Jagged1-mediated Cell Cycle Analysis

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Flow cytometry analysis was employed. Cells (50,000 cells/well in 6-wells-plates) were seeded on Jagged1 coated tissue culture surfaces and maintained in growth medium for 3 days. The cells were then harvested and fixed in cold 70% ethanol and stained with PI/RNase staining buffer (Sigma) for 30 min. The stained cells were analyzed by a FACSCalibur flow cytometer using CellQuest software (BD Bioscience).
Manokawinchoke J., Nattasit P., Thongngam T., Pavasant P., Tompkins K.A., Egusa H, & Osathanon T. (2017). Indirect immobilized Jagged1 suppresses cell cycle progression and induces odonto/osteogenic differentiation in human dental pulp cells. Scientific Reports, 7, 10124.
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2

Cell Cycle Analysis by Flow Cytometry

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After the cancer cells were treated with various drug combinations, they were fixed and re-suspended in a propidium iodide (PI)/RNase staining buffer (Sigma). The stained cells were then analyzed for DNA content using a FACScan cell analyzer equipped with Cellquest software (BD Biosciences).
Chen J., Guo J., Chen Z., Wang J., Liu M, & Pang X. (2016). Linifanib (ABT-869) Potentiates the Efficacy of Chemotherapeutic Agents through the Suppression of Receptor Tyrosine Kinase-Mediated AKT/mTOR Signaling Pathways in Gastric Cancer. Scientific Reports, 6, 29382.
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