Ms grade acetonitrile

Manufactured by Avantor

Sourced in United States

MS grade acetonitrile is a high-purity solvent commonly used in various analytical applications, particularly in mass spectrometry (MS) techniques. It is a colorless, volatile liquid with a low viscosity and a characteristic odor. This product meets the stringent specifications required for use in sensitive analytical instruments and procedures.

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Lab products found in correlation

Dithiothreitol (dtt), by Merck Group (2 mentions) Ammonium bicarbonate, by Merck Group (2 mentions) Iodoacetamide, by Merck Group (2 mentions) Formic acid, by Merck Group (2 mentions) Triethylammonium bicarbonate, by Merck Group (1 mentions) Sequencing grade trypsin, by Promega (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Sequencing grade endoproteinase lys c, by Merck Group (1 mentions) Glycerol free peptide n glycosidase f, by New England Biolabs (1 mentions) 96 well plate, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Glutamine, by Thermo Fisher Scientific (1 mentions) Agarose, by Merck Group (1 mentions) Urea, by Merck Group (1 mentions) Mccoy s 5a media, by Thermo Fisher Scientific (1 mentions) Htc116 cells, by American Type Culture Collection (1 mentions) Nano pure system, by Thermo Fisher Scientific (1 mentions) C18 spin tips, by Thermo Fisher Scientific (1 mentions) Bovine pancreas tpck treated trypsin, by Merck Group (1 mentions)

Close spelling variants of this product

Acetonitrile (857 citations) LC-MS grade acetonitrile (79 citations) LC-MS grade acetonitrile (ACN) (16 citations) Acetonitrile (HPLC-MS grade) (11 citations) J.T. Baker Ultra LC/MS-grade acetonitrile (2 citations) LC-MS grade acetonitrile and water (2 citations) Acetonitrile HiPerSolv CHROMANORM LC-MS grade (2 citations)

2 protocols using ms grade acetonitrile

Protein Sample Preparation for Mass Spectrometry

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Liquid chromatography–MS grade acetonitrile (ACN), methanol, and water were obtained from Burdick & Jackson (Muskegon, MI). Reagents for protein chemistry, including SDS, ammonium bicarbonate, triethylammonium bicarbonate (TEAB), iodoacetamide, DTT, sequencing-grade endoproteinase Lys-C, and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO). Sequencing-grade trypsin was purchased from Promega (Madison, WI). Glycerol-free Peptide:N-glycosidase F was purchased from New England BioLabs (Ipswich, MA).

Moutin E.B., Bons J., Giavara G., Lourenco F., Pan D., Burton J.B., Shah S., Colombé M., Gascard P., Tlsty T., Schilling B, & Winton D.J. (2024). Extracellular Matrix Orchestration of Tissue Remodeling in the Chronically Inflamed Mouse Colon. Cellular and Molecular Gastroenterology and Hepatology, 17(4), 639-656.

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Proteomic Analysis of HCT116 Cells

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Bovine pancreas TPCK-treated trypsin, agarose, formic acid, urea, ammonium bicarbonate (NH4HCO3), dithiothreitol (DTT), and iodoacetamide (IAA) were purchased from Sigma-Aldrich (St. Louis, MO, USA); MS Grade Acetonitrile and MS Grade water were purchased from Honeywell – Burdick & Jackson (Muskegon, MI, USA). C18 SpinTips (product #89873) and 96-well plate were obtained from Thermo Scientific, and water was deionized by a Nano Pure system from Thermo Scientific (Marietta, OH, USA). McCoy’s 5A media, glutamine, and FBS were purchased from Life Technologies – Gibco (Grand Island, NY, USA); HTC116 cells were purchased from the ATCC (Manassas, VA, USA). Mammalian Cell PE Lysis Buffer for spheroid lysis was obtained from G-Biosciences (St. Louis, MO, USA). Complete protease inhibitor mini tablets were purchased from Roche (Indianapolis, IN, USA).

Feist P., Sun L., Liu X., Dovichi N.J, & Hummon A.B. (2015). Bottom-up proteomic analysis of single HCT 116 colon carcinoma multicellular spheroids. Rapid communications in mass spectrometry : RCM, 29(7), 654-658.

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