Probe Panc02 cells (1 × 107 (link)) were obtained, lysed, and sonicated as indicated. To generate the probe-coated beads, C-1 magnetic beads (Life Technologies) were co-incubated with the CircCCT3 probe for 2.5 hours at 25°C. Then, the CircCCT3 probe or oligo probe was co-incubated with cell lysates at 4° overnight. RNA was eluted and extracted by wash buffer and used for qRT-PCR. The CircCCT3 probe with biotinylation was synthesized and purchased from RiboBio (Guangzhou, PR China).The miR-613 was also tested using the same procedure.
Circcct3 probe
Manufactured by RiboBio
Sourced in China
The CircCCT3 probe is a laboratory equipment used for the detection and analysis of circular RNAs (circRNAs). It provides a tool for researchers to identify and study the expression patterns of specific circRNA sequences.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using circcct3 probe
1
Probing CircCCT3 and miR-613 in Panc02 Cells
2
CircCCT3 and miR-613 Interaction Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A RIP kit (Millipore, Billerica, MA, USA)was purchased for performing the RIP assay. PC cells were collected, and lysed by RIP lysis buffer. Then, the cell lysates were incubated with anti-Argonaute2 (Ago2)
or immunoglobulin G (IgG; negative control) antibody in RIP buffer at 4°C overnight. Co-precipitated RNA was isolate dafter digestion with 150 μL protease K. The expression of CircCCT3 and miR-613 was detected.
Pulldown assay with biotinylated CircCCT3 and miR-613 Probe Panc02 cells (1×10 7 ) were obtained, lysed, and sonicated as indicated. To generate the probecoated beads, C-1 magnetic beads (Life Technologies) were coincubated with the CircCCT3 probe for 2.5 h at 25℃. Then, the CircCCT3 probe or oligo probe was coincubated with cell lysates at 4℃ overnight. RNA was eluted and extracted by wash buffer and used for qRT-PCR. The CircCCT3 probe with biotinylation was synthesized and purchased from RiboBio (Guangzhou, PR China).The miR-613 was also tested using the same procedure.
or immunoglobulin G (IgG; negative control) antibody in RIP buffer at 4°C overnight. Co-precipitated RNA was isolate dafter digestion with 150 μL protease K. The expression of CircCCT3 and miR-613 was detected.
Pulldown assay with biotinylated CircCCT3 and miR-613 Probe Panc02 cells (1×10 7 ) were obtained, lysed, and sonicated as indicated. To generate the probecoated beads, C-1 magnetic beads (Life Technologies) were coincubated with the CircCCT3 probe for 2.5 h at 25℃. Then, the CircCCT3 probe or oligo probe was coincubated with cell lysates at 4℃ overnight. RNA was eluted and extracted by wash buffer and used for qRT-PCR. The CircCCT3 probe with biotinylation was synthesized and purchased from RiboBio (Guangzhou, PR China).The miR-613 was also tested using the same procedure.
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