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Annexin 5 fluorescein isothiocyanate and 7aad

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Annexin V–fluorescein isothiocyanate/and 7AAD is a laboratory reagent used in flow cytometry analysis. Annexin V binds to phosphatidylserine, a cell membrane component, while 7AAD is a DNA-binding dye. This combination is used to detect and quantify apoptotic and necrotic cells.

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Lab products found in correlation

Facscalibur cytometer, by BD (2 mentions) Cell quest computer software, by BD (2 mentions)

Close spelling variants of this product

7AAD and annexin-V Annexin V-fluorescein isothiocyanate Annexin V-fluorescein Annexin V

2 protocols using annexin 5 fluorescein isothiocyanate and 7aad

1

Apoptosis Analysis by Flow Cytometry

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Apoptotic rates were assessed with flow cytometry using the Annexin V–fluorescein isothiocyanate/and 7AAD (BD Pharmingen, San Diego, CA, USA). Samples were prepared according to manufacturer’s instructions. Flow cytometry analysis was performed using a FACS-Calibur cytometer using Cell Quest computer software (Becton Dickinson, Heidelberg, Germany).
Srdic-Rajic T., Santibañez J.F., Kanjer K., Tisma-Miletic N., Cavic M., Galun D., Jevric M., Kardum N., Konic-Ristic A, & Zoranovic T. (2017). Iscador Qu inhibits doxorubicin-induced senescence of MCF7 cells. Scientific Reports, 7, 3763.
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2

Apoptosis Quantification in Breast Cancer

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Examined breast cancer cells (MDA-MB-231) were stained with annexin V–fluorescein isothiocyanate/and 7AAD (BD Pharmingen, San Diego, CA, USA), and the samples were prepared following the specifications given by manufacturer. The population of apoptotic cells was analyzed by flow cytometry using a FACS Calibur cytometer and Cell Quest computer software (Becton Dickinson, Heidelberg, Germany).
Ruzic D., Ellinger B., Djokovic N., Santibanez J.F., Gul S., Beljkas M., Djuric A., Ganesan A., Pavic A., Srdic-Rajic T., Petkovic M, & Nikolic K. (2022). Discovery of 1-Benzhydryl-Piperazine-Based HDAC Inhibitors with Anti-Breast Cancer Activity: Synthesis, Molecular Modeling, In Vitro and In Vivo Biological Evaluation. Pharmaceutics, 14(12), 2600.
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